Abstract: A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-?-d-xylanase) and a ?-glucanase (and optionally a further fibre degrading enzyme).
Type:
Grant
Filed:
July 6, 2021
Date of Patent:
March 19, 2024
Assignee:
DUPONT NUTRITION BIOSCIENCES APS
Inventors:
Mai Faurschou Isaksen, Marion Bernardeau, Luis Fernando Romero Millan, Elijah G. Kiarie, Susan Lund Arent, Päivi Nurminen, Sofia Forssten, Daniel Petri, Elizabeth Ann Galbraith, Mari Ellen Davis
Abstract: This invention relates to bacterial strains, compositions comprising bacterial strains as well as methods and uses of said strains and compositions for preventing or treating illness and/or symptoms associated with a coronavirus in a subject in need thereof.
Type:
Grant
Filed:
April 1, 2022
Date of Patent:
January 30, 2024
Assignee:
DUPONT NUTRITION BIOSCIENCES APS
Inventors:
Sebastien Guery, Markus Lehtinen, Sinikka Anneli Latvala, Liisa Lehtoranta, Bryan-James Zabel, Wesley William Morovic, Charles Budinoff, Scott D. Power
Abstract: An isolated and/or purified ?-glucanotransferase from Exiguobacterium acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the ?-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the ?-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.
Abstract: The present invention relates to a method of making a dough, said method comprises admixing a dough component, a phospholipase A2 enzyme which acts on N-acyl phosphatidyl ethanolamine at the sn2 position, and an enzyme that acts on a polar lipid at the sn1 position. A food enzyme composition comprising: a phospholipase A2 enzyme which is capable of acting on N-acylphosphatidyl ethanolamine at the sn2 position; and an enzyme that acts on a polar lipid at the sn1 position is also taught.
Type:
Grant
Filed:
April 28, 2021
Date of Patent:
December 19, 2023
Assignee:
DuPont Nutrition Biosciences APS
Inventors:
Jorn Borch Soe, Rene Mikkelsen, Tina Lillan Joergensen
Abstract: A phospholipase A1 characterized by having an sn1/sn2 specificity ratio of about 55/45 or greater wherein said phospholipase A1 has a lysophospholipase/phospholipase activity ratio of less than 0.02 is presented in conjunction with methods for use in lipid containing food matrix, baking and making dough with the phospholipase and also including baking improvers using the disclosed phospholipase A1.
Abstract: Methods and compositions are provided for the use of anti-CRISPR (ACR) proteins in plants, including modulation of Cas endonuclease activity, improvement of frequency of homologous recombination, control of Cas endonuclease activity during various cell cycles, spatial and/or temporal regulation of Cas endonuclease activity in plants, usage in gene activation or repression, as well as reduction of off-target polynucleotide cleavage.
Type:
Grant
Filed:
April 24, 2018
Date of Patent:
August 22, 2023
Assignees:
DUPONT NUTRITION BIOSCIENCES APS, UNIVERSITE LAVAL (CANADA)
Inventors:
Christophe Fremaux, Philippe Horvath, Alexander Hynes, Marie-Laurence Lemay, Sylvain Moineau, Dennis A. Romero, Geneviève Rousseau, Joshua K Young
Abstract: The invention is directed to a lactose-positive, galactose-negative, Streptococcus thermophilus strain releasing glucose during milk fermentation. This Streptococcus thermophilus strain carries mutation in the glcK gene, in the ccpA gene, in the lacZ gene and/or in the ptsH gene, and optionally in one or more genes, in particular one gene, encoding a protein of the mannose-glucose-specific PTS. The invention also concerns a composition comprising at least one, lactose-positive, galactose-negative, Streptococcus thermophilus strain of the invention, and the use of this strain or composition to manufacture a fermented dairy product.
Type:
Grant
Filed:
December 21, 2018
Date of Patent:
August 15, 2023
Assignee:
DUPONT NUTRITION BIOSCIENCES APS
Inventors:
Armelle Cochu-Blachere, Christophe Fremaux, Thomas Desfougeres
Abstract: The present disclosure provides methods, compositions, apparatuses and kits comprising two or more distant related glucoamylases in mashing having a synergistic effect on the released fermentable DPI sugar. Increased levels of DPI being primarily glucose is of high importance for high attenuation brewing applied for brewing low carb or light beers. In some embodiments, the present disclosure provides methods, apparatuses; compositions and kits for the use of two or more glucoamylases to increase the apparent specific activity in the mashing operation.
