Patents Assigned to Europaisches Laboratorium fur Molekularbiologie (EMBL)
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Publication number: 20120208277Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: ApplicationFiled: January 3, 2012Publication date: August 16, 2012Applicant: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Francis STEWART, Youming ZHANG, Frank BUCHHOLZ
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Publication number: 20120149060Abstract: The present invention relates to a nucleic acid containing at least one homing endonuclease site (HE) and at least one restriction enzyme site (X) wherein the HE and X sites are selected such that HE and X result in compatible cohesive ends when cut by the homing endonuclease and restriction enzyme, respectively, and the ligation product of HE and X cohesive ends can neither be cleaved by the homing endonuclease nor by the restriction enzyme. Further subject-matter of the present invention relates to a vector comprising the nucleic acid of the present invention, host cells containing the nucleic acid and/or the vector, a kit for cloning and/or expression of multiprotein complexes making use of the vector and the host cells, a method for producing a vector containing multiple expression cassettes, and a method for producing multiprotein complexes.Type: ApplicationFiled: March 8, 2010Publication date: June 14, 2012Applicant: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventor: Imre Berger
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Publication number: 20100317061Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: ApplicationFiled: April 9, 2010Publication date: December 16, 2010Applicant: EUROPAISCHES LABORATORIUM FUR MOLEKULARBIOLOGIE (EMBL)Inventors: FRANCIS STEWART, YOUMING ZHANG, FRANK BUCHHOLZ
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Patent number: 7736851Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: GrantFiled: May 11, 2004Date of Patent: June 15, 2010Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Francis Stewart, Youming Zhang, Frank Buchholz
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Patent number: 7312619Abstract: A local probe measuring device is provided for effecting local measurements of a sample. This device includes first and second local probes for local measurements with respect to a sample or a reference surface. A measurement condition adjustment arrangement is adapted to commonly adjust first and second measurement conditions of the respective first and second local probes with respect to the sample or the reference surface. A detection arrangement is provided that includes a first detection arrangement associated with the first local probe adapted to independently detect first measurement data referring to local measurements reflected by first local probe and a second detection arrangement associated with the second local probe adapted to independently detect second measurement data referring to local measurements effected by second local probe. Further, methods for effecting local measurements and local manipulations using multiple local probes are provided.Type: GrantFiled: July 17, 2006Date of Patent: December 25, 2007Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Stephan Maxmilian Altmann, Johann Karl Heinrich Hörber
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Publication number: 20060255818Abstract: The invention provides a local probe measuring device for effecting local measurements referring to a sample, comprising a first local probe for local measurements with respect to a sample or a reference surface, a second local probe for local measurements with respect to the sample or the reference surface, a measurement condition adjustment arrangement adapted to commonly adjust a first measurement condition of the first local probe with respect to the sample or the reference surface and a second measurement condition of the second local probe with respect to the sample or the reference surface, a detection arrangement comprising a first detection arrangement associated with the first local probe adapted to independently detect first measurement data referring to local measurements reflected by said first local probe and a second detection arrangement associated with the second local probe adapted to independently detect second measurement data referring to local measurements effected by said second local pType: ApplicationFiled: July 17, 2006Publication date: November 16, 2006Applicant: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Stephan Altmann, Johann Horber
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Patent number: 7098678Abstract: A local probe measuring device is provided for effecting local measurements of a sample. This device includes first and second local probes for local measurements with respect to a sample or a reference surface. A measurement condition adjustment arrangement is adapted to commonly adjust first and second measurement conditions of the respective first and second local probes with respect to the sample or the reference surface. A detection arrangement is provided that includes first detection arrangement associated with the first local probe adapted to independently detect first measurement data referring to local measurements reflected by first local probe and a second detection arrangement associated with the second local probe adapted to independently detect second measurement data referring to local measurements effected by second local probe. Further, methods for effecting local measurements and local manipulations using multiple local probes are provided.Type: GrantFiled: April 22, 2005Date of Patent: August 29, 2006Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Stephan Maxmilian Altmann, Johann Karl Heinrich Hörber
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Patent number: 6943574Abstract: The invention provides a local probe measuring device for effecting local measurements referring to a sample, having a first local probe for local measurements with respect to a sample, a second local probe for local measurements with respect to the sample, a measurement condition adjustment arrangement adapted to commonly adjust a first measurement condition of the first local probe with respect to the sample and a second measurement condition of the second local probe with respect to the sample, a detection arrangement having a first detection arrangement associated with the first local probe adapted to independently detect first measurement data referring to local measurements reflected by the first local probe and a second detection arrangement associated with the second local probe adapted to independently detect second measurement data referring to local measurements effected by the second local probe.Type: GrantFiled: September 17, 2004Date of Patent: September 13, 2005Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Stephan Maximilian Altmann, Johann Karl Heinrich Hörber
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Publication number: 20050184746Abstract: The invention provides a local probe measuring device for effecting local measurements referring to a sample, comprising a first local probe for local measurements with respect to a sample or a reference surface, a second local probe for local measurements with respect to the sample or the reference surface, a measurement condition adjustment arrangement adapted to commonly adjust a first measurement condition of the first local probe with respect to the sample or the reference surface and a second measurement condition of the second local probe with respect to the sample or the reference surface, a detection arrangement comprising a first detection arrangement associated with the first local probe adapted to independently detect first measurement data referring to local measurements reflected by said first local probe and a second detection arrangement associated with the second local probe adapted to independently detect second measurement data referring to local measurements effected by said second local pType: ApplicationFiled: April 22, 2005Publication date: August 25, 2005Applicant: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Stephan Altmann, Johann Karl Horbe
