Abstract: A process for recovery of phytin with a higher purity comprises the steps of preparing LSW or CSL, inoculating a yeast into LSW or CSL, culturing the yeast therein to precipitate phytin and separating phytin by sedimentation. The method may comprise a further step of adding salt into CSL to accelerate the formation of phytin precipitate and increase the yield of phytin.
Abstract: An Escherichia coli (ATCC 68319) containing a gene encoding a novel maltogenic amylase of Bacillus licheniformis (BLMA), wherein said gene has a length of about 3.5 kb; said gene expresses a gene product capable of hydrolyzing cyclodextrin, pullulan, as well as starch at an optimum temperature of about 50.degree. C. at a pH of about 7; said gene product has sugar transferase activity in the presence of glucose; and said Escherichia coli produces said gene product optimally when grown in Luria broth, for 24 hours, at 37.degree. C.