Abstract: Compositions and methods are provided comprising acetolactate decarboxylase (ALDC) enzyme variants having higher specific activity. Composition and method are provided where the ALDC variants are used in combination with metal ions to further increase stability and/or activity.
Abstract: Certain embodiments of the disclosure are directed to the expression/production of ?-galactosidases in recombinant Bacillus spp. host cells having reduced or eliminated para-nitrobenzylesterase activity.
Abstract: The present invention relates to a culture or kit-of-part comprising a Lactobacillus delbrueckii subsp lactis strain, and uses thereof to manufacture a cereal-based lactic acid-fermented product, in particular a maize-based lactic acid-fermented product such as mahewu.
Abstract: The present disclosure provides polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. In some embodiments, the disclosure also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. In some embodiments, the present disclosure further provides to methods of obtaining protein hydrolysates useful as flavor improving agents.
Type:
Grant
Filed:
June 27, 2016
Date of Patent:
October 18, 2022
Assignee:
Dupont Nutrition Biosciences APS
Inventors:
Karsten Matthias Kragh, Ernst Meinjohanns, Thomas Eisele, Peter Edvard Degn, Steffen Yde Bak
Abstract: A method for Inhibiting or Delaying the Growth of Enterococcus spp. in Animals using at least one Bacillus-based component is disclosed herein.
Abstract: A method for the production of a hydrolysate comprising: (a) admixing at least one protein or a portion thereof with: (A) at least one endoprotease; and (B) (a?) at least one proline tolerant tripeptidyl peptidase or fermentate comprising a proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at P1; and synthetic amino acids at P1; or (b?) at least one proline tolerant tripeptidyl peptidase having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at PV; and synthetic amino acids at PV; and (b) recovering the hydrolysate.
Type:
Application
Filed:
November 24, 2021
Publication date:
June 9, 2022
Applicant:
DuPont Nutrition Biosciences APS
Inventors:
Karsten Matthias KRAGH, Ernst MEINJOHANNS, Andrei MIASNIKOV, Maria MA, Thomas EISELE, Svend HAANING, Peter Edvard DEGN, Steffen Yde BAK
Abstract: The invention relates to a polynucleotide comprising a lacZ gene (lacZFS) encoding a ?-galactosidase characterized by a particular profile regarding its efficiency of hydrolysis of lactose. The invention is also directed to a Streptococcus thermophilus strain comprising a lacZFS allele and bacterial composition thereof, and their use to obtain fermented milk not undergoing post-acidification.
Type:
Application
Filed:
March 13, 2020
Publication date:
June 2, 2022
Applicant:
DUPONT NUTRITION BIOSCIENCES APS
Inventors:
Anais JEDRZEJOWSKI, Christophe FREMAUX, Sabine VAN DILLEN, Thomas DESFOUGERES, Max Charles JODEAU, Damien LUGAND
Abstract: The present invention relates to polypeptides, specifically polypeptides having transgalactosylating activity and nucleic acids encoding these, and their uses in e.g. dairy product.
Type:
Application
Filed:
September 7, 2021
Publication date:
May 26, 2022
Applicant:
DuPont Nutrition Biosciences ApS
Inventors:
MORTEN KROG LARSEN, Jacob FLYVHOLM CRAMER
Abstract: The present invention relates to a lactic acid bacterium, in particular a strain of the Streptococcus genus, with reduced sensitivity to cos-type bacteriophages. The invention also relates to methods to engineer these lactic acid bacteria as well as their use to ferment milk.
Type:
Application
Filed:
February 13, 2020
Publication date:
April 21, 2022
Applicant:
DUPONT NUTRITION BIOSCIENCES APS
Inventors:
Christophe FREMAUX, Philippe HORVATH, Armelle COCHU-BLACHÈRE, Dennis ROMERO, Sylvain MOINEAU, Simon LABRIE
Abstract: A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-?-d-xylanase) and a ?-glucanase (and optionally a further fibre degrading enzyme).
Type:
Application
Filed:
July 6, 2021
Publication date:
February 10, 2022
Applicant:
DUPONT NUTRITION BIOSCIENCES APS
Inventors:
Mai Faurschou Isaksen, Marion Bernardeau, Luis Fernando Romero Millan, Elijah G. Kiarie, Susan Arent Lund, Päivi Nurminen, Sofia Forssten, Daniel Petri, Elizabeth Ann Galbraith, Mari Ellen Davis
Abstract: The present invention relates to the solubilisation of cereal bran, for preparing compositions comprising soluble fractions of cereal bran and the use of these compositions comprising solubilised cereal bran for the preparation of food products, such as bread.