Abstract: A cassette for containing a gel slab used in an electrophoresis separation comprises a pair of spaced glass or like material plates having spacer strips interposed between marginal side edge areas of the plates to define a space for containing a gel slab between the plates. The spacer strips are made of a flexible double rolled smooth finished polymeric, preferably polyvinyl chloride material which when used for such purpose function to interface under pressing pressure imposed thereon to exclude atmospheric air at the interface so that once interfaced with the plates, atmospheric air pressure acting on the plates maintains a pressed sealed interface condition effective to prevent liquid flow from a liquid-containing gel slab composition cast in the cassette sideways through the cassette during gelation of the composition. An assembly tool and method for effecting cassette assembly is provided.

Abstract: The present invention is directed to an apparatus for electro elution of components separated by preparative electrophoresis on a gel comprising a tapered tube divided by a porous disc into an upper section which is open at the top of the tapered tube and a lower section which is closable by a removable cap. The apparatus also contains a dialysis membrane of substantially the same diameter as the removable cap and affixed to the removable cap such that the dialysis membrane is sealed against the end of the tapered tube when the removable cap encloses the lower section of the tapered tube. Following electro elution, the open upper section of the tapered tube can be sealed and the desired substance is withdrawn through the cap and dialysis membrane which encloses the lower end of the tube. An auxiliary funnel can be affixed to the open upper end of the tube to increase the capacity of the system.

Abstract: An improved field-test method and apparatus for the detection of asbestos by detecting magnesium or iron is described wherein all of the reactions of the test are performed in one reaction tube or column. The method includes the steps of placing a sample in the tube, washing the sample in the tube, adding acid reagent to the sample, and adding a color reagent to the sample in the tube to indicate qualitatively if asbestos is present in the sample. The apparatus is in the form of a compact kit containing multiple columns with caps and other labware needed for the test.

Abstract: Vertical gel electrophoresis apparatus of the type wherein the gel is cast in a horizontal position using an extending top wall to define an end of the casting chamber, is improved by making the top wall removable to allow access to sample slots in the gel from directly overhead and to provide for attachment of a syringe holder at the position of the removed wall.

Abstract: A counter-double-current device having an array of interconnected tube elements, each of the elements having a mixing tube, a light phase decant tube and an intermediate phase decant tube. The light and intermediate phase decant tubes are disposed parallel to the mixing tube with the intermediate phase decant tube positioned between the light phase decant tube and the mixing tube. A first juncture tube joins the mixing tube and light phase decant tube while a second juncture tube joins the light and intermediate phase decant tubes. First and second downspout tubes are sealably mounted within the lower portions of the two decant tubes.

Abstract: A device for mixing immiscible or semi-immiscible liquids and subsequently separating one or more of them followed by transfer is disclosed. An array of elements is utilized with the center decant transfer tube of one element connecting with the mixing tube of the adjacent element to the right. This is used for transfer of the upper phase material, generally known as the effluent phase. Transfer of the lower phase or the residual phase is accomplished by transferring to the mixing tube indicated to the left in the array. The upper phase is first decanted through a center decant tube, and then, prior to mixing, the lower phase is transferred. The sequence of transfer can be reversed if the units are stacked so that movement proceeds in opposite directions in adjacent rows of elements.

Abstract: A multiple cell electrophoresis elution device is disclosed. Gels are fractionated to locate, by scanning, the component of interest and the gel is sliced to isolate this band. In one embodiment, it is then placed in one compartment of the elution device and recast in place using a dilute gel. This recasting mechanically holds the gel and acts as a separating barrier from one buffer compartment to another. The gel casting procedure prevents both dilution of the eluted fractions by mixing due to mechanical or convective influences as well as insuring that the current must pass through the gel and not around it in the more conductive free buffer. In another embodiment, the cut gel is covered with an element to occupy the remaining volume of the gel compartment and compress the gel.The device employs a series of compartments joined to a single set of electrodes such that a number of distinct separate fractions may be eluted simultaneously.

Abstract: A process of separating and isolating fractions containing isoenzymes from a sample of tissue extract or blood serum comprising ion-exchange column chromatographically separating the sample containing the isoenzymes into a first fraction containing the hepatic isoenzyme of lactic acid dehydrogenase and a second fraction containing the myocardial isoenzymes of lactic acid dehydrogenase, the myocardial isoenzyme of creatine phosphokinase and prostatic acid phosphatase by ion-exchange column chromatographing the sample on a weakly-basic anion exchange chromatographic resin and sequentially eluting and collecting the first fraction and the second fraction.