Patents Assigned to Empresa Brasileira de Pesquisa Agropecuaria-Embrapa
  • Patent number: 10894964
    Abstract: The present invention refers to the use of AT(n) insertions in promoter elements for controlling the expression levels of coding sequences in plants. The expression levels of the heat shock protein (Gmhsp17.6-L), when compared in resistant and susceptible individuals in the population, demonstrated that the largest expression levels per quantitative PCR were present in the individuals that contained the largest AT insertions in the promoter region. The invention also refers to gene expression cassettes containing promoter regions of the gene with different numbers of AT insertions fused to the GUS protein, for transforming soybean embryos.
    Type: Grant
    Filed: November 28, 2008
    Date of Patent: January 19, 2021
    Assignee: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA - EMBRAPA
    Inventors: Renata Fuganti, Alexandre Lima Nepomuceno, Francismar Correa Marcelino, Joao Flavio Veloso Silva, Silvana Regina Rochenbach Marin, Cesar Augusto Silveira, Eliseu Binneck
  • Patent number: 10889624
    Abstract: The Present invention relates to molecules isolated from the nucleic acid that encodes spider web proteins or fragments of these or other derivatives of these. The invention also refers to a chimerical gene and an expression vector containing molecules isolated from the nucleic acid that codes for proteins related to the webs of Nephilengys, cruentata, Avicularia juruensis and Parawixia bistriata spiders. Another embodiment of the present invention are transformed cells containing a chimerical gene or an expression vector of the present invention. Yet another embodiment of the present invention relates to a method for obtaining genetically modified organisms containing inventive chimerical genes or expression vectors and a method for obtaining recombinant proteins from the silks of Nephilengys, cruentata, Avicularia juruensis and Parawixia bistriata spiders. Finally, the invention describes products, such as biofilaments and compositions, using the recombinant proteins of the present invention.
    Type: Grant
    Filed: January 24, 2013
    Date of Patent: January 12, 2021
    Assignees: Empresa Brasileira de Pesquisa Agropecuaria-Embrapa, Fundaçao Universidade de Brasilia
    Inventors: Elibio Leopoldo Rech Filho, Natalia Cristina Verza Ferreira, Giovanni Rodrigues Vianna, Felipe Rodrigues da Silva, Francisco José Lima Aragão, Luiz Alberto Colnago, Alan Carvalho Andrade, Daniela Matias de Carvalho Bittencourt, Pedro Ismael da Silva Junior, Betúlia de Morais Souto, Luisa de Moraes Madeira, Paulo César Motta
  • Patent number: 10687529
    Abstract: The present application relates generically to compositions used for controlling agricultural pests and, in a particular, to compositions usable for combating nematodes. The application presents a feasible alternative to the use of nematotoxic compounds that are harmful to the environment and that may damage human health. This alternative includes a nematotoxic composition for controlling nematodes that may be used on plants, parts of plants and on the soil. The respective technology results from unexpected results achieved from experiments carried out with extracts of the plant Canavalia ensiformis, resulting in an unheard-of nematotoxic composition comprising a specific combination of substances that are naturally found in the extract and that act synergistically.
    Type: Grant
    Filed: December 26, 2013
    Date of Patent: June 23, 2020
    Assignees: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA-EMBRAPA, THE UNIVERSITY OF MELBOURNE
    Inventors: Thales Lima Rocha, Raphael Garcia de Sousa Evaristo, Maria Fátima Grossi de Sá, Djair Dos Santos de Lima e Souza, Maria Cristina Mattar da Silva, Vera Lúcia Perussi Polez, Ute Roessner, Tony Bacic
  • Publication number: 20200187438
    Abstract: The present invention presents a method for obtaining female inbred lines from Asteracea hybrids, using the species Helianthus annuus as a model. The method of the invention is based on the modification of lines with the fertility restorer gene (Rf), obtained from self-pollination of hybrids, in lines presenting normal cytoplasm and not containing the Rf gene. Further, derived male sterile lines were developed. Through the use of this methodology it was possible to obtain female lines from commercial hybrids of sunflower.
