Abstract: A method for measuring concentration of dehydrogenase and substrate thereof in the presence of living cells, wherein an electron mediator that is hard to be reduced by living cells, for example, a compound represented by Formula (I), an electron mediator represented by the Formula (I) and a measurement reagent for dehydrogenase and substrate thereof including the electron mediator are disclosed. A-L-M??(I), wherein A represents a structural part containing at least one anionic group, M represents a structural part for mediating electron transfer and L represents a linker part for linking the A to the M.

Abstract: The present invention provides a carrier capable of highly efficiently introducing a compound into cells with low cytotoxicity, which contains peptide lipids represented by the following formula, and a method for introducing a compound into cells using the carrier: wherein R1 is an amino acid or peptide having 1-10 amino acid residues, R2 is a side chain of any amino acid, provided that R2 has a carboxyl group, the carboxyl group may be an ester with a hydrocarbon group having 1-30 carbon atoms, R3 is a hydrocarbon group having 1-30 carbon atoms.

Abstract: The purpose of the present invention is to provide a water-soluble tetrazolium compound that will form a water-soluble formazan exhibiting long-wavelength absorption and is stable in aqueous solution for a long period and suitable for the quantitative analysis of dehydrogenases or substrates thereof. Disclosed is a water soluble tetrazolium compound expressed by the following general formula (1): wherein each of R1 to R19 independently represents hydrogen atom; nitro group; sulfonate group or alkyl, alkoxy, sulfoalkyl or sulfoalkyloxy groups having 1 to 4 carbon atoms; provided that each of at least two of R1 to R19 independently represents sulfonate group; or sulfoalkyl or sulfoalkyloxy groups having 1 to 4 carbon atoms; and M represents an alkali metal or ammonium ion.

Abstract: Tetrazolium salt compounds represented by the following general formula (1): ##STR1## wherein R.sup.1 and R.sup.2 independently represent hydrogen atom, nitro group, cyano group, carboxyl group or a halogen atom, preferably nitro group; R.sup.3 represents an alkyl group or an alkoxyl group, preferably methyl group or methoxy group; and M represents an alkali metal or an ammonium. The compounds are useful for quantitative measurement of a dehydrogenase, and characterized to have high water-solubility and excellent storage stability in the state of an aqueous solution.

Abstract: An object of the present invention is to inhibit the influence of the reducing substances on the oxidative coloring reaction in biological components.A constitution of the present invention is that a compound having the free radical of the general formula (1) ##STR1## is previously added to the biological sample and then an incubation is carried out.An effect of the present invention is that the influence of the reducing substances on the oxidation reaction can be inhibited and, even when no reducing substance is present, there is no effect on the oxidation reaction.

Abstract: This invention offers the compounds which exhibit a coronary blood vessel dilating action which is long-acting.(Constitution)Novel imidazolinoxyl derivatives represented by a general formula (1). A compound A in which R is COOH is given in an example. ##STR1## (Merit) The compounds of the present invention can be directly administered, after diluting to a suitable concentration with a phosphate buffer or the like, to blood vessel which is required to be relaxed and dilated whereby a significant and long-acting relaxing action is resulted though, in a blood flow, they are quickly inactivated and excreted and no side effect is noted.

Abstract: Novel water-soluble methylenebis(dialkylaniline) derivatives of the following general formula and salts thereof are disclosed. They are useful as dye-forming substances in quantitative determination of peroxides in the copresence of peroxidase or the like. ##STR1## In the formula, R.sub.1 is hydrogen or a substituent selected from lower alkyl and C.sub.2-3 sulfoalkyl or hydroxysulfoalkyl; R.sub.2 is C.sub.2-3 sulfoalkyl or hydroxysulfoalkyl; and R.sub.3 is lower alkyl.

Abstract: A fluorescent compound, which is readily capable of forming a stable complex with a rare-earth metal ion having fluorescence with satisfactory intensity even in an aqueous system and long fluorescence lifetime when formed a complex with the rare-earth metal ion, a complex of the fluorescent compound and the rare-earth metal ion, and a labelled reagent made therefrom, together with a specific binding assay using said labelling agent.A fluorescent compound expressed by the following formula A, typically exemplified by 2,15-diaza[3,3](2,9)-1,10-phenanthrolinophane-N.sup.2,N.sup.15 -diacetic acid.

Abstract: N-sulfoalkylaniline derivatives in which at least one sulfoalkyl of 2 to 4 carbon atoms is attached to the nitrogen atom which are useful as dyestuff forming substances, a composition for determining the presence of peroxides using such compounds and the process for determination of peroxides using such compounds.

Abstract: A method for detecting and determining a volatile or volatilizable substance in an aqueous solution or liquid and an apparatus employed for this method are disclosed. The volatile component, vaporized from the solution through a water repellent and gas permeable membrane, is brought into contact with a dried or partially dried transparent film-like color developing carrier which is provided adjacent to the membrane, and the determination is carried out by the degree of color-change of the carrier. The apparatus comprises a cover plate with sample holes, a membrane, a color developing carrier, and a cover plate with observation windows. The members are fitted tightly together to form a single composite layer.