Abstract: A sampler including a hood having a valve mounted at one end and a reciprocally related sleeve slidably mounted in the hood. a sample bottle having a septum is held in a bottle retainer that is slidably mounted on the hood. A pair of needles extend from the hood and communicate with a pair of bores that extend through the hood. One bore is vented to the exterior while the other bore communicates with channels in the valve. The needles may be sterilized, in a simple manner, prior to and/or after sampling. In a special embodiment of the sampler it is possible to sterilize the needles after sampling and before the needles contact the ambient air.