Abstract: The mutation of the endA and recA genes in E. coli strain BL21 creates features that are very attractive for protein expression and cloning in a single prokaryotic system, including rapid cell growth, high transformation efficiency, rapid plasmid replication to high densities, and high plasmid stability. When specially prepared for competence, these strains are highly useful for both cloning and protein expression.

Abstract: A method and materials are described for purification of DNA using ion exchange resins. This ion exchange method provides the means to remove salts and low molecular weight contaminants from DNA in solution without binding or removing the DNA of interest.

Abstract: Compositions and methods are provided for adhering and binding biologically active proteins and protein-containing composites to substrates. Adhesive formulations comprising a nonproteinaceous polymer of monomeric units comprising an aromatic moiety substituted with at least one hydroxyl group such as poly(p-hydroxy-styrene) are applied to substrates and subsequently contacted with proteins. Beads comprising a nonproteinaceous polymer of monomeric units comprising an aromatic moiety substituted with at least one hydroxyl group are also provided, and the beads are coated with a protein. Substrates to which the adhesive formulations have been applied, as well as the beads, can be used to adhere cells and tissues, to sort cell types, to perform immunoassays, to perform chromatography and to remove protein from samples.