    Type: Application
    Filed: February 27, 2020
    Publication date: June 18, 2020
    Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA - EMBRAPA
    Inventors: Claudio Guilherme Portela de CARVALHO, José Francisco Ferraz de TOLEDO
  • Patent number: 10098840
    Abstract: The present invention relates to the development of two types of polymer nanocapsules to encapsulate cloxacillin benzathine, an antimicrobial drug. More specifically, this invention relates to a new form of treatment for mastitis in dairy cattle. Two nanocapsule formulations have been developed and can be used to encapsulate various drugs, besides cloxacillin benzathine, providing a new therapy for mastitis in cows, avoiding the inconvenience of the use of high doses of drugs used in conventional formulations, thus contributing to an improvement in milk quality.
    Type: Grant
    Filed: June 1, 2011
    Date of Patent: October 16, 2018
    Assignees: UNIVERSIDADE FEDERAL DE OURO PRETO, EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA-EMBRAPA, FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAIS-FAPEMIG
    Inventors: Vanessa Carla Furtado Mosqueira, Raquel Silva Araújo, Humberto de Mello Brandão
  • Patent number: 9961848
    Abstract: Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location comprises genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO:5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.
    Type: Grant
    Filed: April 6, 2015
    Date of Patent: May 8, 2018
    Assignees: Empresa Brasileira De Pesquisa Agropecuaria-Embrapa, BASF Agrochemical Products B.V.
    Inventors: Dale Carlson, Francisco Jose Lima Aragao, Carlos Alberto Arrabal Arias, Luiz Louzano, Bruce M. Luzzi, Tim Malefyt, Elibio Leopoldo Rech Filho, Siyuan Tan, Adolfo Ulbrich, Tadashi Yotsumoto, Ute Linemann
  • Patent number: 9756796
    Abstract: A pressure measuring and flow-rate controlling device includes: a flattening plate including a fluid cavity in the flattening plate; a flexible contact membrane provided on said flattening plate; a support member configured to secure the flattening plate against a sample; a pressure adjuster; an inlet tube connected to the pressure adjuster at a first end of the inlet tube, the inlet tube connected to a first end of the fluid cavity at a second end opposite to the first end of the inlet tube; and an outlet tube connected to a second end opposite of the first end of the fluid cavity, where the pressure adjuster is configured to control a flow rate of a fluid passing through the fluid cavity based on cell pressure of the sample.
    Type: Grant
    Filed: December 27, 2012
    Date of Patent: September 12, 2017
    Assignee: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA-EMBRAPA
    Inventor: Adonai Gimenez Calbo
  • Patent number: 9750247
    Abstract: The present invention relates generically to compositions used for controlling agricultural pests and, in a particular embodiment, to compositions usable for combating nematodes. The invention presents a feasible alternative to the use of nematotoxic compounds that are harmful to the environment and that may damage human health. This alternative is embodied through a nematotoxic composition for controlling nematodes and that may be used on plants, parts of plants and on the soil. The respective technology results from unexpected results achieved from experiments carried out with extracts of the plant Canavalia ensiformis, resulting in an unheard-of nematotoxic composition comprising a specific combination of substances that are naturally found in the extract and that act synergistically.
    Type: Grant
    Filed: December 26, 2013
    Date of Patent: September 5, 2017
    Assignees: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA-EMBRAPA, UNITVERSITY OF MELBOURNE
    Inventors: Thales Lima Rocha, Raphael Garcia de Sousa Evaristo, Maria Fátima Grossi de Sá, Djair dos Santos de Lima e Souza, Maria Cristina Mattar da Silva, Vera Lúcia Perussi Polez, Ute Roessner, Tony Bacic
  • Publication number: 20160281101
    Abstract: The present invention relates to a polynucleotide sequence capable of efficiently modifying the expression of one or more genes of interest in leaves, in particular in plants of the Glycine genus, and the tools to obtain genetically-modified plants using this sequence and the use thereof. The usage possibilities of the invention are broad, prominently, the creation of new plant varieties resistant to diseases and leaf-attacking pests, expression of transgenes that increase the photosynthetic efficiency of the plant, guiding the expression of proteins of interest as antibodies and drugs that may easily be isolated from leaves.
