Abstract: The present invention relates to a method and apparatus for monitoring a real-time quantification of multiple target molecules during their binding on capture molecules of a micro-array. The method comprises the steps of: placing, in a chamber (14), a support (15) having fixed upon its surface a micro-array comprising at least 5 capture molecules (20) being immobilized in specifically localized areas (21) of said support; introducing said labeled target molecules solution (13) into the chamber; incubating said labeled target molecules under stable and controlled temperature conditions to allow the binding between said target and capture molecules; directing an excitation light (2) from a light source (1) on the surface of the micro-array; measuring the electromagnetic light emission (7) from the bound target molecules in response to said excitation light in presence of the solution containing the target molecules wherein the surface of emission for a localized area is comprised between about 0.

Abstract: The present invention relates to a method and apparatus for monitoring on a micro-array a PCR amplification of a nucleotide molecule being present in a solution.

Abstract: A method for obtaining an activation profile of a biological sample by disposing onto a solid support in a pre-determined spatial arrangement a subset of capture molecules able to interact with one or more activated transcription factor(s) present in the biological sample, contacting the biological sample upon the solid support under conditions allowing their interaction, monitoring signals resulting from their interaction, and providing a cellular activation profile from the detected signals.

Abstract: An amplification and micro-array detection method is performed in a same buffer and/or in a same reaction chamber. A kit for an amplification of multiple nucleotide targets, uses low primer concentration.

Abstract: The present invention is related to a method and kit (or device) for the detection and/or the quantification of biomarkers related to tumorigenesis. Said method is advantageously used to propose or adapt an anti-tumoral therapeutic protocol to be administered to a subject. Furthermore, said method and kit (or device) are a technical platform for the identification of new compounds, which are preferably used at (a) specific step(s) of an anti-tumoral therapeutic protocol.

Abstract: The present invention is related to a method for detecting and/or quantifying hierarchical (regulated) molecular changes of a cell in response to any external stimulus.

Abstract: A method for identifying a genetically modified plant by identification and/or quantification of different, multiple nucleotide sequence elements corresponding to at least a portion of an exogenous nucleotide sequence integrated into the genome of the genetically modified plant. The method involves amplifying a nucleic acid sequence element into target nucleic acid sequences, contacting the target nucleic acid sequences with single stranded capture nucleotide sequences, detecting binding of the target nucleotide sequences, constructing a genetic map, and identifying the genetically modified plant.

Abstract: A method for obtaining an activation profile of a biological sample by disposing onto a solid support in a pre-determined spatial arrangement a subset of capture molecules able to interact with one or more activated transcription factor(s) present in the biological sample, contacting the biological sample upon the solid support under conditions allowing their interaction, monitoring signals resulting from their interaction, and providing a cellular activation profile from the detected signals.

Abstract: The present invention is related to a method, kit and computer program for detecting the presence in a sample of an unspecified Genetically Modified Organism (GMO).

Abstract: A method quantifies a target compound selected from the group consisting of a polynucleotide or a protein present in a sample solution. The method includes putting into contact a target compound with a capture probe and detecting signals resulting from the binding between the target compound and its corresponding capture probe and resulting from the printed detection molecule in the different discrete regions. The method obtains a detection curve of the detected signals of the detection molecule and converts the signal obtained from the target compound bound to a specific capture probe into a concentration value and quantifies the target compound by converting the concentration value into a target amount using a target concentration curve.

Abstract: The present invention relates to a method for obtaining an activation profile of a biological sample comprising disposing onto a solid support in a pre-determined spatial arrangement a subset of capture molecules able to interact with one or more activated transcription factor(s) present in the biology cell sample, contacting the biological sample upon the solid support under conditions allowing their interaction, monitoring signals resulting from their interaction, and providing a cellular activation profile from the detected signals.

Abstract: The present invention is related to a screening, detection and/or quantification method of one or more transcriptional factor(s) (1) possibly present in a biological sample, said method comprising the steps of: possibly extracting and isolating said transcriptional factor (1) from said biological sample, putting into contact the transcriptional factor (1) with a double-stranded DNA sequence (2) bound to an insoluble solid support (3), and detecting and/or quantifying said fixed transcriptional factor (1), said double-stranded DNA sequence having a specific sequence able to be fixed by the transcriptional factor (1) and being preferably located at a distance of at least about 6.8 nm from the surface of the solid support (3), and said double-stranded DNA sequence being bound to the surface of the insoluble solid support (3) at a concentration of at least 0.01 pmole/cm2 of solid support surface (3). The present invention is also related to the kit comprising means and media for performing said method.

Abstract: The present invention is related to a method and kit (or device) for identifying and/or quantifying in an array mRNA nucleotide sequences related to resistance to antibiotic(s) being expressed in a (micro)organism present in a sample.

Abstract: The invention relates to a method and a kit for the specific identification and/or quantification of one or several among at least 7 organisms or parts thereof, in a biological sample being possibly contaminated by at least 4 other organisms, by detecting at least one nucleotide sequence specific of each of the organisms possibly present in said biological sample, wherein said nucleotide sequence is homologous with at least 4 other nucleotide sequences.

Abstract: The present invention concerns a new and simplified method for obtaining a polymer surface activated with a high density of aldehyde functions which are stable with time and ready to react covalently with amino groups present on a great number of different molecules used as capture probes according to a microarray.