Abstract: A method is provided for converting heat from a heat source to mechanical energy. The method comprises heating a working fluid using heat supplied from the heat source; and expanding the heated working fluid to lower the pressure of the working fluid and generate mechanical energy as the pressure of the working fluid is lowered. The method is characterized by using a working fluid comprising HFC-245eb and optionally Z-HFO-1336mzz. A power cycle apparatus containing a working fluid to convert heat to mechanical energy is also provided. The apparatus is characterized by containing a working fluid comprising HFC-245eb and optionally Z-HFO-1336mzz. A working fluid comprising HFC-245eb and optionally Z-HFO-1336mzz is also provided. The working fluid (i) further comprises E-HFO-1336mzz, (ii) has a temperature above its critical temperature, or both (i) and (ii).

Abstract: Methods are provided for pelletizing a microbial biomass, extracting a refined lipid composition from the pelletized biomass under supercritical conditions and distilling the refined lipid composition, at least once under short path distillation conditions, to obtain a lipid-containing fraction. Also disclosed are methods of making lipid-containing oil concentrates therefrom, by transesterifying and enriching the lipid-containing fraction.

Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.

Abstract: Disclosed is a method for detecting a leak in a closed loop heat transfer system comprising monitoring the pressure of the heat transfer composition inside said heat transfer system, wherein a drop in pressure indicates a leak. Also disclosed is a heat transfer system comprising an evaporator, a compressor, a condenser, an expander and a device for measuring internal system pressure. The system pressure measuring device is disposed inside the closed loop heat system. The internal system pressure measuring means may be located either between the evaporator and the condenser, between the expander and the evaporator, between the compressor and the condenser, or between the condenser and the expander.

Abstract: The invention relates to the use of and method of forming Low Temperature Cofired Ceramic (LTCC) circuits for high frequency applications. Furthermore, the invention relates to the novel LTCC thick film compositions and the structure itself.

Abstract: Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate/ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.