Patents Assigned to Edward E. Winger
  • Real time homogeneous nucleotide assay
    Patent number: 5853990
    Abstract: A method for performing real time, homogeneous assay of a target nucleic acid comprising annealing a labeled, ribo-oligonucleotide probe to the target DNA sequence and degrading a portion of the probe with RNase H to release labeled fragments. Sequential measurements of the released fragments permits kinetic characterization of the presence of the target sequence. Preferably, the assay is integrated into a polymerase chain reaction so that target amplification can be detected in real time.
    Type: Grant
    Filed: July 26, 1996
    Date of Patent: December 29, 1998
    Assignee: Edward E. Winger
    Inventors: Edward E. Winger, Donald J. Kessler, David E. Hargrove