Patents Assigned to DNA Script SAS
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Patent number: 11905541Abstract: The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides that include or enable a step of efficiently cleaving the polynucleotide products from its initiator using an endonuclease V activity and initiator with a 3?-penultimate deoxyinosine.Type: GrantFiled: June 10, 2022Date of Patent: February 20, 2024Assignee: DNA Script SASInventor: Sandrine Creton
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Patent number: 11661436Abstract: Methods for preparing 3?-O-amino-2?-deoxyribonucleoside-5?-triphosphates with reduced 3?-hydroxy-2?-deoxyribonucleoside-5?-triphosphate contamination by converting 3?-(N-acetone-oxime)-2?-deoxynucleoside triphosphate to 3?-O-amine-2?-deoxynucleoside triphosphate by treatment with an aryl-oxyamine and compositions produced therefrom.Type: GrantFiled: March 26, 2021Date of Patent: May 30, 2023Assignee: DNA SCRIPT SASInventors: Filip Wojciechowski, Thomas Ybert
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Publication number: 20230105977Abstract: The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides that include or enable a step of efficiently cleaving the polynucleotide products from its initiator using an endonuclease V activity and initiator with a 3?-penultimate deoxyinosine.Type: ApplicationFiled: June 10, 2022Publication date: April 6, 2023Applicant: DNA Script SASInventor: Sandrine Creton
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Publication number: 20220389048Abstract: The invention relates to a process for preparing a 3?-O-amino-ribonucleotide. It also relates to a compound of formula (III), and its use as a precursor for the synthesis of a 3?-O-amino-ribonucleotide. In formula (III), B is a nitrogenous base or a protected derivative thereof, G and G? are identical or different, and are a protecting group, and RN— is a phthalimido or an imino group.Type: ApplicationFiled: April 22, 2022Publication date: December 8, 2022Applicant: DNA Script SASInventors: Ivo SARAC, Cédric BARBOUX, Supaporn NIYOMCHON, Florent BEAUFILS, Martin BUESCHLEB
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Publication number: 20220186213Abstract: The invention is directed to methods for synthesizing oligonucleotides direction on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3?-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3? hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.Type: ApplicationFiled: March 4, 2022Publication date: June 16, 2022Applicant: DNA Script SASInventors: Xavier Godron, Adrian Horgan, Sylvain Gariel, Jeffrey Jeddeloh, Robert Nicol, Thomas Ybert
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Patent number: 11359221Abstract: The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides that include or enable a step of efficiently cleaving the polynucleotide products from its initiator using endonuclease V activity and initiator with a 3?-penultimate deoxyinosine.Type: GrantFiled: February 11, 2020Date of Patent: June 14, 2022Assignee: DNA Script SASInventor: Sandrine Creton
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Patent number: 11312742Abstract: The invention relates to a process for preparing a 3?-O-amino-ribonucleotide. It also relates to a compound of formula (III), and its use as a precursor for the synthesis of a 3?-O-amino-ribonucleotide. In formula (III), B is a nitrogenous base or a protected derivative thereof, G and G? are identical or different, and are a protecting group, and RN— is a phthalimido or an imino group.Type: GrantFiled: February 13, 2020Date of Patent: April 26, 2022Assignee: DNA Script SASInventors: Ivo Sarac, Cédric Barboux, Supaporn Niyomchon, Florent Beaufils, Martin Bueschleb
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Patent number: 11268091Abstract: The invention is directed to methods for synthesizing oligonucleotides directly on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3?-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3? hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.Type: GrantFiled: December 10, 2019Date of Patent: March 8, 2022Assignee: DNA Script SASInventors: Xavier Godron, Adrian Horgan, Sylvain Gariel, Jeffrey Jeddeloh, Robert Nicol, Thomas Ybert
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Patent number: 11208637Abstract: The present invention relates to variants of Terminal deoxynucleotidyl Transferase (TdT), each of which (i) has an amino acid sequence similarity to SEQ ID NO: 2. 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35 with corresponding amino acid substitutions, (ii) is capable of synthesizing a nucleic acid fragment without a template and (iii) is capable of incorporating a modified nucleotide into the nucleic acid fragment.Type: GrantFiled: July 10, 2020Date of Patent: December 28, 2021Assignees: DNA Script SAS, Institut Pasteur, National Center for Scientific Research (CNRS)Inventors: Elise Champion, Mikhael Soskine, Thomas Ybert, Marc Delarue
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Publication number: 20210300961Abstract: Methods for preparing 3?-O-amino-2?-deoxyribonucleoside-5?-triphosphates with reduced 3?-hydroxy-2?-deoxyribonucleoside-5?-triphosphate contamination by converting 3?-(N-acetone-oxime)-2?-deoxynucleoside triphosphate to 3?-O-amine-2?-deoxynucleoside triphosphate by treatment with an aryl-oxyamine and compositions produced therefrom.Type: ApplicationFiled: March 26, 2021Publication date: September 30, 2021Applicant: DNA Script SASInventors: Filip WOJCIECHOWSKI, Thomas YBERT
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Publication number: 20210254114Abstract: The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides that include or enable a step of efficiently cleaving the polynucleotide products from its initiator using endonuclease V activity and initiator with a 3?-penultimate deoxyinosine.Type: ApplicationFiled: February 11, 2020Publication date: August 19, 2021Applicant: DNA Script SASInventor: Sandrine Creton
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Publication number: 20210198661Abstract: The invention is directed to methods for synthesizing oligonucleotides direction on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3?-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3? hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.Type: ApplicationFiled: March 4, 2021Publication date: July 1, 2021Applicant: DNA Script SASInventors: Xavier Godron, Adrian Horgan, Sylvain Gariel, Jeffrey Jeddaloh, Robert Nicol, Thomas Ybert
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Publication number: 20210009994Abstract: The invention is directed to methods for synthesizing oligonucleotides direction on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3?-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3? hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.Type: ApplicationFiled: December 10, 2019Publication date: January 14, 2021Applicant: DNA Script SASInventors: Xavier Godron, Adrian Horgan, Sylvain Gariel, Jeffrey Jeddaloh, Robert Nicol, Thomas Ybert
