Abstract: Provided are a fluorescent probe and a preparation process and the use thereof. The fluorescent probe is sensitive and specific to viscosity, and can be used for specific fluorescent labeling of proteins, and can also be used for quantification, detection or kinetic studies of proteins, and the imaging of cells, tissues and living bodies.

Abstract: The invention relates to a preparation method of green nylon poly butyrolactams, the biological materials of GABA in vacuum under the condition of high temperature melt decomposition and purified butyrolactams, then by vacuum polymerization of green nylon poly butyrolactams. Compared with the prior art, the invention is prepared by biological method for the synthesis of a wide range of sources, to solve the problem of raw material supply PA4 for mass production, reduce the cost of reaction, and the reaction condition is simple, easy to implement simplified synthesis steps, from the laboratory to the transformation of industrial production.

Abstract: A method for preparation of a high-viscosity polyester hot-melt adhesive with a partially sensitive viscosity-temperature property is disclosed, including the steps of: i) subjecting terephthalic acid, isophthalic acid, dodecanedioic acid, adipic acid, butanediol, dipropylene glycol and hexanediol to an esterification reaction in the presence of tetrabutyl titanate serving as a catalyst; and ii) adding an antioxidant to a product resulting from step i) to conduct a polycondensation reaction under a reduced pressure so as to obtain the high-viscosity polyester hot-melt adhesive which has a low melting point of 130° C.-135° C. and a partially sensitive viscosity-temperature property.

Abstract: Disclosed herein is Candida parapsilosis CGMCC 9630, the carbonyl reductase expressed by said strain and the encoding gene and amino acid sequence thereof, the recombinant expression vector and recombinant expression transformant containing said gene sequence, and use of whole cells of Candida parapsilosis, carbonyl reductase or corresponding recombinant transformant thereof as catalyst in catalyzing asymmetric reduction of prochiral carbonyl compounds, particularly reduction of 6-carbonyl-8-halogenocaprylate to prepare the synthetic precursor of (R)-?-lipoic acid, (R)-6-hydroxy-8-halogenocaprylate. In comparison to other methods of asymmetric reduction for preparing (R)-6-hydroxy-8-halogenocaprylate, the disclosure has advantages of high substrate concentration, mild reaction conditions, environmental friendship, high yield, and high optical purity of the product, and thus has good prospect in industrial production of (R)-?-?-lipoic acid.

Abstract: A photo-tunable liquid crystal composition may include a polymer matrix and a liquid crystal mixture dispersed within the polymer matrix. The liquid crystal mixture may include at least one achiral nematic liquid crystal material, at least sine photo-active chiral dopant, at least one photo-inactive chiral dopant, and at least one emissive dye. The composition may be fabricated in the form of an emulsion, a wet film, or a dry film. The dry film may be used in a photo-tunable liquid crystal film laser. The output wavelength of the photo-tunable liquid crystal film laser may be tuned by exposing the dry film to illumination having at least one wavelength at one or more transition wavelengths.

Abstract: Provided is an optically controlled gene expression system of prokaryotic bacterium, comprising: a) a photosensitive recombinant transcription factor encoding gene, the photosensitive recombinant transcription factor is one fusion protein comprising a first polypeptide as the DNA bonding domain and a second polypeptide as the photosensitive domain; b) a target transcription unit comprising promoter or promoter-reaction element or reaction element-promoter containing at least one reaction element recognized/bound by the first polypeptide and the nucleic acid sequence to be transcribed. Also provided is a prokaryotic expression vector comprising said optically controlled gene expression system, and a method for regulating gene expression in a prokaryotic host cell by using the optically controlled gene expression system. Also provided is a reagent kit containing different components of the optically controlled gene expression system.

Abstract: A method and a device for synchronous high-speed photographing of microparticle rotation in a liquid cyclone field and for determining the rotation velocity of a microparticle in a liquid cyclone field by using a combination of a synchronous high-speed photographing system and a transparent microparticle containing two centrosymmetrically arranged inner cores having the same diameter. The method comprises: using a transparent microparticle comprising two inner cores having the same diameter and arranged centrosymmetrically as a rotation test particle; acquiring synchronously two groups of two dimensional image series of microparticle motion in a liquid cyclone field using two orthogonally arranged high-speed digital cameras; and reconstructing a three dimensional motion trajectory of the microparticle from the two groups of synchronous image series, and determining a rotation velocity of the microparticle in the cyclone field at the same time.

Abstract: The invention relates to a design method of high-temperature nickel-based bolts based on damage tolerance theory, comprising the following steps: S1: acquiring operating parameters for the design; S2: selecting a material for bolts; S3: acquiring mechanical properties of the materials; S4: determining a pretension stress ?p of a single bolt; S5: determining the service stress ?s under the steady state; S6: determining the number n, the effective cross-section area A and the distribution of bolts; S7: determining a maximum allowable crack dimension; S8: calculating the maximum allowable service stress ?th using the crack propagation threshold Kth at the design temperature; S9: comparing the service stress ?s and the maximum allowable service stress ?th, if ?s is smaller than ?th, then the bolts are safe in the design life; otherwise, return to step S4 and reduce the pretension stress ?p.

Abstract: Methods and catalyst compositions for oxidizing CO to CO2 at low temperatures are disclosed. In one embodiment, a method of oxidizing CO to CO2 may involve heating a gaseous mixture comprising at least CO and O2 with a catalyst mixture comprising Pd disposed on a Mn3O4 mesoporous support at a temperature of about 0° C. to about 60° C., and wherein the CO to CO2 conversion rate is about 40% to about 100%.

