Patents Assigned to Esoterix Genetic Laboratories LLC
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Patent number: 10988801Abstract: Disclosed are methods and systems for enumeration of nucleic acids, including for the detection of rare events in a biological sample. In certain embodiments, the method may comprise arranging polynucleotides obtained from a biological sample to form a plurality of reaction sites, wherein each reaction site contains on average one polynucleotide; amplifying the polynucleotides in the plurality of reaction sites; determining by nucleic acid hybridization (i) a first number of first reaction sites containing a target nucleic acid sequence, or a portion thereof, and (ii) a second number of second reaction sites containing a reference nucleic acid sequence, or a portion thereof; comparing the first number of the first reaction sites to the second number of the second reaction sites to determine the relative amount of the target nucleic acid in the biological sample.Type: GrantFiled: December 5, 2018Date of Patent: April 27, 2021Assignee: Esoterix Genetic Laboratories, LLCInventors: Thomas Scholl, Brant Hendrickson
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Publication number: 20190292585Abstract: Disclosed are methods and systems for enumeration of nucleic acids, including for the detection of rare events in a biological sample. In certain embodiments, the method may comprise arranging polynucleotides obtained from a biological sample to form a plurality of reaction sites, wherein each reaction site contains on average one polynucleotide; amplifying the polynucleotides in the plurality of reaction sites; determining by nucleic acid hybridization (i) a first number of first reaction sites containing a target nucleic acid sequence, or a portion thereof, and (ii) a second number of second reaction sites containing a reference nucleic acid sequence, or a portion thereof; comparing the first number of the first reaction sites to the second number of the second reaction sites to determine the relative amount of the target nucleic acid in the biological sample.Type: ApplicationFiled: December 5, 2018Publication date: September 26, 2019Applicant: Esoterix Genetic Laboratories, LLCInventors: Thomas Scholl, Brant Hendrickson
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Patent number: 9777314Abstract: Methods for capturing and characterizing low frequency nucleic acid molecules indicative of diseases such as cancer (e.g. adenomas or early stage cancers) are provided. In some aspects, a low complexity capture technique is combined with a high complexity analytical technique. In some aspects, samples may be analyzed using a digital analysis and/or a single molecule sequencing technique.Type: GrantFiled: April 21, 2006Date of Patent: October 3, 2017Assignee: Esoterix Genetic Laboratories, LLCInventor: Anthony P. Shuber
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Patent number: 9361426Abstract: Systems and methods for analyzing copy number of a target locus, detecting a disease associated with abnormal copy number of a target gene or a carrier thereof.Type: GrantFiled: November 12, 2010Date of Patent: June 7, 2016Assignee: ESOTERIX GENETIC LABORATORIES, LLCInventors: Viatcheslav R. Akmaev, Brant Hendrickson, Thomas Scholl
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Patent number: 9109256Abstract: The invention provides methods for detecting and monitoring diseases associated with genetic abnormalities in a subject.Type: GrantFiled: October 27, 2005Date of Patent: August 18, 2015Assignee: Esoterix Genetic Laboratories, LLCInventor: Anthony P. Shuber
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Patent number: 8409829Abstract: Methods and compositions are provided for detecting the presence of nucleic acid sequence variants in a subpopulation of nucleic acid molecules in a biological sample. These methods are particularly useful for identifying individuals with mutations indicative of cancer.Type: GrantFiled: June 9, 2010Date of Patent: April 2, 2013Assignee: Esoterix Genetic Laboratories, LLCInventors: Anthony P. Shuber, Lisa Kann, Duncan Whitney
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Patent number: 8389220Abstract: Methods relating to isolating and amplifying chimeric nucleic acid molecules are provided. The methods of the invention are useful for detecting chromosome translocation events associated with diseases or conditions, such as cancer.Type: GrantFiled: July 18, 2011Date of Patent: March 5, 2013Assignee: Esoterix Genetic Laboratories, LLCInventor: Anthony P. Shuber
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Publication number: 20120264628Abstract: Embodiments of the present invention provide methods for the enrichment of rare microparticles, cells, or nucleic acids from a complex mixture using serial size exclusion filtration. Also provided are less invasive methods for detecting chromosomal or genetic abnormalities in a fetus, by enriching fetal microparticles in maternal plasma using serial size exclusion filtration, and isolating and analyzing the fetal nucleic acids from the fetal microparticles. Methods for diagnosis of diseases such as cancer are also provided, including enriching disease specific microparticles in the patient's plasma using serial size exclusion filtration, and isolating and analyzing the nucleic acids from the disease specific microparticles.Type: ApplicationFiled: January 31, 2012Publication date: October 18, 2012Applicant: Esoterix Genetic Laboratories, LLCInventors: Patricia Okamoto, Jan Godoski, Thomas Scholl
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Publication number: 20120196285Abstract: Methods for enriching specific microparticles, such as fetal microparticles or disease specific microparticles, in a biological sample are disclosed. In certain embodiments, the methods include combining a biological sample with a molecule that binds specific microparticles, and separating fractions of the biological sample, wherein the fraction that contains the binding molecule is enriched for the specific microparticles. Also disclosed are methods for enriching fetal nucleic acids by enriching fetal microparticles in a fraction of the biological sample and isolating nucleic acids from the enriched fraction. Methods for facilitating prenatal diagnosis of fetal chromosomal abnormalities are disclosed. In certain embodiments, the methods include combining a biological sample with a molecule that binds fetal microparticles, separating fractions of the biological sample, isolating nucleic acids from the fraction enriched for fetal microparticles, and analyzing the nucleic acids for the presence of a mutation.Type: ApplicationFiled: January 31, 2012Publication date: August 2, 2012Applicant: Esoterix Genetic Laboratories, LLCInventors: Patricia Okamoto, Jan Godoski, Thomas Scholl
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Patent number: 8067240Abstract: The present invention provides methods for aiding in the diagnoses of the neoplastic condition of a lung cell and methods of screening for a potential therapeutic agent for the reversal of the neoplastic condition.Type: GrantFiled: November 22, 2010Date of Patent: November 29, 2011Assignees: Esoterix Genetic Laboratories, LLC, Johns Hopkins University School of MedicineInventors: Jin Jen, Gary A. Beaudry, Stephen L. Madden, Arthur H. Bertlesen, David Sidransky
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Patent number: 7981607Abstract: Methods relating to isolating and amplifying chimeric nucleic acid molecules are provided. The methods of the invention are useful for detecting chromosome translocation events associated with diseases or conditions, such as cancer.Type: GrantFiled: August 29, 2005Date of Patent: July 19, 2011Assignee: Esoterix Genetic Laboratories LLCInventor: Anthony P. Shuber