Patents Assigned to EdeniQ, Inc.
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Patent number: 11913060Abstract: Methods and solutions are disclosed for the quantification of mixed-linkage beta-glucan in plant biomass samples. Improved alkaline pretreatment, enzymatic hydrolysis, and glucose measurement parameters are provided that increase the accuracy, precision, and sensitivity of mixed-linkage beta-glucan assays.Type: GrantFiled: December 20, 2019Date of Patent: February 27, 2024Assignee: Edeniq, Inc.Inventors: Prachand Shrestha, Denmark Antolin
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Publication number: 20230104032Abstract: The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatment. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.Type: ApplicationFiled: September 30, 2022Publication date: April 6, 2023Applicant: Edeniq, Inc.Inventors: Deepak Singh, Sandra Jacobson, Kris Ramos, Prachand Shrestha
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Patent number: 11492649Abstract: The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatement. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.Type: GrantFiled: March 21, 2019Date of Patent: November 8, 2022Assignee: Edeniq, Inc.Inventors: Deepak Singh, Sandra Jacobson, Kris Ramos, Prachand Shrestha
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Publication number: 20210246486Abstract: Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.Type: ApplicationFiled: February 22, 2021Publication date: August 12, 2021Applicant: EdeniQ, Inc.Inventors: David Scott Snyder, Prachand Shrestha
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Patent number: 10954547Abstract: Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.Type: GrantFiled: December 21, 2018Date of Patent: March 23, 2021Assignee: EDENIQ, INC.Inventors: David Scott Snyder, Prachand Shrestha
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Patent number: 10767204Abstract: The present disclosure provides methods and systems for generating sugars from biomass. The methods and systems provide increased yields of carbohydrates and fermentable sugars by combining enzyme recycling with removal of saccharification enzyme inhibitors and increased solids loading.Type: GrantFiled: December 20, 2013Date of Patent: September 8, 2020Assignee: Edeniq, Inc.Inventors: James Kacmar, Rich Kleinke, Bernard Cooker, Chris Kalinowski, Jon Van Volkinburg
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Patent number: 10501765Abstract: This disclosure provides a high solids biomass slurry that is readily pumpable and transportable to downstream processing units, such as chemical and/or biochemical processing units. The slurry is amenable to saccharification efficiencies of >70 % in processing times of <36 hours. Also provided are devices for processing materials, such as the high solids biomass slurry.Type: GrantFiled: March 25, 2016Date of Patent: December 10, 2019Assignee: Edeniq, Inc.Inventors: Jeffrey R. Charron, Thomas P. Griffin
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Patent number: 10260081Abstract: The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatment. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.Type: GrantFiled: March 14, 2014Date of Patent: April 16, 2019Assignee: Edeniq, Inc.Inventors: Deepak Singh, Sandra Jacobson, Kris Ramos, Prachand Shrestha
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Patent number: 10077436Abstract: Described herein are beta-glucosidase enzymes that have improved beta-glucosidase activity compared to a control beta-glucosidase enzyme. The improved beta-glucosidase enzymes are useful for converting a cellulosic biomass to fermentable sugars such as glucose. Also described are isolated polynucleotides that encode polypeptides having improved beta-glucosidase activity, expression cassettes for expressing the improved beta-glucosidase polypeptides, and cells, such as yeast cells, transformed with the expression cassettes.Type: GrantFiled: September 16, 2014Date of Patent: September 18, 2018Assignee: EDENIQ, INC.Inventors: Lan Sun, Ngim Muy Ung Ferrell, Deepak Singh, Jennifer Headman, Mei-Hua Chu, Michael Guerini, Sandra Jacobson
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Publication number: 20150344921Abstract: The present disclosure provides methods and systems for generating sugars from biomass. The methods and systems provide increased yields of carbohydrates and fermentable sugars by combining enzyme recycling with removal of saccharification enzyme inhibitors and increased solids loading.Type: ApplicationFiled: December 20, 2013Publication date: December 3, 2015Applicant: Edeniq, Inc.Inventors: James KACMAR, Rich KLEINKE, Bernard COOKER, Chris KALINOWSKI, Jon VAN VOLKINBURG
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Patent number: 9169498Abstract: Processes and methods of recovering desired products from fermentation stillage are presented, including processes and methods of recovering lipids and aqueous materials.Type: GrantFiled: November 4, 2013Date of Patent: October 27, 2015Assignee: Edeniq, Inc.Inventors: Richard Root Woods, Vahik Krikorian, Juston Smithers
