Abstract: A method and materials are described for purification of DNA using ion exchange resins. This ion exchange method provides the means to remove salts and low molecular weight contaminants from DNA in solution without binding or removing the DNA of interest.
Type:
Grant
Filed:
July 5, 2001
Date of Patent:
January 7, 2003
Assignee:
Edge Biosystems, Inc.
Inventors:
Eva Kristyanne, Reyes Candau, John L. Seed
Abstract: Compositions and methods are provided for adhering and binding biologically active proteins and protein-containing composites to substrates. Adhesive formulations comprising a nonproteinaceous polymer of monomeric units comprising an aromatic moiety substituted with at least one hydroxyl group such as poly(p-hydroxy-styrene) are applied to substrates and subsequently contacted with proteins. Beads comprising a nonproteinaceous polymer of monomeric units comprising an aromatic moiety substituted with at least one hydroxyl group are also provided, and the beads are coated with a protein. Substrates to which the adhesive formulations have been applied, as well as the beads, can be used to adhere cells and tissues, to sort cell types, to perform immunoassays, to perform chromatography and to remove protein from samples.