Abstract: The present invention provides methods of selecting and transforming plant cells in large scale in vitro liquid cultures. In some methods of the invention, cells are selected that comprise a suppressive nucleic acid sequence that suppresses the effect of a target gene that impairs cellular function in the cell. In other embodiments, the methods are directed to identifying nucleic acids that encode polypeptides that physically interact with one another.
Type:
Application
Filed:
June 13, 2002
Publication date:
May 1, 2003
Applicant:
DNA Plant Technology Corporation
Inventors:
Dean Engler, Steven Scofield, Neal Gutterson, Peter John Balint-Kurti
Abstract: The present invention provides methods for eliminating plants containing non-T-DNA sequences derived from a T-DNA vector. More specifically, the present invention provides a method for killing plant cells that receive non-T-DNA sequences based on incorporation of a lethal polynucleotide sequence into the non-T-DNA portion of the vector.
Abstract: The present invention is directed to new pepper varieties that bear fruit that are sweet, red, and low-seeded and resemble a Jalapeño pepper in size and shape. Methods of making the plants are also provided.
Abstract: This invention provides methods for identifying plant cells that exhibit post-transcriptional gene silencing (PTGS) of a chosen gene. The methods involve the use of suppression-sensitive reporter genes that, when introduced into plant cells, are expressed at a lower level in cells that exhibit PTGS than in cells that are not silenced for the particular gene.
Type:
Grant
Filed:
September 17, 1998
Date of Patent:
September 17, 2002
Assignee:
DNA Plant Technology Corporation
Inventors:
John R. Bedbrook, Neal Gutterson, Paul W. Oeller
Abstract: The present invention is directed to methods for inhibiting the growth or killing cell in an organism, particularly plants. Genetically engineered cells and which allow for killing or provision of a beneficial effect to specified cells are also provided.
Abstract: The present invention provides methods for inhibiting target gene expression, by expressing in a cell a nucleic acid construct comprising a sense or antisense region having substantial sequence identity to a target gene, and a poly-dT sequence in a position 3′ to the sense or antisense region and 5′ of a polyadenylation signal.
Abstract: This invention relates to compositions and methods useful in the production of transgenic plants. In particular, the invention relates to nucleic acids that hybridizes under stringent conditions to a nucleic acid encoding a plant Gp2 protein that has the amino acid sequence depicted in SEQ ID NO:1 or SEQ ID NO:2. The invention also relates to the above-described nucleic acid operably linked to a heterologous or Gp2 plant promoter. In addition, the invention relates to transgenic plants containing the above-described nucleic acids.
Type:
Grant
Filed:
July 31, 1998
Date of Patent:
September 18, 2001
Assignee:
DNA Plant Technology Corporation
Inventors:
Catherine S. Baden, Pamela Dunsmuir, Kathleen Y. Lee
Abstract: Methods for Agrobacterium-mediated transformation and regeneration of strawberry plants are disclosed. Substitution of glucose or fructose for sucrose in various media preparations results in improved transformation efficiencies.
Type:
Grant
Filed:
January 15, 1999
Date of Patent:
August 14, 2001
Assignee:
(VPP Corporation) DNA Plant Technology Corporation
Inventors:
Seema Dhir, Maud A. W. Hinchee, Jeanne G Layton, Janette V. Oakes
Abstract: The present invention is directed to methods for producing plants containing alternate expression cassettes at a single locus in the plant genome. The two expression cassettes encode polypeptides which, when present in the same cell, are lethal to the cell. In preferred embodiments, the plant cell is an anther cell and the plant is male sterile.
Abstract: The present invention provides determinate, delayed-ripening cherry tomato plants. The plants are hybrids derived from a determinate, non-ripening parental line.
Abstract: The present invention provides determinate, delayed-ripening cherry tomato plants. The plants are hybrids derived from a determinate, non-ripening parental line.
Abstract: The present invention provides a method of producing a transformed banana plant (genus,Musa), in particular by tranforming embroygenic material, or the somatic embryos derived from a banana plant, through incubation with Agrobacterium cells carrying exogenous DNA sequence(s), and obtaining regenerated plants.
Type:
Grant
Filed:
July 16, 1997
Date of Patent:
October 17, 2000
Assignees:
DNA Plant Technology Corporation, Zeneca, Ltd.
Inventors:
Dean Engler, Neal Gutterson, Garry S. Nisbet
Abstract: This invention relates to compositions and methods useful in the production of transgenic plants. In particular, the invention relates to Group 2 (Gp2) plant promoter sequences and to expression cassettes containing Gp2 plant promoter sequences. The invention also relates to vectors and transgenic plants containing Gp2 plant promoter sequences that are operably linked to heterologous DNA sequences. In addition, the invention relates to methods of producing transgenic plants by using vectors containing Gp2 promoter sequences.
Type:
Grant
Filed:
December 6, 1996
Date of Patent:
November 9, 1999
Assignee:
DNA Plant Technology Corporation
Inventors:
Catherine S. Baden, Pamela Dunsmuir, Kathleen Y. Lee
Abstract: The present invention provides tomato plants exhibiting a delayed ripening phenotype. The plants of the invention comprise a T-DNA insert comprising a first sequence of from about nucleotide 149 to about nucleotide 1237 of a tomato Acc synthase gene and two inverted repeats of the first sequence. Integration of the T-DNA insert into the plant genome inhibits ethylene biosynthesis in the fruit.
Type:
Grant
Filed:
June 27, 1996
Date of Patent:
September 14, 1999
Assignee:
DNA Plant Technology Corporation
Inventors:
John R. Bedbrook, Pamela Dunsmuir, William J. Howie, Lawrence K. Joe, Kathleen Y. Lee
Abstract: The present invention is directed to methods for the genetic transformation of pineapple plant tissue with Agrobacterium. The present invention also provides for the regeneration of intact pineapple plants from the transformed tissue.
Abstract: The invention provides polynucleotides of the Capsicum annuum hemicellulase gene, and compositions and uses thereof for controlling plant development and other characteristics.
Abstract: The present invention provides compositions and methods for regulating vacuolar pH. Isolated DNA constructs comprising sequences substantially identical to a Ph gene are provided. The methods typically involve introducing the construct into a plant, whereby vacuolar pH is modified in the transgenic plant.
Type:
Grant
Filed:
February 16, 1996
Date of Patent:
June 8, 1999
Assignee:
DNA Plant Technology Corporation
Inventors:
George S. Chuck, Hugo K. Dooner, Neal Courtney-Gutterson, Janis Keller, Charanjit S. Nijjar, Edward J. Ralston
Abstract: Novel bacterial strains are described which produce and secrete chitinase as a result of the introduction of foreign DNA linked to a sequence encoding chitinase, an enzyme that degrades chitin. The bacterial strains can be used to provide protection for plants against chitinase sensitive plant pathogens. Methods of preparing the chitinase producing bacterial cells are also described. Methods of protecting plants from chitinase sensitive pathogens are also described.
Abstract: The present invention provides determinate, delayed-ripening cherry tomato plants. The plants are hybrids, such as line 94478 (ATCC Accession No. 97315), derived from the determinate, non-ripening parental line 91047-15 (ATCC Accession No. 97316).