Patents Assigned to EpiCypher, Inc.
  • Quantification of nucleosome modifications using chemically-defined recombinant nucleosomes
    Patent number: 12104199
    Abstract: The invention relates to the use of recombinant/semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively “modified nucleosomes” or “nucleosome modifications”) from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.
    Type: Grant
    Filed: July 24, 2020
    Date of Patent: October 1, 2024
    Assignee: EpiCypher, Inc.
    Inventors: Martis William Cowles, Matthew F. Whelihan, Andrea L. Johnstone, Michael-Christopher Koegh, Zu-Wen Sun, Nathan W. Hall, Matthew R. Marunde
  • Assays for nucleosome remodeling activity
    Patent number: 11493520
    Abstract: The invention relates to assays for nucleosome remodeling activity using functionalized recombinant mononucleosomes. The functionalized recombinant mononucleosomes comprise a histone octamer comprising recombinant histone H2A, H2B, H3 and H4 proteins and a DNA template comprising a nucleosome positioning sequence that effectively positions the histone octamer and a signal site. The invention further relates to methods of using the assay to quantify enzymatic activity of remodeling enzymes and identifying modulators of remodeling enzyme activity.
    Type: Grant
    Filed: May 18, 2018
    Date of Patent: November 8, 2022
    Assignee: EpiCypher, Inc.
    Inventors: Michael-Christopher Keogh, Martis William Cowles, Matthew Fruge Whelihan
  • Quantification of nucleosome modifications using chemically-defined recombinant nucleosomes
    Patent number: 10787697
    Abstract: The invention relates to the use of recombinant/semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively “modified nucleosomes” or “nucleosome modifications”) from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.
    Type: Grant
    Filed: November 8, 2019
    Date of Patent: September 29, 2020
    Assignee: EpiCypher, Inc.
    Inventors: Martis William Cowles, Matthew F. Whelihan, Andrea L. Johnstone, Michael-Christopher Koegh, Zu-Wen Sun, Nathan W. Hall, Matthew R. Marunde