Patents Assigned to Eiken Chemical Co., Ltd.
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Patent number: 8445719Abstract: A method of stabilizing a hem protein which is effective against the denaturation and degradation of a hem protein typified by hemoglobin and a storage solution therefor. A method of stabilizing a hem protein and a storage solution therefor characterized in that an iminocarboxylic acid or its salt is made to coexist in a sample containing the hem protein, wherein the above-described iminocarboxylic acid is a compound represented by the following general formula (1) wherein R represents a hydrogen atom or a hydroxyl group; and X's represent each a hydrogen atom, an alkali metal or an ammonium group.Type: GrantFiled: September 12, 2012Date of Patent: May 21, 2013Assignee: Eiken Chemical Co., Ltd.Inventor: Shin Sugo
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Publication number: 20130004975Abstract: A method of stabilizing a hem protein which is effective against the denaturation and degradation of a hem protein typified by hemoglobin and a storage solution therefor. A method of stabilizing a hem protein and a storage solution therefor characterized in that an iminocarboxylic acid or its salt is made to coexist in a sample containing the hem protein, wherein the above-described iminocarboxylic acid is a compound represented by the following general formula (1) wherein R represents a hydrogen atom or a hydroxyl group; and X's represent each a hydrogen atom, an alkali metal or an ammonium group.Type: ApplicationFiled: September 12, 2012Publication date: January 3, 2013Applicant: EIKEN CHEMICAL CO., LTD.Inventor: Shin SUGO
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Patent number: 8329943Abstract: A method of stabilizing a hem protein which is effective against the denaturation and degradation of a hem protein typified by hemoglobin and a storage solution therefor. A method of stabilizing a hem protein and a storage solution therefor characterized in that an iminocarboxylic acid or its salt is made to coexist in a sample containing the hem protein, wherein the above-described iminocarboxylic acid is a compound represented by the following general formula (1) wherein R represents a hydrogen atom or a hydroxyl group; and X's represent each a hydrogen atom, an alkali metal or an ammonium group.Type: GrantFiled: October 6, 2008Date of Patent: December 11, 2012Assignee: Eiken Chemical Co., Ltd.Inventor: Shin Sugo
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Publication number: 20100216178Abstract: A method of stabilizing a hem protein which is effective against the denaturation and degradation of a hem protein typified by hemoglobin and a storage solution therefor. A method of stabilizing a hem protein and a storage solution therefor characterized in that an iminocarboxylic acid or its salt is made to coexist in a sample containing the hem protein, wherein the above-described iminocarboxylic acid is a compound represented by the following general formula (1) wherein R represents a hydrogen atom or a hydroxyl group; and X's represent each a hydrogen atom, an alkali metal or an ammonium group.Type: ApplicationFiled: October 6, 2008Publication date: August 26, 2010Applicant: EIKEN CHEMICAL CO., LTD.Inventor: Shin Sugo
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Patent number: 5849487Abstract: The present invention relates to a method of detecting a polynucleotide, comprising hybridizing a polynucleotide of known nucleotide sequence with a nuclease-resistant oligonucleotide primer having a sequence complementary to a part of the polynucleotide, then adding at least one kind of deoxynucleoside triphosphate, DNA polymerase and nuclease thereto, synthesizing a complementary strand being a nucleotide species located adjacent to the 3'-terminal of the primer and complementary to the polynucleotide, followed by decomposition thereof, the synthesis and decomposition of the complementary strand being repeated one or more times, and detecting the resulting pyrophosphoric acid or deoxynucleoside monophosphate. The present invention also includes a detection kit used for this method of detecting a polynucleotide.Type: GrantFiled: January 29, 1996Date of Patent: December 15, 1998Assignees: Eiken Chemical Co., Ltd., Tanabe Seiyaku Co., Ltd.Inventors: Tetsu Hase, Harumi Masubuchi
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Patent number: 5719050Abstract: The present invention relates to a medium composition for culturing animal cells which is obtained by combining at least one component selected from the group of substances mentioned below with a medium composition comprising inorganic salts, saccharides, vitamins and amino acids; a method for culturing animal cells comprising adding to a medium for the cells as a cell growth promoting substance at least one component selected from the group mentioned below; a method for enhancing the antibody production of antibody-producing cells comprising adding to a medium for the cells at least one component selected from the group mentioned below; a composition for enhancing the antibody production of antibody-producing cells which is obtained by combining at least one antibody production enhancing agent selected from the group mentioned below with a composition comprising inorganic salts, saccharides, vitamins and amino acids; and a method for producing a physiologically active substance comprising culturing animal ceType: GrantFiled: December 16, 1994Date of Patent: February 17, 1998Assignee: Eiken Chemical Co., Ltd.Inventors: Makoto Hashimoto, Tsutomu Naito
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Patent number: 4378438Abstract: A tray for identifying isolated microorganisms consisting of a plastic or glass tray body and a transparent cover, the body surface of which has two kinds of hollows for bacterial suspensions. One is formed horizontally across the surface of the tray and the other is composed of number of small wells long and slender, put parallel and rectangular to previous hollow. Each of the small cells communicates over crest barrier to hollow put horizontally. With this tray, microorganisms can be exactly and easily identified.Type: GrantFiled: October 29, 1981Date of Patent: March 29, 1983Assignee: Eiken Chemical Co., Ltd.Inventors: Toshikatsu Masaki, Seiji Enomoto, Michiya Kimura
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Patent number: 4370419Abstract: A tray for identifying isolated microorganisms, comprising a plastic or glass tray body and a cover, the body surface of which has two kinds of hollow cells for bacterial suspension. One is formed horizontally across the surface of the tray and the other is composed of a number of long and slender, parallel small cells. Each of the small cells communicates over a crest barrier with another hollow cell. Each crest barrier is formed so that its top is lower in height than the upper surface of the partition wall of the tray body. With this tray, microorganisms can be exactly and easily identified by improving the accuracy of uniformly pouring bacterial suspension into respective cells for bacterial suspension.Type: GrantFiled: July 15, 1981Date of Patent: January 25, 1983Assignee: Eiken Chemical Co., Ltd.Inventors: Tamotsu Iida, Seiji Enomoto, Michiya Kimura
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Patent number: 4306019Abstract: A method is disclosed for differential estimation of glutamic acid-oxalacetic acid transaminase isozyme which comprises estimating m-GOT by: preparing an immuno conjugate by adding an anti-s-GOT obtained by immunizing a mammal with purified s-GOT as antigen extracted from the blood or the tissue of the mammal into the human serum; adding sensitized particles which are allowed to couple said anti-s-GOT antibody to insoluble particles; and completely absorbing said s-GOT in said human serum.Type: GrantFiled: February 9, 1977Date of Patent: December 15, 1981Assignee: Eiken Chemical Co., Ltd.Inventors: Hiroko Wada, Hiroyasu Teranishi