Patents Assigned to DNA Twopointo
  • Publication number: 20240052367
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel elements that further improve expression. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Application
    Filed: June 8, 2023
    Publication date: February 15, 2024
    Applicant: DNA TWOPOINTO INC.
    Inventors: Jeremy MINSHULL, Maggie LEE
  • Patent number: 11845936
    Abstract: The present invention provides methods and compositions for stable genetic modification of cultured mammalian cells. The genetic modifications can be used to produce cultured mammalian cells for therapeutic or diagnostic purposes.
    Type: Grant
    Filed: November 4, 2021
    Date of Patent: December 19, 2023
    Assignee: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Maggie Lee
  • Publication number: 20230323396
    Abstract: The invention provides inducible promoter systems and their components incorporating components of a tetracycline operon. By coordinating expression of different transcriptional units in these systems as a result of selection of promoters and/or linking the units into the same DNA molecule, these systems can achieve higher levels of expression of coding segments of interest, increased differential levels of expression between on- and off-states, and/or greater responsiveness to inducing agents than conventional systems.
    Type: Application
    Filed: December 16, 2022
    Publication date: October 12, 2023
    Applicant: DNA TWOPOINTO INC.
    Inventor: Jeremy Minshull
  • Publication number: 20230313187
    Abstract: The present invention provides methods and compositions for stable genetic modification of cultured mammalian cells. The genetic modifications can be used to produce cultured mammalian cells for therapeutic or diagnostic purposes.
    Type: Application
    Filed: September 3, 2021
    Publication date: October 5, 2023
    Applicant: DNA TWOPOINTO INC.
    Inventors: Jeremy MINSHULL, Maggie LEE, Varsha SITARAMAN, Oren BESKE, Ferenc BOLDOG
  • Publication number: 20230302054
    Abstract: Modified immune cells are provided, the modified immune cells expressing a heterologous polynucleotide comprising a nucleotide sequence encoding a function (e.g., at least one of persistence, proliferation, or cytotoxicity) booster, e.g., an apoptosis inhibitor. In one aspect, the modified T cells further comprise a chimeric antigen receptor. Methods, kits, and components for making and using the modified immune cells are also provided.
    Type: Application
    Filed: March 20, 2023
    Publication date: September 28, 2023
    Applicants: DNA Twopointo, The General Hospital Corporation
    Inventors: Yifang Shui, Jeremy Minshull, Maggie Lee, Feng Shi
  • Patent number: 11713468
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel elements that further improve expression. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Grant
    Filed: August 2, 2018
    Date of Patent: August 1, 2023
    Assignee: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Maggie Lee
  • Patent number: 11566262
    Abstract: The invention provides inducible promoter systems and their components incorporating components of a tetracycline operon. By coordinating expression of different transcriptional units in these systems as a result of selection of promoters and/or linking the units into the same DNA molecule, these systems can achieve higher levels of expression of coding segments of interest, increased differential levels of expression between on- and off-states, and/or greater responsiveness to inducing agents than conventional systems.
    Type: Grant
    Filed: March 21, 2022
    Date of Patent: January 31, 2023
    Assignee: DNA TWOPOINTO INC.
    Inventor: Jeremy Minshull
  • Patent number: 11401521
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Grant
    Filed: June 4, 2021
    Date of Patent: August 2, 2022
    Assignee: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
  • Patent number: 11396662
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Grant
    Filed: June 4, 2021
    Date of Patent: July 26, 2022
    Assignee: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
  • Publication number: 20220170044
    Abstract: The present invention provides methods and compositions for stable genetic modification of immune cells. The genetic modifications can be used to produce immune cells for therapeutic or diagnostic purposes.
    Type: Application
    Filed: February 7, 2020
    Publication date: June 2, 2022
    Applicants: DNA Twopointo, Inc., The General Hospital Corporation
    Inventors: Mark Cobbold, Maggie Lee, Jeremy Minshull, Feng Shi, Yifang Shui
  • Publication number: 20220112500
    Abstract: The present invention provides methods and compositions for stable genetic modification of cultured mammalian cells. The genetic modifications can be used to produce cultured mammalian cells for therapeutic or diagnostic purposes.
    Type: Application
    Filed: November 4, 2021
    Publication date: April 14, 2022
    Applicant: DNA TWOPOINTO INC.
    Inventors: JEREMY MINSHULL, FERENC BOLDOG, VARSHA SITARAMAN, MAGGIE LEE
  • Patent number: 11162102
    Abstract: The present invention provides methods and compositions for stable genetic modification of cultured mammalian cells. The genetic modifications can be used to produce cultured mammalian cells for therapeutic or diagnostic purposes.
    Type: Grant
    Filed: May 11, 2020
    Date of Patent: November 2, 2021
    Assignee: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Maggie Lee, Varsha Sitaraman, Ferenc Boldog
  • Patent number: 11060109
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Grant
    Filed: April 7, 2020
    Date of Patent: July 13, 2021
    Assignee: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
  • Patent number: 11060098
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Grant
    Filed: April 7, 2020
    Date of Patent: July 13, 2021
    Assignee: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
  • Patent number: 11060086
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Grant
    Filed: April 7, 2020
    Date of Patent: July 13, 2021
    Assignee: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
  • Publication number: 20210139919
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Application
    Filed: January 15, 2021
    Publication date: May 13, 2021
    Applicant: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Mark Welch, Sridhar Govindrajan, Maggie Lee, Kate Caves, Jon Ness
  • Patent number: 10927384
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Grant
    Filed: October 6, 2016
    Date of Patent: February 23, 2021
    Assignee: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Mark Welch, Sridhar Govindrajan, Maggie Lee, Kate Caves, Jon Ness
  • Publication number: 20200318135
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Application
    Filed: April 7, 2020
    Publication date: October 8, 2020
    Applicant: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
  • Publication number: 20200318121
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell.
    Type: Application
    Filed: April 7, 2020
    Publication date: October 8, 2020
    Applicant: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
  • Publication number: 20200318107
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Application
    Filed: April 7, 2020
    Publication date: October 8, 2020
    Applicant: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Sridhar Govindarajan, Maggie Lee