Patents Assigned to EPSILON MOLECULAR ENGINEERING INC.
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Publication number: 20240117012Abstract: Provided is an antibody that binds to the N-protein of SARS-CoV-2, and a method for utilizing the antibody.Type: ApplicationFiled: February 21, 2022Publication date: April 11, 2024Applicants: Kao Corporation, Epsilon Molecular Engineering, Inc.Inventors: Shunsuke INAURA, Yuta MATSUMURA, Takuto TOJO, Atsushi NAGAMI
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Publication number: 20230365659Abstract: A peptide which binds to SARS-CoV-2 and its usage are provided. The peptide which binds to SARS-CoV-2 comprises one or more structural domain comprising CDR3 consisting of an amino acid sequence of any of SEQ ID NOs: 1 to 9 or an amino acid sequence obtained by substituting at least one amino acid in the amino acid sequence with another amino acid.Type: ApplicationFiled: October 1, 2021Publication date: November 16, 2023Applicants: EPSILON MOLECULAR ENGINEERING, INC., THE KITASATO INSTITUTE, Kao CorporationInventors: Hidekazu Masaki, Shigefumi KUMACHI, Ryo YONEHARA, Yuta MATSUMURA, Hibiki KAWANO, Takuto TOJO, Takuya MORIMOTO, Kazunhiko KATAYAMA, Kei HAGA, Reiko TODAKA, Akihito SAWADA, Tomomi TAKANO
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Patent number: 11634705Abstract: The purpose of the present invention is to provide a method for identifying antibody CDR3 clusters using high speed in vitro screening a library selected from the group consisting of cDNA library and nucleic acid aptamer library comprising: (i) preparing a positive spherical shaped structure by binding a target molecule to a spherical shaped molecule, wherein the target molecule is immobilized on the positive spherical shaped structure, wherein the positive spherical shaped structure may contain a fluorescent label; (ii) preparing a negative spherical shaped structure, wherein the target molecule is not immobilized on the negative spherical shaped structure, wherein the negative spherical shaped structure may contain a fluorescent label; (iii) forming a positive spherical shaped conjugate or a negative spherical shaped conjugate by binding a target detecting molecule capable of binding to the target molecule to the positive spherical shaped structure or to the negative spherical structure, wherein the targetType: GrantFiled: September 18, 2020Date of Patent: April 25, 2023Assignee: EPSILON MOLECULAR ENGINEERING INC.Inventors: Naoto Nemoto, Toshiki Miyajima, Yuta Matsukawa
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Publication number: 20210381026Abstract: The present invention provides an improved immuno-PCR method by using cDNA display comprising the steps of: immobilizing a first antibody having a binding site to a solid phase: contacting a sample fluid to said antibody to bind a target molecule in said sample fluid; contacting said target molecule to a cDNA display being composed of a backbone being composed of a double strand and a side chain having a second antigen binding site to which the second antibody is bound; and conducting polymerase chain reaction to detect said cDNA quantitatively. According to the improved immune-PCR method of the present invention, the target molecule is screened and obtained quantitatively, because it uses cDNA display being composed of one protein/peptide and one DNA.Type: ApplicationFiled: August 21, 2021Publication date: December 9, 2021Applicants: EPSILON MOLECULAR ENGINEERING INC., Saitama UniversityInventors: Naoto Nemoto, Hironori Anzai, Takeru Suzuki, Shigefumi Kumachi, Takuya Terai
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Publication number: 20210079381Abstract: The purpose of the present invention is to provide a method for identifying antibody CDR3 clusters using high speed in vitro screening a library selected from the group consisting of cDNA library and nucleic acid aptamer library comprising: (i) preparing a positive spherical shaped structure by binding a target molecule to a spherical shaped molecule, wherein the target molecule is immobilized on the positive spherical shaped structure, wherein the positive spherical shaped structure may contain a fluorescent label; (ii) preparing a negative spherical shaped structure, wherein the target molecule is not immobilized on the negative spherical shaped structure, wherein the negative spherical shaped structure may contain a fluorescent label; (iii) forming a positive spherical shaped conjugate or a negative spherical shaped conjugate by binding a target detecting molecule capable of binding to the target molecule to the positive spherical shaped structure or to the negative spherical structure, wherein the targetType: ApplicationFiled: September 18, 2020Publication date: March 18, 2021Applicant: EPSILON MOLECULAR ENGINEERING INC.Inventors: Naoto NEMOTO, Toshiki MIYAJIMA, Yuta MATSUKAWA
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Patent number: 10781442Abstract: The purpose of the present invention is to provide a high-speed in vitro screening method for any library selected from the group consisting of a cDNA display library and a nucleic acid aptamer library. This high-speed in vitro screening method involves: (i) a step for preparing positive spherical structures formed by immobilizing a target molecule on a spherical structure and negative spherical structures having no target molecules immobilized thereon; (ii) a step in which a target detection molecule, selected from the aforementioned library having a library size of greater than or equal to 1010, is bonded on each spherical structure to obtain spherical conjugates; (iii) a step in which the spherical conjugates are sorted into positive spherical conjugates and negative spherical conjugates with a cell sorter; (v) a step for supplying the nucleic acid on the surface of the sorted spherical conjugates for PCR; (vi) and a repetition step for repeating steps (i) to (v) above using DNA obtained by PCR.Type: GrantFiled: September 28, 2018Date of Patent: September 22, 2020Assignee: EPSILON MOLECULAR ENGINEERING INC.Inventors: Naoto Nemoto, Toshiki Miyajima, Yuta Matsukawa
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Publication number: 20190085322Abstract: The purpose of the present invention is to provide a high-speed in vitro screening method for any library selected from the group consisting of a cDNA display library and a nucleic acid aptamer library. This high-speed in vitro screening method involves: (i) a step for preparing positive spherical structures formed by immobilizing a target molecule on a spherical structure and negative spherical structures having no target molecules immobilized thereon; (ii) a step in which a target detection molecule, selected from the aforementioned library having a library size of greater than or equal to 1010, is bonded on each spherical structure to obtain spherical conjugates; (iii) a step in which the spherical conjugates are sorted into positive spherical conjugates and negative spherical conjugates with a cell sorter; (v) a step for supplying the nucleic acid on the surface of the sorted spherical conjugates for PCR; (vi) and a repetition step for repeating steps (ii) to (v) above using DNA obtained by PCR.Type: ApplicationFiled: September 28, 2018Publication date: March 21, 2019Applicant: EPSILON MOLECULAR ENGINEERING INC.Inventors: Naoto NEMOTO, Toshiki MIYAJIMA, Yuta MATSUKAWA