    Type: Application
    Filed: March 18, 2014
    Publication date: September 29, 2016
    Applicants: Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA, Fundação Universidade de Brasília - FUB - UNB
    Inventors: Juliana Dantas de Almeida, Leila Maria Gomes Barros, Renata Henrique Santana, Ricardo Vilela Abdelnoor, Felipe Rodrigues da Silva
  • Publication number: 20160272982
    Abstract: The present invention relates to a new promoter of the expression of genes in plants. More specifically, the invention relates to regulatory sequences of polynucleotides that are isolated from soy plants and can initiate and activate polynucleotide transcription, and to the use of these regulatory sequences to modify the transcription of endogenous and/or heterologous polynucleotides and to produce polypeptides. The invention further describes DNA constructs that contain the promoter of the gene of the supposed flavonol-sulfotransferase protein in soy plants, which promoter is functionally linked to a heterologous and/or endogenous gene. Moreover, the invention relates to the use of these constructs in the form of expression cassettes, expression vectors, recombinant vectors and in plants, plant cells or transgenic protoplasts.
    Type: Application
    Filed: March 18, 2014
    Publication date: September 22, 2016
    Applicants: Fundação Universidade de Brasília, Empresa Brasileira de Pesquisa Agropecuária - Embrapa
    Inventors: Juliana Dantas de Almeida, Leila Maria Gomes Barros, Renata Henrique Santana, Ricardo Vilela Abdelnoor, Felipe Rodrigues da Silva
  • Publication number: 20160223450
    Abstract: The present invention provides a water tension sensor with a non-sintered core that allows batches of similar and standard sensors to be manufactured in a simple manner, for operation with air and with an air flow outlet, which sensor can be used to read water tension and trigger dripping. The sensor according to the invention is ideal for manually reading soil water tension and for automating irrigation systems based on a static pressure or gas propulsion mechanism.
    Type: Application
    Filed: April 24, 2014
    Publication date: August 4, 2016
    Applicants: Empresa Brasileira de Pesquisa Agropecuaria - Embrapa, Tecnicer Techologia Ceramica LTDA
    Inventors: Adonai Gimenez Calbo, Carlos Manoel Pedro Vaz, Waldir Aparecido Marouelli, Luis Fernando Porto
  • Publication number: 20160000086
    Abstract: The present invention relates to the control of infestation of pests by inhibiting or reducing the expression of genes of the family of chitin synthase. The invention further provides methods and compositions for controlling pests by feeding them with one or more double-stranded RNA molecules provides by the present invention. The invention further describes a method of obtaining transgenic plants that express double-stranded RNA molecules. The present invention is preferably used for cotton-plants.
    Type: Application
    Filed: December 27, 2013
    Publication date: January 7, 2016
    Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA- EMBRAPA
    Inventors: Maria Fátima Grossi de Sá, Maria Cristina Mattar Da Silva, Leonardo Lima Pepino de Macedo, Alexandre Augusto Pereira Firmino, Roberta Ramos Coelho, Isabela Tristan Lourenço
  • Publication number: 20150336854
    Abstract: The present invention falls into the context of green chemistry and generically relates to a fertilizing and phytoprotective formulation and, in particular embodiment, to a film forming formulation that induces resistance to plants. The respective formulation, when applied to plants and/or fruits, results in the formation of a film on the surface of the material, which has a characteristic of photoprotection against UV-B and UV-C radiations, resistance kept in water, even after high hygroscopicity, greater stability at high ambient temperatures, formation of desired porosity and surface homogeneity.
