Abstract: The present application relates to novel and improved methods of achieving virus inactivation during a protein purification process.

Abstract: Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.

Abstract: A normal flow filtration system having a housing with one or more filters contained within a bowl and attached to the outlet of the system. Liquid enters the bowl, contacts the surfaces of the one or more filters. Impurities are trapped in the filter and the filtered liquid flows through to the outlet. Parameter(s) such as flow rate, transmembrane pressure drop and/or time are measured and when met, filtration ceases. A backflushing occurs through the filter(s) so as to remove the impurities from the filter. This backwashed material is directed to an outlet of the bowl that is connected to a drain. Additionally a port formed in the side of the housing adjacent an outer surface of the filter(s) can be used to provide a lateral washing of the filter(s). The system is then recirculated in a downstream manner to reestablish equilibrium and filtration is then restarted.

Abstract: The present invention is a cell cultivating vessel or device, such as a single or multitier flask, including a cover having a top plate, a side wall and a resealable port; an intermediate tray for receiving cells and cell culture media, having a bottom plate, a side wall, and a gap region formed between an interior upwardly angled lip located on an interior portion of the intermediate tray bottom plate and an adjacent outwardly angled side wall portion of the intermediate tray bottom plate, wherein the lip has a outwardly swooping curvilinear edge feature; and a base tray for receiving the cells and cell culture media, including a bottom plate and a side wall.

Abstract: The invention concerns a circuit comprising a bag (11) comprising two flexible films (45, 46) and a press (10) comprising a first (14) and a second (13) shell clamping the bag to form pipes having a passage and edges (41); the first shell comprising a valve (20) which comprises a movable member (24) and a pad (31), which pad has a resting configuration in which a second face (33) of the pad is concave and locally delimits a channel (18) and a pinching configuration in which the second face (33) is convex; said movable member and said pad being configured so that, when said valve is in closed position and said pad in pinching configuration, said passage is pinched by said movable member against said second shell channel and said edges (41) are pinched flat by said movable member against said second shell (13).

Abstract: A method for removing microorganisms from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention. Microorganisms such as bacteria, particularly B. Diminuta, are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a B. Diminuta LRV greater than about 9, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. Another method for removing microorganisms such as bacteria and Mycloplasma, includes passing the liquid through a porous nanofiber containing filtration medium having a microorganism LRV greater than about 8, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. The filtration medium can be in the form of a fibrous electro spun polymeric nanofiber liquid filtration medium mat.

Abstract: The invention relates to a single use cell culture assembly for use in carrying out bioreactions and growing microorganisms, and tissue and cell cultures. The cell culture assembly is designed to receive an insert having a microscope slide upper surface, and includes a well frame having a plurality of partitioned well compartments, adapted to be positioned on the upper surface of the insert, and includes a liquid-impermeable releasable seal positioned on or within the lower edge of the well frame for maintaining a liquid-impermeable barrier between well compartments.

Abstract: The invention concerns a device comprising a base (2) and a door (20), said device having a closed door position in which a circuit (8) of the device comprises a bag comprising two flexible films and connectors of the conveying network, and a press (9) comprising a first she (16) disposed on a front face (5) of said base (2) and a second shed (17) disposed in said door (20); and a hinge system hinging said door (20) relative to said base (2), and disposed only on one side of said door (20) so as to form lateral clearances between said door (20) and said base (2) over the rest of a perimeter of said door (20).

Abstract: The invention concerns a treated water purification system (107) comprising a water flow loop (110), said loop (110) being closed onto a tank (10) of treated water to purify, and said loop (110) successively comprising, in the direction of flow of the water downstream of the tank (10), at least one pump means (102), at least one first filtration means (103), at least one second filtration means (104) and at least one point of use (U), the system (107) being characterized in that it further comprises at least one diversionary pipe (112) linking the first filtration means (103) to the tank (10), and a loop return pipe (114) linking the second filtration means (104) to the tank (10). Method for use of such a system.

Abstract: A disposable container, such as a deformable bag, for a fluid, having one or more inlets and one or more outlets and an impeller assembly within the container to cause mixing, dispersing, homogenizing and/or circulation of one or more ingredients contained or added to the container. The region within the container that can contain liquid is funnel shaped, which allows very low fluid level mixing, dispensing while mixing, and a reduction or elimination of vortex formation.

