Abstract: The present invention relates to the production of ethanol as a product of bacterial fermentation. In particular this invention relates to a novel method of gene inactivation and gene expression based upon homologous recombination. The method is particularly useful in connection with species of Bacillus such as B. stereothermophilus, B. calvodelox, B. caldotenax, B. thermoglucosidasius, B. coagulans, B. licheniformis, B. thermodenitrificans, and B. caldolyticus.
Type:
Grant
Filed:
February 24, 2009
Date of Patent:
January 17, 2012
Assignee:
Elsworth Biotechnology Limited
Inventors:
Muhammed Javed, Fiona Cusdin, Paul Milner, Edward Green
Abstract: Production of ethanol in a thermophilic bacterium can be achieved by transformation of the bacterium with a heterologous gene encoding pyruvate decarboxylase. The bacterium comprises native alcohol dehydrogenase function and does not comprise a gene encoding a functional lactate dehydrogenase. The bacterium may be grown at elevated temperatures to allow the ethanol to be conveniently removed in a vaporized form from the fermentation medium. Traditional mesophilic microorganisms are incapable of growth at the envisioned elevated temperatures.
Type:
Grant
Filed:
March 13, 2007
Date of Patent:
April 6, 2010
Assignee:
Elsworth Biotechnology Limited
Inventors:
Edward Green, Muhammad Javed, Namdar Baghaei-Yazdi
Abstract: The present invention relates to the production of ethanol as a product of bacterial fermentation. In particular this invention relates to a novel method of gene inactivation and gene expression based upon homologous recombination.
Type:
Application
Filed:
April 19, 2005
Publication date:
August 18, 2005
Applicant:
Elsworth Biotechnology Limited
Inventors:
Muhammad Javed, Fiona Cusdin, Paul Milner, Edward Green
Abstract: Sporulation-deficient variants of thermophilic, facultatively anaerobic, Gram-positive bacteria can be generated using a plasmid transformation system based on a novel method of in vivo methylation. Such bacteria exhibit improved ethanol production-related characteristics.
Type:
Grant
Filed:
May 9, 2001
Date of Patent:
December 16, 2003
Assignee:
Elsworth Biotechnology Limited
Inventors:
Edward M. Green, Fiona S. Cusdin, Namdar Baghaei-Yazdi, Muhammad Javed