Abstract: Implementations and techniques for preparing and using monomers, oligomeric complexes, and coordination polymers are generally disclosed.

Abstract: Methods described herein generally relate to producing carbon aerogel. The method may include providing a carbon-containing polymeric material, and contacting the carbon-containing polymeric material with light, heat or both to produce the carbon aerogel. Systems and kits for producing carbon aerogel are also disclosed.

Abstract: The present invention relates to a supported polymetal olefin polymerization catalyst, comprising a porous support, a magnesium-containing support component, a transition metal titanium component supported on the porous support, and further comprising at least one non-magnesium metal component supported on the porous support. Further provided is a preparation method and a use of the supported polymetal olefin polymerization catalyst. An efficient composite support supported polymetal Ziegler-Natta catalyst is provided in the present invention, wherein a porous support, a soluble magnesium compound, and a soluble non-magnesium metal compound are used as raw materials. The supporting of titanium is achieved while a composite support containing magnesium and non-magnesium metal components is formed in situ in the surface of the porous support. The present invention has the advantage of a simple preparation method, a low cost, a controllability of morphology, properties of the catalyst, etc.

Abstract: Provided is an electric desalting and dehydration apparatus, including an oil inlet distributor of an upper electric field, wherein the oil inlet distributor includes an oil inlet distributing pipe and an oil inlet branch pipe. The oil inlet distributing pipe is arranged in a water tray of an electric desalter, and below a ground electrode of the electric desalter. The oil inlet branch pipe is connected to the oil inlet distributing pipe to supply crude oil into the oil inlet distributing pipe. The oil inlet distributing pipe is provided with oil outlets on both sides thereof in a horizontal direction. The distributor can uniformly distribute crude oil in a water tray, thus all of the crude oil is kept in an electric field for an equal time.

Abstract: Methods of making metal oxide-graphene composites are disclosed. The methods can include, for example, providing a composition including graphene oxide and at least one substrate, the composition being dispersed in a liquid medium. The methods can also include, for example, providing a composition including graphene oxide and at least one substrate, heating the composition, and cooling the composition. Compositions useful for performing the methods and composites obtained by the process are also disclosed.

Abstract: Methods, systems and kits for preparing carbamates as well as catalysts for preparing the carbamates, are disclosed. The methods for preparing carbamate can include providing a catalyst comprising cerium oxide (CeO2) and at least one metal selected from the group consisting of iron (Fe), manganese (Mn), titanium (Ti), cobalt (Co), aluminum (Al), zinc (Zn), calcium (Ca), tin (Sn), indium (In), and any combination thereof; contacting the catalyst with at least one amine and at least one alcohol to form a mixture; and contacting the mixture with carbon dioxide under conditions sufficient to form the carbamate.

Abstract: A dual frequency liquid crystal may be stabilized in the blue phase by a polymer matrix to provide an improved blue phase temperature range having a magnitude of at least about 65° C. Polymer-stabilized, blue phase dual frequency liquid crystal compositions and methods for producing polymer-stabilized, blue phase dual frequency liquid crystals are disclosed.

Abstract: The invention relates to a genetically encoded fluorescent sensor for nicotinamide adenine dinucleotide, as well as methods of preparation and uses thereof. In one aspect, this invention relates to a sensor for detecting nicotinamide adenine dinucleotide, particularly, a recombinant fluorescent fusion protein sensor for detecting nicotinamide adenine dinucleotide. In one specific aspect, this invention relates to a recombinant fluorescent fusion protein sensor for detecting reduced nicotinamide adenine dinucleotide (NADH); in another specific aspect, this invention relates to a recombinant fluorescent fusion protein sensor for detecting oxidized nicotinamide adenine dinucleotide (NAD+); in yet another aspect, the invention relates to a recombinant fluorescent fusion protein sensor for detecting the ratio of reduced to oxidized nicotinamide adenine dinucleotide.

Abstract: Disclosed is a method of measurement and determination on fracture toughness of structural materials at high temperature, comprising: preliminary assessing the ductility of a material based on a high-temperature uniaxial tensile test and the fracture characteristic; designing and manufacturing a CT specimen; conducting a monotonic loading fracture test on the CT specimen at high temperature; modifying a load-displacement curve output by a testing machine; determining a passivation coefficient M for the crack of the structural material; reversely recursing instant load-displacement data pairs corresponding to the instant crack length; calculating a J_R crack extension resistance curve of the tensile test; examining the validity of the J_R crack extension resistance curve and the fracture toughness JIC; calculating the fracture toughness per equivalent of the structural material KIC. The present invention overcomes the difficulty of placing an extensometer inside a high-temperature furnace.

Abstract: Methods described herein generally relate to preparing graphene. The method may include contacting at least one elemental metal with a composition having graphene oxide under conditions sufficient to reduce at least a portion of the graphene oxide to graphene. Systems and kits for preparing graphene are also disclosed.

Abstract: Biopesticide compositions, and methods and kits for their preparation from lignin are disclosed. A biopesticide may include a modified lignin chelated with one or more pesticides, the one or more pesticides having at least one acidic pesticide. The modified lignin may have one or more functional groups. Methods of producing a biopesticide may include obtaining a lignin, contacting the lignin with one or more bacteria under conditions suitable for producing a modified lignin, and chelating the modified lignin with one or more pesticides, the one or more pesticides having at least one acidic pesticide.