Patents Assigned to Elitech Holdings, B.V.
  • TM leveling methods
    Patent number: 7751982
    Abstract: Methods for designing an oligonucleotide sequence having a selected duplex stability are provided wherein the oligonucleotide portion comprises at least one modified base selected from universal bases, unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally having attached minor groove binders and reporter groups.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: July 6, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Robert O. Dempcy, Alexander A. Gall, Sergey G. Lokhov, Irina A. Afonina, Igor V. Kutyavin, Nicolaas M. J. Vermeulen
  • Amplification methods
    Patent number: 7723038
    Abstract: Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe.
    Type: Grant
    Filed: December 18, 2007
    Date of Patent: May 25, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Walt Mahoney, Nicolaas M. J. Vermeulen, Irina Afonina
  • Single nucleotide polymorphism analysis of highly polymorphic target sequences
    Patent number: 7718374
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Grant
    Filed: January 28, 2008
    Date of Patent: May 18, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
  • Systems and methods for predicting oligonucleotide melting temperature (TS)
    Patent number: 7715989
    Abstract: Software systems and methods for predicting the melting temperature (Tm) and other characteristics of oligonucleotides, including modified oligonucleotides. Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
    Type: Grant
    Filed: June 18, 2002
    Date of Patent: May 11, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Robert O. Dempcy, Alexander A. Gall, Sergey G. Lokhov, Irina A. Afonina, Michael J. Singer, Karen M. Singer, legal representative, Igor V. Kutyavin, Nicolaas M. J. Vermeulen
  • Phosphonate fluorescent dyes and conjugates
    Patent number: 7671218
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Grant
    Filed: August 12, 2005
    Date of Patent: March 2, 2010
    Assignee: Elitech Holdings B.V.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Fluorescent quenching detection reagents and methods
    Patent number: 7662942
    Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
    Type: Grant
    Filed: January 23, 2006
    Date of Patent: February 16, 2010
    Assignee: Elitech Holdings, B.V.
    Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M. J. Vermeulen