Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.
Type:
Grant
Filed:
November 11, 2014
Date of Patent:
July 9, 2019
Assignees:
Dow AgroSciences LLC, Sangamo Therapeutics, Inc.
Inventors:
Manju Gupta, Asha M. Palta, Stephen Novak, Fyodor Urnov, Sunita Gopalan
Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran and hemipteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran and hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran and hemipteran pests, and the plant cells and plants obtained thereby.
Type:
Grant
Filed:
October 4, 2016
Date of Patent:
July 9, 2019
Assignee:
Dow AgroSciences LLC
Inventors:
Kenneth E. Narva, Elane Fishilevich, Murugesan Rangasamy, Meghan L. Frey, Wendy Lo, Sarah E. Worden, Premchand Gandra
Abstract: The subject invention relates in part to Cry34Ab/35Ab in combination with Cry3Aa. The subject invention relates in part to the surprising discovery that combinations of Cry34Ab/Cry35Ab and Cry3Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (Diabrotica spp.) population. As one skilled in the art will recognize with the benefit of this disclosure, corn plants producing these insecticidal Cry proteins will be useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. Plants (and acreage planted with such plants) that produce these two insecticidal protein systems are included within the scope of the subject invention.
Type:
Application
Filed:
March 15, 2019
Publication date:
July 4, 2019
Applicant:
DOW AGROSCIENCES LLC
Inventors:
Kenneth E. Narva, Thomas Meade, Kristin J. Fencil, Huarong Li, Timothy D. Hey, Aaron T. Woosley, Monica Britt Olson
Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran and/or hemipteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran and/or hemipteran pests, and the plant cells and plants obtained thereby.
Type:
Grant
Filed:
December 13, 2016
Date of Patent:
June 25, 2019
Assignee:
Dow AgroSciences LLC
Inventors:
Kenneth E. Narva, Elane Fishilevich, Murugesan Rangasamy, Meghan L. Frey, Wendy Lo, Sarah E. Worden, Premchand Gandra
Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.
Type:
Grant
Filed:
January 6, 2016
Date of Patent:
June 18, 2019
Assignee:
Dow AgroSciences LLC
Inventors:
Yunxing Cory Cui, Jill Bryan, Donald Maum, Greg Gilles, Terry Wright, Jennifer Hamilton, Nicole Arnold, Nathan VanOpdorp, Tina Kaiser, Ning Zhou
Abstract: The present disclosure describes a method of coupling a first compound to a second compound, the method comprising: providing the first compound having a fluorosulfonate substituent; providing the second compound comprising an amine; and reacting the first compound and the second compound in a reaction mixture, the reaction mixture including a catalyst having at least one group 10 atom, the reaction mixture under conditions effective to couple the first compound to the second compound. The present disclosure further describes a one-pot method for coupling a first compound to a second compound.
Type:
Application
Filed:
February 8, 2019
Publication date:
June 13, 2019
Applicant:
Dow AgroSciences LLC
Inventors:
Patrick S. Hanley, Thomas P. Clark, Matthias S. Ober, William J. Kruper
Abstract: The present application provides processes for making pesticidal compounds and compounds useful both as pesticides and in the making of pesticidal compounds.
Type:
Grant
Filed:
May 8, 2018
Date of Patent:
June 11, 2019
Assignee:
Dow AgroSciences LLC
Inventors:
Qiang Yang, Beth Lorsbach, Gregory Whiteker, Gary Roth, Carl DeAmicis, Thomas Clark, Kaitlyn Gray, Belgin Canturk, Elisabeth J. Kane, Yu Zhang, Joseck M. Muhuhi
Abstract: This disclosure relates to the field of molecules having pesticidal utility against pests in Phyla Arthropoda, Mollusca, and Nematoda, processes to produce such molecules, intermediates used in such processes, pesticidal compositions containing such molecules, and processes of using such pesticidal compositions against such pests. These pesticidal compositions may be used, for example, as acaricides, insecticides, miticides, molluscicides, and nematicides. This document discloses molecules having the following formula (“Formula One”).
Type:
Application
Filed:
January 18, 2019
Publication date:
June 6, 2019
Applicant:
Dow AgroSciences LLC
Inventors:
Ronald J. Heemstra, Ronald Ross, JR., Timothy P. Martin, Nicolaas Vermeulen, John F. Daeuble, SR., Joseph D. Eckelbarger, Alex Nolan, Kaitlyn Gray, David A. Demeter, Ricky Hunter, Tony K. Trullinger
Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3?UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.
Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays KN1 gene. Some embodiments relate to a promoter from a Zea mays KN1 gene that functions in plants to promote transcription of operably linked nucleotide sequences.