    Type: Application
    Filed: December 26, 2013
    Publication date: November 26, 2015
    Applicants: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA- EMBRAPA, Universidae Federal do Rio Grande do Sul
    Inventors: Angela DINIZ CAMPOS, César BAUER GOMES, Fabiane GRECCO DA SILVA PORTO, Irene Teresinha SANTOS GARCIA, Irajá FERREIRA ANTUNES, Bernardo UENO, Luis Antônio SUITA DE CASTRO, José Francisco MARTINS FERREIRA, Walkyria BUENO SCIVITTARO
  • Publication number: 20150313216
    Abstract: The present invention relates generically to compositions used for controlling agricultural pests and, in a particular embodiment, to compositions usable for combating nematodes. The invention presents a feasible alternative to the use of nematotoxic compounds that are harmful to the environment and that may damage human health. This alternative is embodied through a nematotoxic composition for controlling nematodes and that may be used on plants, parts of plants and on the soil. The respective technology results from unexpected results achieved from experiments carried out with extracts of the plant Canavalia ensiformis, resulting in an unheard-of nematotoxic composition comprising a specific combination of substances that are naturally found in the extract and that act synergistically.
    Type: Application
    Filed: December 26, 2013
    Publication date: November 5, 2015
    Applicants: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA- EMBRAPA, THE UNIVERSITY OF MELBOURNE
    Inventors: Thales Lima Rocha, Raphael Garcia de Sousa Evaristo, Maria Fátima Grossi de Sá, Djair Dos Santos de Lima e Souza, Maria Cristina Mattar da Silva, Vera Lúcia Perussi Polez, Ute Roessner, Tony Bacic
  • Publication number: 20150197762
    Abstract: The present invention relates to a promoter sequence specific for expression of genes of interest in fruit endosperm. The invention further describes DNA constructs that contain the promoter, as well as the method using such constructs to produce transgenic plants, plant cells or protoplasts. The expression of the transgene only in the organ of interest enables accumulation of the exogenous transcript only in the fruit and constitutes an advantage over the constitutive expression, since it favors implementation of strategies that aim at obtaining cultivars having higher aggregate value like: adaptation to environmental stress, tolerance to pathogens, pests and agricultural defensives, increase in the nutritional and therapeutic value and a new alternative to systems of expression in plant organism, generating more competitive cultivars at lower costs.
    Type: Application
    Filed: April 9, 2013
    Publication date: July 16, 2015
    Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA - EMBRAPA
    Inventors: Juliana Dantas Dealmeida, Leila Maria Gomes Barros, Mauro Carneiro, Alan Carvalho Andrade, Felipe Rodrigues Da Silva, Luiz Filipe Protasio Pereira, Michelle Guitton Cotta, Mirian Therezinha Souza Da Eira
  • Publication number: 20150059866
    Abstract: The invention relates to a system for measuring cell turgor pressure (dendroturgometer), which can also operate as a firmometer. The system can also be used as an automatic dripper, wherein in this embodiment it is kept pressed by means of a flattening plate (1) against the organ (4), as well as by employing a brace (6) provided with a spring (5) that applies stable force of a magnitude higher than the product between the water pressure, regulated by a pressure adjuster (10) and the fluid layer (2) under the membrane (3), on which the plant exerts compression and obstructs the water flow. The dripping is started when the transpiration provides a drop in the cell turgor pressure of the plant, so that the resulting pressure will be lower than the adjusted water pressure. This system may also be miniaturized and used for measuring the vegetable turgor pressure by using water pressurization with an plunger (11).
    Type: Application
    Filed: December 27, 2012
    Publication date: March 5, 2015
    Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA - EMBRAPA
    Inventor: Adonai Gimenez Calbo
  • Publication number: 20140366217
    Abstract: The present invention relates to the field of insect pests control, using methods and compositions which comprise of alpha-amylase analogous mutant inhibitors (?AIs). More specifically, the invention provides new ?AIs analogous mutant molecules for controlling insect pests, in particular boll weevils (Anthonomus grandis), partially or totally presenting reduction of the amylolytic activity of the digestive enzymes in the intestinal lumen of the insect. Other aspects of the invention include gene constructs containing the nucleic acid molecules that code for the alpha-amylase inhibitors, heterologous expression methods of the new molecules in the active form, and the use of these molecules for controlling insect pests. The invention also provides analogous synthetic genes optimized for their transformation and expression in plants.
