Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel elements that further improve expression. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Abstract: The present invention provides methods and compositions for stable genetic modification of cultured mammalian cells. The genetic modifications can be used to produce cultured mammalian cells for therapeutic or diagnostic purposes.
Abstract: The invention provides inducible promoter systems and their components incorporating components of a tetracycline operon. By coordinating expression of different transcriptional units in these systems as a result of selection of promoters and/or linking the units into the same DNA molecule, these systems can achieve higher levels of expression of coding segments of interest, increased differential levels of expression between on- and off-states, and/or greater responsiveness to inducing agents than conventional systems.
Abstract: The present invention provides methods and compositions for stable genetic modification of cultured mammalian cells. The genetic modifications can be used to produce cultured mammalian cells for therapeutic or diagnostic purposes.
Type:
Application
Filed:
September 3, 2021
Publication date:
October 5, 2023
Applicant:
DNA TWOPOINTO INC.
Inventors:
Jeremy MINSHULL, Maggie LEE, Varsha SITARAMAN, Oren BESKE, Ferenc BOLDOG
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel elements that further improve expression. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Abstract: The invention provides inducible promoter systems and their components incorporating components of a tetracycline operon. By coordinating expression of different transcriptional units in these systems as a result of selection of promoters and/or linking the units into the same DNA molecule, these systems can achieve higher levels of expression of coding segments of interest, increased differential levels of expression between on- and off-states, and/or greater responsiveness to inducing agents than conventional systems.
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Type:
Grant
Filed:
June 4, 2021
Date of Patent:
August 2, 2022
Assignee:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Type:
Grant
Filed:
June 4, 2021
Date of Patent:
July 26, 2022
Assignee:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
Abstract: The present invention provides methods and compositions for stable genetic modification of immune cells. The genetic modifications can be used to produce immune cells for therapeutic or diagnostic purposes.
Type:
Application
Filed:
February 7, 2020
Publication date:
June 2, 2022
Applicants:
DNA Twopointo, Inc., The General Hospital Corporation
Inventors:
Mark Cobbold, Maggie Lee, Jeremy Minshull, Feng Shi, Yifang Shui
Abstract: The present invention provides methods and compositions for stable genetic modification of cultured mammalian cells. The genetic modifications can be used to produce cultured mammalian cells for therapeutic or diagnostic purposes.
Type:
Application
Filed:
November 4, 2021
Publication date:
April 14, 2022
Applicant:
DNA TWOPOINTO INC.
Inventors:
JEREMY MINSHULL, FERENC BOLDOG, VARSHA SITARAMAN, MAGGIE LEE
Abstract: The present invention provides methods and compositions for stable genetic modification of cultured mammalian cells. The genetic modifications can be used to produce cultured mammalian cells for therapeutic or diagnostic purposes.
Type:
Grant
Filed:
May 11, 2020
Date of Patent:
November 2, 2021
Assignee:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Maggie Lee, Varsha Sitaraman, Ferenc Boldog
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Type:
Grant
Filed:
April 7, 2020
Date of Patent:
July 13, 2021
Assignee:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Type:
Grant
Filed:
April 7, 2020
Date of Patent:
July 13, 2021
Assignee:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Type:
Grant
Filed:
April 7, 2020
Date of Patent:
July 13, 2021
Assignee:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Type:
Application
Filed:
January 15, 2021
Publication date:
May 13, 2021
Applicant:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Mark Welch, Sridhar Govindrajan, Maggie Lee, Kate Caves, Jon Ness
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Type:
Grant
Filed:
October 6, 2016
Date of Patent:
February 23, 2021
Assignee:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Mark Welch, Sridhar Govindrajan, Maggie Lee, Kate Caves, Jon Ness
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Type:
Application
Filed:
April 7, 2020
Publication date:
October 8, 2020
Applicant:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell.
Type:
Application
Filed:
April 7, 2020
Publication date:
October 8, 2020
Applicant:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Type:
Application
Filed:
April 7, 2020
Publication date:
October 8, 2020
Applicant:
DNA TWOPOINTO INC.
Inventors:
Jeremy Minshull, Sridhar Govindarajan, Maggie Lee
Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel elements that further improve expression. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.