Abstract: The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ?-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed.
Type:
Grant
Filed:
March 16, 2010
Date of Patent:
November 3, 2015
Assignee:
DYADIC NEDERLAND B.V.
Inventors:
Peter J. Punt, Richard Paul Burlingame, Christine M. Pynnonen, Phillip T. Olson, Jan Wery, Johannes Heinrich Visser, Mark A. Emalfarb, Jacob Visser, Jan Cornelis Verdoes, Jacoba Verdoes