Abstract: A vacuum filter device comprising a filter body having two holders on opposite sides of a filter. Each holder contains a closed container in a fluid-tight, sealed relationship. The filter is retained by a compression sealing element and the sealing element is maintained in place by a compression element formed of a ring. The compression element is bonded to a portion of the body to hold it, the sealing element and filter in place under a compression seal. Optionally a port in the compression element is formed to align with the vent of the device. Further, an alignment feature on the compression element ensures the port is in alignment with the vent of the device. The device also includes a vacuum port communicating with the downstream side of the filter, and hence the filtrate container.

Abstract: The invention concerns a device comprising: a base (2); a moveable or removable door (20), said device having a closed door position; and in the closed door position, a circuit (8) comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed on said front face (5) of said base (2) and a second shell (17) disposed in said door (20); said bag being clamped between said first shell (16) and said second shell (17) in a state in which conduits of said network for conveying liquid are formed between said films.

Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.

Abstract: A method for transferring a filter membrane (130) bearing a retentate to a reagent pad (310), in which the membrane is mounted in a support frame (120) having a shoulder (121) and a skirt (122) projecting from the shoulder, the pad being mounted on a cassette (300) of complementary form to said skirt, the skirt being adapted to slide sealingly on the cassette until it comes into abutment formed by the shoulder, the method comprising the steps of sealingly engaging the skirt of the membrane support frame on the cassette, establishing a pressure difference between the cassette and the membrane support frame such that the membrane adopts the shape of a dome pointing towards the pad, while maintaining the pressure difference, applying a force on the frame so as to make the skirt slide on the cassette until contact is established between the end of the dome and the pad, maintaining a force on the frame so as to move it downward until it comes into abutment formed by the shoulder while maintaining the pressure diff

Abstract: An illumination system includes a master power supply providing power to several illumination modules. The master power supply is constructed and arranged to generate high-frequency and low-voltage electrical power provided to a primary wire forming a current loop. Each illumination module includes an electromagnetic coupling element and several light sources.

Abstract: Redundant filtration system that includes at least one barrier filter as a process filter, the barrier filter having both hydrophilic and hydrophobic paths, allowing both fluid and gas permeability. The need for vents is eliminated, as is an intermediate drain filter. The device also can be dried in series. The filtration system includes a network of conduits and receptacles, the network receiving liquid raw and/or starting material at one end, conducting it through the process stream defined thereby, and producing the desired liquid product at another end. The network is provided with one or more in-puts for introducing liquid raw material into the fluid process stream, and one or more output ports for discharging fluid out of the fluid process stream. The network is preferably an essentially closed network, and also, preferably sterile and/or aseptic.

Abstract: The invention concerns a circuit comprising a bag (11) comprising two flexible films (45, 46) and a press (10) comprising a first (14) and a second (13) shell clamping the bag to form pipes having a passage and edges (41); the first shell comprising a valve (20) which comprises a movable member (24) and a pad (31), which pad has a resting configuration in which a second face (33) of the pad is concave and locally delimits a channel (18) and a pinching configuration in which the second face (33) is convex; said movable member and said pad being configured so that, when said valve is in closed position and said pad in pinching configuration, said passage is pinched by said movable member against said second shell channel and said edges (41) are pinched flat by said movable member against said second shell (13).

Abstract: The invention concerns a filtration device comprising a manifold providing fluid connection and mechanical connection comprising a clamping plate movably mounted relative to the manifold and adapted to compress at least one cassette against the manifold, means for driving and guiding the clamping plate comprising a rod extending between the manifold and the plate, an actuator provided with a body and a member moveable relative to the body, which member is adapted to move through a predetermined travel between an extended position and a retracted position, the body being carried by the manifold, and the member carrying the rod and driving the plate via the rod to a clamped position in which the member is in an intermediate retracted position in which it has moved through a shorter travel than the predetermined travel relative to its extended position.

Abstract: The purity and titer of virus stocks used for virus clearance studies have a significant influence on study outcome, and impact how well the scale-down model represents the production-scale process. Impurities in virus stocks are particularly important in the testing of small virus retentive filters because these impurities may cause a filter to foul prematurely, leading to underestimation of the true throughput capability of the filter and consequent inappropriate sizing of the production scale unit. In addition, impurities can also affect the levels of virus clearance observed by altering the fouling mechanisms and subsequent fluid passage through the virus filter. Described herein are methods for making, producing and using high purity virus stocks having high titer.