Abstract: Compounds useful as labels with properties comparable to known fluorescent compounds. The compounds are conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.
Type:
Application
Filed:
August 10, 2012
Publication date:
February 21, 2013
Applicants:
Dyomics GmbH, Pierce Biotechnology, Inc.
Inventors:
Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa Dworecki, Frank G. Lehmann, Marie Christine Nlend
Abstract: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.
Type:
Application
Filed:
December 20, 2011
Publication date:
November 22, 2012
Applicants:
DYOMICS GMBH, PIERCE BIOTECHNOLOGY, INC.
Inventors:
Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa Dworecki, Frank G. Lehmann
Abstract: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.
Type:
Application
Filed:
December 20, 2011
Publication date:
June 21, 2012
Applicants:
DYOMICS GMBH, PIERCE BIOTECHNOLOGY, INC.
Inventors:
Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa Dworecki, Frank G. Lehmann
Abstract: Compounds, compositions, and methods for optical, including fluorescence optical, determinations useful in labelling biomolecules such as protein and deoxyribonucleic acid for their detection and quantitation. The compounds are diasteromeric cyanines with high hydrophilicity and other desirable properties.
Type:
Grant
Filed:
December 5, 2006
Date of Patent:
June 29, 2010
Assignee:
Dyomics GmbH
Inventors:
Peter T. Czerney, Wilhelm G. Frank, Frank G. Lehmann, Bernd G. Schweder, Matthias S. Wenzel
Abstract: The invention relates to fluorescent dyes (fluorophores) based on polymethines for use in optical measurement and detection procedures, in particular those employing fluorescence, for example in medicine, in pharmacology and in the biological, materials and environmental sciences. The objective was to create fluorophores based on polymethines that have a large Stokes shift, high photostability, long storage life and a high fluorescent quantum yield, and that can be excited in the simplest possible manner by white-light sources or laser radiation in the UV, visible or NIR spectral region. According to the invention dyes on the basis of polymethines having the general formulas I, II or III are employed.
Type:
Grant
Filed:
May 14, 2004
Date of Patent:
July 21, 2009
Assignee:
Dyomics GmBH
Inventors:
Peter Czerney, Matthias Wenzel, Bernd Schweder, Frank Lehmann
Abstract: The invention relates to compounds based on merocyanines with the general formulas I and/or II, where n=0 or an integer from 1 to 3, X=O or S and the residues R1 to R12 are the same or different and signify in particular hydrogen or various organic residues, without or with functional groups, and R2 incorporates an amine function. The compounds in accordance with the invention are suitable as fluorescent dyes (fluorophores) for used in optical determination and demonstration methods, in particular those employing fluorescence, for example in medicine, pharmacology and the biological, materials and environmental sciences. They are characterized by high photostability and long storage life, and exhibit a high fluorescence quantum yield. Excitation causing them to emit light can be achieved in a simple manner, by white-light sources or by laser irradiation in the UV, visible or NIR spectral region.
Type:
Grant
Filed:
July 3, 2002
Date of Patent:
April 1, 2008
Assignee:
Dyomics GmbH
Inventors:
Peter Czerney, Wilhelm Frank, Matthias Wenzel, Frank Lehmann
Abstract: Compounds, compositions, and methods for optical, including fluorescence optical, determinations useful in labelling biomolecules such as protein and deoxyribonucleic acid for their detection and quantitation. The compounds are diasteromeric cyanines with high hydrophilicity and other desirable properties.
Type:
Application
Filed:
December 5, 2006
Publication date:
June 7, 2007
Applicant:
DYOMICS GMBH
Inventors:
Peter Czerney, Wilhelm Frank, Frank Lehmann, Bernd Schweder, Matthias Wenzel
Abstract: The present invention relates to the field of optical-fluorescent markers, particularly to benzopyrylo-polymethine-based optical-fluorescent markers.
Type:
Grant
Filed:
December 10, 2003
Date of Patent:
August 2, 2005
Assignee:
Dyomics GmbH
Inventors:
Peter Czerney, Matthias Wenzel, Bernd Schweder, Wilhelm Frank
Abstract: The invention relates to fluorescent dyes (fluorophores) based on polymethines for use in optical measurement and detection procedures, in particular those employing fluorescence, for example in medicine, in pharmacology and in the biological, materials and environmental sciences. The objective was to create fluorophores based on polymethines that have a large Stokes shift, high photostability, long storage life and a high fluorescent quantum yield, and that can be excited in the simplest possible manner by white-light sources or laser radiation in the UV, visible or NIR spectral region.
Type:
Application
Filed:
May 14, 2004
Publication date:
December 23, 2004
Applicant:
Dyomics GmbH
Inventors:
Peter Czerney, Matthias Wenzel, Bernd Schweder, Frank Lehmann
Abstract: The invention relates to fluorescent dyes (fluorophores) based on polymethines for use in optical measurement and detection procedures, in particular those employing fluorescence, for example in medicine, in pharmacology and in the biological, materials and environmental sciences.
Type:
Application
Filed:
December 5, 2002
Publication date:
September 4, 2003
Applicant:
Dyomics GmbH
Inventors:
Peter Czerney, Matthias Wenzel, Bernd Schweder, Frank Lehmann