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Patent number: 9085781Abstract: Improved haa1 transcriptional regulatory proteins, polynucleotides encoding improved haa1 transcriptional regulatory proteins and vectors and cells thereof are provided, as well as methods for converting a cellulose-containing biomass feedstock to ethanol using improved haa1 transcriptional regulatory proteins and cells expressing heterologous haa1 transcriptional regulatory proteins as disclosed herein.Type: GrantFiled: July 22, 2013Date of Patent: July 21, 2015Assignee: Edeniq, Inc.Inventors: Kenneth Zahn, Sandra Jacobson
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Publication number: 20140206055Abstract: The present disclosure provides methods and compositions for reducing the viscosity of biomass process streams in an ethanol production process. The method comprises adding cellulase enzymes to a biomass feedstock that is fermented to produce ethanol, generating whole stillage and thin stillage streams from the post-fermentation biomass, and adding an additional enzyme or enzyme cocktail that reduces the viscosity of the whole stillage stream, thin stillage stream, concentrated thin stillage stream, and/or the syrup stream generated by evaporating the thin stillage.Type: ApplicationFiled: January 24, 2014Publication date: July 24, 2014Applicant: Edeniq, Inc.Inventors: Kristoffer Ramos, Donna Santos, Padmavathy Desai, Prachand Shrestha, Richard Root Woods
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Publication number: 20140178946Abstract: This disclosure provides for materials and methods for converting biomass to biofuels. The materials include a colloid mill with or without cellulase enzymes, and the methods include the use of a colloid mill and optionally cellulose enzymes to pretreat biomass for use in a biomass to biofuel production process.Type: ApplicationFiled: February 25, 2014Publication date: June 26, 2014Applicant: Edeniq, Inc.Inventors: Adriano Galvez, III, Glenn Richards
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Patent number: 8686123Abstract: Improved manganese peroxidases, polynucleotides encoding improved manganese peroxidase and vectors and cells thereof are provided, as well as methods for converting a cellulose-containing biomass feedstock to ethanol using improved manganese peroxidases and cells expressing a heterologous manganese peroxidase as disclosed herein.Type: GrantFiled: November 15, 2011Date of Patent: April 1, 2014Assignee: Edeniq, Inc.Inventors: Chris DaCunha, Adrian Galvez, Michael Guerini, Manoj Patel, Ngim Ung-Ferrell, Jennifer Headman
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Publication number: 20140024084Abstract: This disclosure provides for materials and methods for converting biomass to biofuels. The materials include a colloid mill with or without cellulase enzymes, and the methods include the use of a colloid mill and optionally cellulose enzymes to pretreat biomass for use in a biomass to biofuel production process.Type: ApplicationFiled: September 27, 2013Publication date: January 23, 2014Applicant: EdeniQ, Inc.Inventors: Adriano Galvez, III, Glenn Richards
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Publication number: 20140024095Abstract: Improved haa1 transcriptional regulatory proteins, polynucleotides encoding improved haa1 transcriptional regulatory proteins and vectors and cells thereof are provided, as well as methods for converting a cellulose-containing biomass feedstock to ethanol using improved haa1 transcriptional regulatory proteins and cells expressing heterologous haa1 transcriptional regulatory proteins as disclosed herein.Type: ApplicationFiled: July 22, 2013Publication date: January 23, 2014Applicant: EdeniQ, Inc. (027586)Inventors: Kenneth Zahn, Sandra Jacobson
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Publication number: 20140017732Abstract: The present application describes a dilute two-loop method for treating biomass in the production of biofuels such as ethanol. The method involves washing the biomass to dissolve sugars that are associated with the biomass, and separating the washed biomass into a solids phase and a liquids phase. The solids phase is pretreated to render the biomass more susceptible to hydrolysis under conditions that do not produce substantial amounts of sugars. The pretreated biomass is separated into a second solids phase and second liquid phase, and the second solids phase is saccharified and fermented. The first and second liquid phases are recycled to dilute the biomass at various stages of the process.Type: ApplicationFiled: March 15, 2013Publication date: January 16, 2014Applicant: Edeniq, Inc.Inventor: Edeniq, Inc.
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Patent number: 8609874Abstract: Processes and methods of recovering desired products from fermentation stillage are presented, including processes and methods of recovering lipids and aqueous materials.Type: GrantFiled: July 11, 2012Date of Patent: December 17, 2013Assignee: Edeniq, Inc.Inventors: Richard Root Woods, Vahik Krikorian, Juston Smithers
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Patent number: 8563272Abstract: The present invention provides for combinations of enzymes and other proteins that result in improved saccharification of plant material. The invention provides for saccharification in the presence of and optional fermentation by, yeast cells expressing the enzymes and other proteins.Type: GrantFiled: June 26, 2009Date of Patent: October 22, 2013Assignee: Edeniq, Inc.Inventors: Adriano Galvez, III, Glenn Richards