Abstract: A device for automated or manual separations of complex chemical or biological mixtures and methods of use are described. The device is a chromatography pipette tip or column having a size exclusion media therein and held in place by a hydrophilic filter at the distal end of the pipette tip and an optional removable or pierceable barrier at the proximal end of the pipette tip. The size exclusion media is wetted by simply placing the tip into solvent and allowing capillary action to flow through the filter and into the SEC resin. The solvent is then allowed to gravity drain from the pipette tip, allowing the size exclusion media to reproducibly pack into a homogenous gel. Separations can then be performed with the gelled size exclusion media.

Abstract: A process and system for automated extractions using a vacuum system is disclosed. The process includes the use of the robotic liquid handler's pipetting head for performing the elution step, thereby minimizing potential cross-contamination and improving throughput. The various disposable devices are designed to meet with each other at an angle of 7-90° from vertical to provide an air-tight, yet easily reversible seal. They are designed to fit together with minimal wobble, ensuring placement to allow a vacuum seal. An optional protective sheath is also provided to protect columns from tip contamination, the sheath not requiring the vacuum seal.

Abstract: Devices and methods for performing pre-analysis sample processing of biological and chemical samples using robotic liquid handlers are disclosed. Methods for solid phase extraction, protein precipitation and filtration of biological and chemical samples using automation and the devices in a rapid and convenient way are described.

Abstract: Devices and methods for performing pre-analysis sample processing of biological and chemical samples using robotic liquid handlers are disclosed. Methods for solid phase extraction, protein precipitation and filtration of biological and chemical samples using automation and the devices in a rapid and convenient way are described.