Abstract: Real time biofilm monitoring systems are provided. Said systems comprise single or multiple fiber-optic probes detecting wavelength-specific fluorescence from biomarkers of fouling organisms; a compact optoelectronic interface and data acquisition system interfaced with said probes, wherein said probe or probes are bifurcated and contain at least one excitation and at least one emission filter permitting the simultaneous resolution of multiple biomarkers.

Abstract: Real time biofilm monitoring systems are provided. Said systems comprise single or multiple fiber-optic probes detecting wavelength-specific fluorescence from biomarkers of fouling organisms; a compact optoelectronic interface and data acquisition system interfaced with said probes, wherein said probe or probes are bifurcated and contain at least one excitation and at least one emission filter permitting the simultaneous resolution of multiple biomarkers.

Abstract: Bacterial spores can be rapidly and sensitively detected and quantified based upon molecular recognition of unique chemicals in the spore coat. Spores can be detected and assayed based upon the calcium concentration in bacterial spore coats using the calcium which is unique to the bacterial spores. Since spores contain a high concentration of calcium relative to other biological materials, fluorescent calcium-sensitive indicators are used to detect Ca+2 displaced from the spore case or free in solution or from the aerosol phase. Visibly excitable fluorescent dyes provide a sensitive and selective means to monitor changes in spore concentration and avoid difficulties associated with laser or UV-excitation.