Abstract: A nonlinear optical microscope is provided, including source of a pulsed laser beam; a spatial light modulator for modulating the spatial profile of the pulsed laser beam; an objective for guiding the modulated beam towards a slide intended to carry a specimen; and a detector for collecting signals originating from the specimen, wherein the spatial light modulator is designed to modulate the intensity and/or the phase of the pulsed laser beam on the rear pupil of the objective to produce a beam that is axially extended and confined in one or two lateral directions after focusing by the objective, and wherein the slide is placed on a motorized stage of a histology slide scanner assembly.
Type:
Application
Filed:
December 3, 2012
Publication date:
November 27, 2014
Applicants:
ECOLEE POLYECHNIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Inventors:
Marie-Claire Schanne-Klein, Emmanuel Jean-Marc Beaurepaire, Mathias Strupler, Delphine Debarre, Nicolas Olivier, Pierre Mahou