Abstract: This invention relates to a method of producing one or more human milk oligosaccharides (HMOs), in particular LNT and/or LNnT, in a genetically engineered cell comprising an enhanced oligosaccharide transport capability. The genetically modified cell comprises a series of genetic modification which enable the production of one or more HMO(s), and a series of genetic modification that enhances the transport of lactose and produced HMO(s).

Abstract: The present disclosure provides a method for increasing production of a polyunsaturated fatty acid, especially eicosapentaenoic acid (EPA), in a microorganism, comprising adjusting the macro-and/or micronutrients during fermentation. The invention includes any culture, biomass, and oil produced by the method.

Abstract: A computer-based method for determining a sunscreen composition comprising a plurality of UV filter substances, comprises the steps of selecting at least one constraint for at least one characteristic of the composition to be determined (step 30), the at least one constraint comprising a sunscreen performance target; selecting an optimization objective from a plurality of optimization objectives (step 50); and automatically determining the sunscreen composition as a composition of filter substances from a set of filter substances (step 100), the composition meeting the at least one constraint and being optimized with respect to the selected optimization objective. The automatic determination comprises the steps of generating a plurality of candidate compositions, determining a sunscreen performance of the candidate compositions using a performance simulation tool and comparing the determined sunscreen performance of the candidate compositions with the sunscreen performance target.

Abstract: The present invention is directed to isolated nucleic acid molecules and polypeptides of thraustochytrid polyunsaturated fatty acid (PUFA) synthases involved in the production of PUFAs, including PUFAs enriched in docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof. The present invention is directed to vectors and host cells comprising the nucleic acid molecules, polypeptides encoded by the nucleic acid molecules, compositions comprising the nucleic acid molecules or polypeptides, and methods of making and uses thereof.

Abstract: The present disclosure relates to the production of sialylated Human Milk Oligosaccharides (HMOs), in particular to the biosynthetic production of 3?-sialyllactose (3?SL), and to genetically engineered cells and methods suitable for said production.

Abstract: Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Abstract: The present invention relates to a method for reducing fructan in a fructan-containing food product, including adding an invertase belonging to enzyme classification EC 3.2.1.26 to the food product, and incubating the food product with the invertase, wherein fructan in the fructan-containing food product is hydrolysed. The invention further relates to the use of an invertase belonging to enzyme classification EC. 3.2.1.26 for the preparation of a medicament or a dietary supplement for the treatment of a person suffering from irritable bowel syndrome. Invertase (EC 3.2.1.26) may optionally by further combined with inulinase (EC3.2.1.7) and/or beta-fructosidase (EC 3.2.1.80).

Abstract: The present invention relates to animal feed compositions comprising at least one probiotic and polypeptides having muramidase activity.

Abstract: Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Abstract: The presence of either a calcium source or a carbonate source with GH30 xylanses significantly improves the release of xylan oligomers from maize and other feeds compared to the same xylanases without the calcium or carbonate source. Animal feed and animal feed additives comprising a combination of one or more polypeptides having xylanase activity; and one or more sources of calcium; and/or one or more sources of carbonate, wherein the polypeptide is a GH30 xylanase provide for improved xylan release from feedstuff.

Abstract: The disclosure relates to methods of making an osteoconductive polymer article for use as an orthopedic implant comprises steps of forming an article from a biocompatible, non-biodegradable polymer, the article comprising a non-flat surface with roughness Ra of at least 5 ?m; providing a dispersion of bioactive ceramic particles of particle size at most 10 ?m in a first solvent comprising a solvent for the polymer; coating at least the non-flat surface with the dispersion in at least one step; and rinsing the coated article with a second solvent being a non-solvent for the polymer to substantially remove the first solvent.

Abstract: The present invention is related to production of retinyl esters, such as in particular retinyl acetate, an important building block for production of vitamin A. The retinyl esters might be generated via enzymatic conversion of retinol which process includes the use of enzymes with acetyl-transferase (ATF) activity, thus acetylating retinol into retinol/retinyl acetate. Said process is particularly useful for biotechnological production of vitamin A.

Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified yeast strains expressing enzymes having improved C-8 sterol isomerase activity leading to increased ratios of 7-DHC in the sterol mix.

Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified host strains expressing enzymes having improved C-5 sterol 5 desaturase activity and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.

Abstract: An animal feed comprising an animal feed additive, said additive comprising co-granules, each co-granule comprising at least two classes of enzymes, said co-granules comprising a core unit and a protective coating such that the dose of each of the enzymes in the feed is set in each granule rather than by the level of homogeneity of single-enzyme granules or by the efficiency of the mixing.

Abstract: The invention provides recombinant host organisms (e.g., plants) genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins (e.g., PPTase and/or ACoAS) that allow for and/or improve the production of PUFAs in the host organism. The present invention also relates to methods of making and using such organisms (e.g., to obtain PUFAs) as well as products obtained from such organisms (e.g., oil and/or seed).

Abstract: The subject disclosure features, in one aspect, a method for producing lipids enriched for EPA, comprising modifying a microalga to increase expression of PFA1, and/or PFA3, and culturing the modified microalga under conditions which allow the expression of PFA1, and/or PFA3, wherein lipids enriched for EPA are produced. Also featured is a recombinant microalga in which PFA1, and/or PFA3, is overexpressed. Such recombinant microalgae have been demonstrated herein to produce very favorable fatty acid lipid profiles (e.g., increased levels of EPA, increased ratio of EPA:DHA, decreased levels of DPA n-6, etc.).

Abstract: The current invention relates to a method of separating lipids from a lipids containing biomass by demulsification under mild conditions, to an oil and to a delipidated biomass as obtained by such a method.

Abstract: The current invention relates to a method of separating polyunsaturated fatty acids containing lipids from a lipids containing biomass.

Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto.