Abstract: The present invention relates to a test tube preparation device which is compact, light and more mobile and improve the spatial layout of an installing unit, a transfer unit and a label attaching unit and the constitution of the units. According to an aspect, the invention may comprise a frame in which right and left installing units where a plurality of test tubes are vertically installed are provided on the upper part and at least one guide hole which allows the test tube to move by passing through the hole; a gripper transfer unit transfers the test tubes installed in the right and left installing units to the guide hole; a gripper pinches or separates the test tubes; and a labelling unit attaches a label to the waist of the test tube transferred through the guide hole.