Abstract: A device for automated or manual group separations of complex biological mixtures and methods of use are described. The device is a gel filtration pipette tip having a size exclusion media therein and held in place by a filter at the distal end of the pipette tip and an optional removable or pierceable barrier at the proximal end of the pipette tip. The size exclusion media is wetted by aspirating a solvent from the bottom of the pipette tip and equilibrating the solvent with the size exclusion media. The solvent is then allowed to gravity drain from the pipette tip, allowing the size exclusion media to settle and reproducibly pack into a homogenous gel. Gel filtration separations can then be performed with the gelled size exclusion media.

Abstract: A device for filtration or extraction using at least one filtration membrane is disclosed. The device is shaped like a Buchner funnel, but small enough to fit with wide bore pipette tips, slip tip syringes or an adaptor of a robotic liquid handler or pipettor. It also houses one or more filtration membranes and or a separation resin. A reservoir adaptor can be added to the top in a fluid tight manner to provide a removable large volume container where needed for larger samples, and a gasket adaptor allows the reservoir to be connected to other devices in a fluid tight manner.

Abstract: A device for automated separations of complex biological mixtures and methods of use are described. The device is a size exclusion chromatography pipette tip (SEC tip) having a size exclusion media therein and held in place by a filter at the distal end of the pipette tip and an optional removable or pierceable barrier at the proximal end of the SEC tip. The size exclusion media is wetted by aspirating a solvent from the bottom of the pipette tip and equilibrating the solvent with the size exclusion media. Aspiration and dispensing of excess solvent are at 5-25 ?L/sec, thereby avoiding the creation of any bubbles in the resulting SEC column. Once excess solvent drains from the pipette tip, the size exclusion media settles and reproducibly pack into a homogenous gel. Separations can then be performed with the gelled SEC column.

Abstract: A pipette tip for extracting nucleic acid, said pipette tip containing a glass plug made of a porous silica material with porosities of 5 to 20 ?m and having a diameter of at least 0.8 mm and a thickness of at least 0.2 mm.

Abstract: Devices and methods for performing pre-analysis sample processing of biological and chemical samples using robotic liquid handlers are disclosed. Methods for solid phase extraction, protein precipitation and filtration of biological and chemical samples using automation and the devices in a rapid and convenient way are described.

Abstract: Devices and methods for performing pre-analysis sample processing of biological and chemical samples using robotic liquid handlers are disclosed. Methods for solid phase extraction, protein precipitation and filtration of biological and chemical samples using automation and the devices in a rapid and convenient way are described.