    Type: Application
    Filed: June 8, 2012
    Publication date: December 11, 2014
    Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA - EMBRAPA
    Inventors: Maria Fatima Grossi De Sa, Maria Cristina Mattar Da Silva, Rafael Perseguini Del Sarto, Thales Lima Rocha
  • Publication number: 20140206851
    Abstract: The Present invention relates to molecules isolated from the nucleic acid that encodes spider web proteins or fragments of these or other derivatives of these. The invention also refers to a chimerical gene and an expression vector containing molecules isolated from the nucleic acid that codes for proteins related to the webs of Nephilengys, Cruentata, Avicularia Juruensis and Parawixia Bistriata spiders. Another embodiment of the present invention are transformed cells containing a chimerical gene or an expression vector of the present invention. Yet another embodiment of the present invention relates to a method for obtaining genetically modified organisms containing inventive chimerical genes or expression vectors and a method for obtaining recombinant proteins from the silks of Nephilengys, Cruentata, Avicularia Juruensis and Parawixia Bistriata spiders. Finally, the invention describes products, such as biofilaments and compositions, using the recombinant proteins of the present invention.
    Type: Application
    Filed: January 24, 2013
    Publication date: July 24, 2014
    Applicants: Fundacao Universidade de Brasilia, Empresa Brasileira de Pesquisa Agropecuaria - EMBRAPA
    Inventors: Empresa Brasileira de Pesquisa Agropecuaria - Embrapa, Fundacao Universidade de Brasilia
  • Publication number: 20140086875
    Abstract: The objective of the current invention is the control of agricultural infestations based on alternative methods that are less aggressive to the environment and that are harmless to other invertebrates and to man; for instance biological control using bacterial strains belonging to species of the genera Bacillus, Brevibacillus and/or Paenibacillus. A first embodiment of the invention refers to compositions based on the systemic utilization of bacterial strains belonging to species of the genera Bacillus, Brevibacillus and/or Paenibacillus to protect plants by using the composition containing the above referred bacteria in wild-type, conjugated, mutant or transgenic forms and/or the substances produced by them. A second embodiment of the invention refers to a method of bioinsecticide application based on bacteria from the genera Bacillus, Brevibacillus and/or Paenibacillus.
    Type: Application
    Filed: November 27, 2013
    Publication date: March 27, 2014
    Applicants: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA - EMBRAPA, UNIVERSITY COLLEGE CARDIFF CONSULTANTS LIMITED, BTHEK BIOTECNOLOGIA LTDA.
    Inventors: Rose Gomes Monnerat Solon DE PONTES, Carlos Marcelo Silveira SOARES, Colin BERRY
  • Publication number: 20140075594
    Abstract: The present invention refers to the use of AT(n) insertions in promoter elements for controlling the expression levels of coding sequences in plants. The expression levels of the heat shock protein (Gmhsp17.6-L), when compared in resistant and susceptible individuals in the population, demonstrated that the largest expression levels per quantitative PCR were present in the individuals that contained the largest AT insertions in the promoter region. The invention also refers to gene expression cassettes containing promoter regions of the gene with different numbers of AT insertions fused to the GUS protein, for transforming soybean embryos.
    Type: Application
    Filed: November 28, 2008
    Publication date: March 13, 2014
    Applicant: Empresa Brasileira de Pesquisa Agropecuaria- EMBRAPA
    Inventors: Renata Fuganti, Alexandre Lima Nepomuceno, Francismar Correa Marcelino, Joao Flavio Veloso Silva, Silvana Regina Rochenbach Marin, Cesar Augusto Silveira, Eliseu Binneck