Abstract: A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.
Type:
Grant
Filed:
December 13, 2018
Date of Patent:
March 10, 2020
Assignee:
DUPONT US HOLDING, LLC
Inventors:
Ryan L Frisch, Xiaochun Fan, Seung-Pyo Hong, Ethel Noland Jackson
Abstract: Non-conventional yeasts are disclosed herein comprising at least one RNA-guided endonuclease (RGEN) comprising at least one RNA component that does not have a 5?-cap. This uncapped RNA component comprises a sequence complementary to a target site sequence in a chromosome or episome in the yeast. The RGEN can bind to, and optionally cleave, one or both DNA strands at the target site sequence. An example of an RGEN herein is a complex of a Cas9 protein with a guide RNA. A ribozyme is used in certain embodiments to provide an RNA component lacking a 5?-cap. Further disclosed are methods of genetic targeting in non-conventional yeast.
Type:
Grant
Filed:
July 5, 2015
Date of Patent:
December 24, 2019
Assignee:
DUPONT US HOLDING, LLC
Inventors:
Ryan Frisch, Xiaochun Fan, Seung-Pyo Hong
Abstract: Disclosed are methods for treating catmint oil. The treated catmint oil can be used for the production of hydrogenated catmint oil, which is enriched in the insect repellent, dihydronepetalactone.
Type:
Grant
Filed:
May 23, 2017
Date of Patent:
October 15, 2019
Assignee:
DUPONT US HOLDING, LLC
Inventors:
Scott Christopher Jackson, Leo Ernest Manzer, Henry Max Schleinitz, Mark A Scialdone, Keith W Hutchenson