Abstract: An augmented reality with real-time interactive analysis method and a system thereof are provided. The method includes: starting an application from a portable electronic device by a user; reading a checklist by the application; identifying basic data fields and biochemical fields of the checklist; recording the basic data fields and the data in the biochemical fields and converting them into digital data; determining whether the data of the biochemical fields meets a preset standard value; according to whether the results conform to the preset standard value, the application produces correspondingly a first signal or a second signal; the application generating an augmented reality checklist image and adding a signal field to each biochemical field; and displaying the first signal or the second signal corresponding to the biochemical fields in the signal field; wherein each preset standard value is pre-stored in the application.