Abstract: The present disclosure relates to devices, apparatus and methods of improving the accuracy of an image-based assay. One aspect of the present invention is to sandwich a sample between two plates and add reference marks in the sample areas of the plates, with at least one of the geometric and/optical properties of the reference marks being predetermined and known, and taking images of the sample with the reference marks, and applying a machine learning model in the analysis of the image-based assay.

Abstract: Disclosed are devices and methods for analyzing an analyte, such as white blood cells in liquid samples.

Abstract: The present invention is related to the field of bio/chemical sampling, sensing, assays and applications. Particularly, the present invention is related to how to make the sampling/sensing/assay become simple to use, fast to results, highly sensitive, easy to use, using tiny sample volume (e.g., 0.5 ?L or less), operated by a person without any professionals, reading by mobile-phone, or low cost, or a combination of them.

Abstract: The present invention is related to, among other things, the devices and methods that improve the accuracy and reliability of an assay, even when the assay device and/or the operation of the assay device has certain errors, and in some embodiments the errors are random.

Abstract: The present invention provides QMAX based devices, kits, and methods for rapid, easy to use, and/or inexpensive detection of assaying.

Abstract: Disclosed is an optical system for interrogating a sample, an optical system for measuring the spectrum of a beam of light, an optical system for measuring the spectrum of two beams of light, a compact imaging-based sensor or sensors, and combinations thereof.

Abstract: The present invention provides, among other things, the devices and methods that can rapidly change or cycle (i.e. heat and cool) a sample temperature with high speed, less heating energy, high energy efficiency, a compact and simplified apparatus (e.g. handheld), easy and fast operation, and/or low cost.

Abstract: The present invention provides devices, systems, and methods for rapid sample thermal cycle changes for the facilitation of reactions such as but not limited to PCR.

Abstract: The present disclosure relates to devices, apparatus and methods of improving the accuracy of image-based assay, that uses imaging system having uncertainties or deviations (imperfection) compared with an ideal imaging system. One aspect of the present invention is to add the monitoring marks on the sample holder, with at least one of their geometric and/optical properties of the monitoring marks under predetermined and known, and taking images of the sample with the monitoring marks, and train a machine learning model using the images with the monitoring mark.

Abstract: Among other things, the present invention is related to devices and methods of performing biological and chemical assays, such as but not limited to immunoassays and nucleic assay acid, particularly the homogeneous assay that does not use the step of wash and that is fast (e.g. 60 seconds from dropping a sample to displaying results).

Abstract: The disclosure relates to a device and method of using the device for performing biological and chemical assays that require an easy sample manipulation and/or a rapid change or a rapid thermal cycling of a sample temperature (e.g., Polymerase Chain Reaction (PCR) for amplifying nucleic acids). The device includes a first plate, a second plate, a plurality of spacers, and at least one clamp. The method includes obtaining the device, depositing a sample onto a sample contact area of at least one of the first and second plates of the device in an open configuration, closing the two plates into a closed configuration and placing the clamp in the active mode, and rapidly changing the temperature of the sample portion encircled by rings.

Abstract: Among other things, the present invention is related to devices and methods of performing biological and chemical assays, such as but not limited to immunoassays and nucleic assay acid, particularly the homogeneous assay that does not use the step of wash and that is fast (e.g. 60 seconds from dropping a sample to displaying results).

Abstract: The disclosure provides a kit and method using the kit for analyzing a sample, particularly blood. The kit may comprise a device (a) for sample collecting, mixing, staining, and transferring and a device (b) for sample distribution and imaging device. The device (a) comprises part (1) and an optional part (2) connected to each other. The part (1) is a capillary tube with reagent coated inside. The part (2) is used for providing an external force to suck in and/or push out the sample. The device (b) comprises two plates opposing to each other and defining an interior cavity, and a sample inlet. The interior cavity is in fluid communication with the exterior liquid sample contact area. The liquid sample flows into the spacing between the two plates through the sample inlet and a sample landing Zone that is at the exterior location of the device (b).

Abstract: Disclosed are devices, kits, apparatus, and methods for rapid homogenous cell staining and imaging.

Abstract: The disclosure provides an apparatus, a device, and methods for improving optical analysis of a thin layer of a sample between two plates, particularly for multiple wavelengths.

Abstract: The present invention provides, among other things, QMAX card based assays in different forms for various analytes, offering simpler, fast, more sensitive assaying.

Abstract: A device and system for changing the temperature of a fluidic sample includes a first plate and a second plate movable relative to each other into an open configuration and a closed configuration, and spacers having a uniform height. The plates include a sample contact area for contacting the sample and have a configuration for changing the temperature of the sample. In the open configuration the plates are separated, and the sample is capable of being deposited onto the plates. In the closed configuration, the plates are parallel, the plates compress the sample into a layer of uniform thickness that is stagnant relative to the plates, the layer is confined by the sample contact areas of the plates and is regulated by the plates and the spacers, and the plates are configured to change the temperature of the sample at a rate of at least 10° C./sec.

Abstract: Among other things, the present invention is related to bio/chemical sampling, sensing, assays and applications.

Abstract: The present invention is related to the field of bio/chemical sampling, sensing, assays and applications. Particularly, the present invention is related to how to make the sampling/sensing/assay become simple to use, fast to results, highly sensitive, easy to use, using tiny sample volume (e.g. 0.5 uL or less), operated by a person without any professionals, reading by mobile-phone, or low cost, or a combination of them.

Abstract: A homogeneous assay method that employs a device is provided. In some embodiments, the device contains a pair of plates that can be opened and closed. The sample is placed between two plates. In some embodiments, the thickness of the sample in a closed configuration, the concentration of labels, and amplification factor of the amplification surface are configured to make the label(s) bound on the amplification surface visible without washing away of the unbound labels.