Type:
Application
Filed:
February 11, 2019
Publication date:
May 30, 2019
Applicant:
Dow AgroSciences LLC
Inventors:
John Davies, David Mann, James Patrick Connell, William T. Beeson, IV
Abstract: Provided herein are 2-(2,4-difluorophenyl)-1,1-difluoro-1-(5-substituted-pyridin-2-yl)-3-(1H-tetrazol-1-yl)propan-2-ols and 1-(2,4-difluorophenyl)-2,2-difluoro-2-(5-substituted-pyridin-2-yl)ethanones and processes for their preparation.
Type:
Grant
Filed:
February 1, 2018
Date of Patent:
May 28, 2019
Assignee:
Dow Agrosciences, LLC
Inventors:
William J. Hoekstra, Nakyen Choy, Carl DeAmicis, Daniel Knueppel, Jim Renga, Michael T. Sullenberger, Gregory Whiteker, Yuanming Zhu, Gary D. Gustafson
Abstract: This disclosure relates to the field of molecules having pesticidal utility against pests in Phyla Arthropoda, Mollusca, and Nematoda, processes to produce such molecules, intermediates used in such processes, pesticidal compositions containing such molecules, and processes of using such pesticidal compositions against such pests. These pesticidal compositions may be used, for example, as acaricides, insecticides, miticides, molluscicides, and nematicides. This document discloses molecules having the following formula (“Formula One”).
Type:
Application
Filed:
January 18, 2019
Publication date:
May 23, 2019
Applicant:
Dow AgroSciences LLC
Inventors:
Ronald J. Heemstra, Ronald Ross, JR., Timothy P. Martin, Nicolaas Vermeulen, John F. Daeuble, SR., Joseph D. Eckelbarger, Alex Nolan, Kaitlyn Gray, David A. Demeter, Ricky Hunter, Tony K. Trullinger
Abstract: This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3?UTR from Arabidopsis thaliana Ubiquitin C9 gene. Some embodiments relate to a 3? UTR from a Arabidopsis thaliana Ubiquitin C9 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.
Type:
Grant
Filed:
September 15, 2016
Date of Patent:
May 21, 2019
Assignee:
Dow AgroSciences LLC
Inventors:
Manju Gupta, Sara Bennett, Andrew F. Worden
Abstract: Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.
Type:
Grant
Filed:
October 13, 2016
Date of Patent:
May 14, 2019
Assignees:
Dow AgroSciences LLC, Sangamo Therapeutics, Inc.
Inventors:
W. Michael Ainley, Steven R. Webb, Jayakumar P. Samuel, Dmitry Y. Guschin, Jeffrey C. Miller, Lei Zhang
Abstract: Compounds formed by combining a carboxylic acid herbicide with N—((C1-C16) alkyl or arylalkyl) tri((C1-C16) alkyl)ammonium hydroxide have herbicidal activity on an acid equivalent basis at least as active as the commercially used carboxylic acid herbicide salts, but are less volatile.
Type:
Grant
Filed:
September 1, 2017
Date of Patent:
May 7, 2019
Assignee:
Dow AgroSciences LLC
Inventors:
Vincent J. Kramer, David G. Ouse, Norman R. Pearson, Holger Tank, Mark W. Zettler
Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays KN1 gene. Some embodiments relate to a promoter from a Zea mays KN1 gene that functions in plants to promote transcription of operably linked nucleotide sequences.
Type:
Grant
Filed:
October 20, 2016
Date of Patent:
May 7, 2019
Assignee:
Dow AgroSciences LLC
Inventors:
John Davies, David Mann, James Patrick Connell, William T. Beeson, IV
Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of pathogens through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in pathogens. The disclosure also concerns methods for applying dsRNA through formulations and/or transgenic plants that express nucleic acid molecules useful for the control of pathogens, and the plant cells and plants obtained thereby.
Type:
Application
Filed:
October 3, 2018
Publication date:
May 2, 2019
Applicant:
Dow AgroSciences LLC
Inventors:
Javier A. Delgado, Justin M. Lira, Michael T. Sullenberger, Robert Cicchillo
Abstract: The invention relates to methods and systems taking advantage of bioinformatic investigations to identify candidate signature peptides for quantitative multiplex analysis of complex protein samples from plants, plant parts, and/or food products using mass spectroscopy. Provided are use and methods for selecting candidate signature peptides for quantitation using a bioinformatic approach. Also provided are systems comprising a chromatography and mass spectrometry for using selected signature peptides.
Type:
Application
Filed:
August 11, 2015
Publication date:
May 2, 2019
Applicant:
Dow AgroSciences LLC
Inventors:
Trent James Oman, Barry William Shafer, Ryan Christopher Hill, Guomin Shan
Abstract: As disclosed herein, optimal native genomic loci have been identified in monocot plants, such as maize plants, that represent best sites for targeted insertion of exogenous sequences.
Type:
Grant
Filed:
November 3, 2014
Date of Patent:
April 30, 2019
Assignee:
Dow AgroSciences LLC
Inventors:
Lakshmi Sastry-Dent, Zehui Cao, Shreedharan Sriram, Steven R. Webb, Debra L. Camper, Navin Elango