Abstract: A process for enzymatically converting a furanoside substrate in a product of interest, includes contacting the substrate with an enzyme in presence of an alcohol acceptor, wherein the enzyme is preferably Araf51, and wherein the product is preferably an alkyl furanoside. The mutant Araf51 enzyme showing improved transglycosylation activity in comparison with the native wild-type (wt) Araf51 enzyme, and a method for screening the mutants are also described.
Type:
Application
Filed:
April 19, 2013
Publication date:
May 7, 2015
Applicants:
ECOLE NATIONALE SUPERIEURE DE CHIMIE DE RENNES (ENSCR), CENTRE NATIOANAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
Inventors:
Richard Daniellou, Caroline Nugier-Chauvin, Vincent Ferrieres, Alize Pennec, Ilona Chlubnova