Abstract: The invention pertains to a single polypeptide chain binding molecule which has binding specificity and affinity substantially similar to the binding specificity and affinity of the light and heavy chain aggregate variable region of an antibody, to genetic sequences coding therefor, and to recombinant DNA methods of producing such molecule and uses for such molecule.
Type:
Grant
Filed:
June 6, 1995
Date of Patent:
July 9, 1996
Assignee:
Enzon Labs, Inc.
Inventors:
Robert C. Ladner, Robert E. Bird, Karl Hardman
Abstract: The invention pertains to a single polypeptide chain binding molecule which has binding specificity and affinity substantially similar to the binding specificity and affinity of the light and heavy chain aggregate variable region of an antibody, to genetic sequences coding therefor, and to recombinant DNA methods of producing such molecule and uses for such molecule.
Type:
Grant
Filed:
June 6, 1995
Date of Patent:
May 21, 1996
Assignee:
Enzon Labs Inc.
Inventors:
Robert C. Ladner, Robert E. Bird, Karl Hardman
Abstract: The invention pertains to a single polypeptide chain binding molecule which has binding specificity and affinity substantially similar to the binding specificity and affinity of the light and heavy chain aggregate variable region of an antibody, to genetic sequences coding therefor, and to recombinant DNA methods of producing such molecule and uses for such molecule.
Type:
Grant
Filed:
April 1, 1993
Date of Patent:
October 3, 1995
Assignee:
Enzon Labs, Inc.
Inventors:
Robert C. Ladner, Robert E. Bird, Karl Hardman
Abstract: The present invention provides a method for redesigning proteins to increase the stability of the protein by altering amino acid residue(s) that are in close proximity to the protein's metal ion binding site(s). Further, the invention is directed to proteins that have been redesigned to have enhanced stability according to the methods of this invention.
Type:
Grant
Filed:
October 19, 1990
Date of Patent:
November 9, 1993
Assignee:
Enzon Labs Inc.
Inventors:
Michael W. Pantoliano, Barry C. Finzel, Philip N. Bryan
Abstract: Vectors are provided that are capable of expressing, in microbial transformants, a protein having the native amino acid sequence of a bioadhesive precursor protein of a marine animal selected from the group consisting of mussels, barnacles, and oysters. The bioadhesive precursor protein can be expressed in transformants, recovered and converted to a bioadhesive protein by hydroxylation.
Type:
Grant
Filed:
May 25, 1990
Date of Patent:
April 13, 1993
Assignee:
Enzon Labs Inc.
Inventors:
Kathy J. Maugh, David M. Anderson, Robert Strausberg, Susan L. Strausberg
Abstract: Vectors are provided that are capable of expressing, in microbial transformants, a protein having the native amino acid sequence of a bioadhesive precursor protein of a marine animal selected from the group consisting of mussels, barnacles, and oysters. The bioadhesive precursor protein can be expressed in transformants, recovered and converted to a bioadhesive protein by hydroxylation.
Type:
Grant
Filed:
August 16, 1991
Date of Patent:
April 13, 1993
Assignee:
Enzon Labs, Inc.
Inventors:
Kathy J. Maugh, David M. Anderson, Robert Strausberg, Susan L. Strausberg, Russ McCandliss, Tena Wei, David Filpula
Abstract: This invention is directed to a novel hybrid regulatory region for directing and regulating transcription and translation of a gene sequence positioned downstream from the hybrid regulatory region. This hybrid regulatory region includes the promoter sequence of the phage lambda P.sub.R promoter-operator region fused to the operator sequence of the phage lambda P.sub.L promoter-operator region.
Type:
Grant
Filed:
July 2, 1990
Date of Patent:
November 10, 1992
Assignee:
Enzon Labs Inc.
Inventors:
Dorothea H. Scandella, Keith H. McKenney
Abstract: Recombinant production of a bioadhesive precursor protein analog comprising three, five, ten, fifteen or twenty repeated decapeptides of the formula Ala-Lys-Pro-Ser-Tyr-Pro-Pro-Thr-Tyr-Lys is disclosed.