Abstract: Method and apparatus for collecting and/or growing anaerobic or aerobic biological specimens. A slidable inner rod and outer tube assembly is provided with rear, middle and front seals which define two coaxial sealed chambers of variable volume within the rod and tube assembly. The front chamber is controllably unsealed to receive a biological specimen. The front chamber is then resealed by manipulation of the tube and rod assembly which may also thereafter cause passage of a biological culture growth medium, transport materials, etc. from a rear chamber into the front chamber by reducing the volume of the rear chamber. The biological specimen is thus collected and may be transferred to a growth medium or the like in a completely protected environment immediately after its collection within an internal organ (for example) of a living body.