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Patent number: 6927399Abstract: A device is proposed for the precision rotation of samples on a diffractometer, especially for X-ray or synchrotron radiation diffraction experiments, comprising: a centering element (26) which is held at one end of a motor-driven rotating shaft (22) and can be displaced in a plane orthogonal to the axis of rotation of the rotating shaft (22), a sample holder (30) which is fixed to the centering element (26) or integral with the latter for holding a sample (32) substantially centrally with respect to the axis of rotation in an X-ray or synchrotron radiation beam (S), at least one micrometer finger (36) which is arranged in the region of the centering element (26) and can be positioned orthogonally with respect to the axis of rotation of the rotating shaft (22) by means of a micrometer finger drive device.Type: GrantFiled: July 22, 2003Date of Patent: August 9, 2005Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Florent Cipriani, Jean Charles Castagna
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Publication number: 20050040836Abstract: The invention provides a local probe measuring device for effecting local measurements referring to a sample, comprising a first local probe for local measurements with respect to a sample or a reference surface, a second local probe for local measurements with respect to the sample or the reference surface, a measurement condition adjustment arrangement adapted to commonly adjust a first measurement condition of the first local probe with respect to the sample or the reference surface and a second measurement condition of the second local probe with respect to the sample or the reference surface, a detection arrangement comprising a first detection arrangement associated with the first local probe adapted to independently detect first measurement data referring to local measurements reflected by said first local probe and a second detection arrangement associated with the second local probe adapted to independently detect second measurement data referring to local measurements effected by said second local pType: ApplicationFiled: September 17, 2004Publication date: February 24, 2005Applicant: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Stephan Altmann, Johann Horber
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Patent number: 6833923Abstract: A method for detecting an object and/or an object's ambience and/or an object's inner space using a scanning particle trapped within a trapping potential. The trapping potential is configured within a position range defined relative to the object or a reference point in a first position determining stage which is associated with a first order of magnitude. In a second position determining stage, which is associated with an order of magnitude less than that of the first order of magnitude, the scanning particle trapped within the trapping potential moves more or less freely in three-dimensions while scanning subjected to the trapping potential to scan the scan volume. A plurality of scanning particle positions assumed as a consequence of scanning are detected within the scan volume.Type: GrantFiled: November 15, 2001Date of Patent: December 21, 2004Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Ernst-Ludwig Florin, Heinrich J. K. Hörber, Ernst H. K. Stelzer
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Publication number: 20040203057Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: ApplicationFiled: May 11, 2004Publication date: October 14, 2004Applicant: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Francis Stewart, Youming Zhang, Frank Buchholz
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Patent number: 6798226Abstract: The invention provides a probe measuring device for effecting local measurements referring to a sample, having a first probe and a second probe, a measurement condition adjustment arrangement adapted to commonly adjust a first measurement condition of the first probe with respect to a sample or a reference surface and a second measurement condition of the second probe with respect to a sample or a reference surface, a detection arrangement having a first detection arrangement associated with the first probe adapted to independently detect first measurement data referring to local measurements effected by the first probe and a second detection arrangement associated with the second probe adapted to independently detect second measurement data referring to local measurements effected by the second probe. Also provided are methods for effecting local measurements and local manipulations using multiple probes.Type: GrantFiled: September 24, 2002Date of Patent: September 28, 2004Assignee: Europäisches Laboratorium für Molekularbiologie (EMBL)Inventors: Stephen Maximilian Altmann, Johann Karl Heinrich Hörber
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Patent number: 6787316Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: GrantFiled: August 30, 2002Date of Patent: September 7, 2004Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Francis Stewart, Youming Zhang, Frank Buchholz
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Patent number: 6627416Abstract: The invention relates to 5′-modified nucleotides and to nucleic acids which contain these nucleotides. Processes for incorporating the 5′-modified nucleotides into nucleic acids, and the subsequent site-specific cleavage of the nucleic acids at the 5′-modified monomer building blocks, are also disclosed. These processes can be employed for nucleic acid sequencing, for generating nucleic acid libraries, for detecting mutations, for preparing support-bound nucleic acids and for pharmaceutical purposes.Type: GrantFiled: January 10, 2001Date of Patent: September 30, 2003Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Konrad Faulstich, Wilhelm Ansorge
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Patent number: 6621085Abstract: A device is proposed for the precision rotation of samples on a diffractometer, especially for X-ray or synchrotron radiation diffraction experiments, comprising: a centering element (26) which is held at one end of a motor-driven rotating shaft (22) and can be displaced in a plane orthogonal to the axis of rotation of the rotating shaft (22), a sample holder (30) which is fixed to the centering element (26) or integral with the latter for holding a sample (32) substantially centrally with respect to the axis of rotation in an X-ray or synchrotron radiation beam (S), at least one micrometer finger (36) which is arranged in the region of the centering element (26) and can be positioned orthogonally with respect to the axis of rotation of the rotating shaft (22) by means of a micrometer finger drive device.Type: GrantFiled: June 28, 2001Date of Patent: September 16, 2003Assignee: Europäisches Laboratorium für Molekularbiologie (EMBL)Inventors: Florent Cipriani, Jean Charles Castagna
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Patent number: 6583411Abstract: The invention provides a local probe measuring device for effecting local measurements refering to a sample, comprising a plurality of local probes for local measurements with respect to a sample or a reference surface, a measurement condition adjustment arrangement adapted to commonly adjust measurement conditions of said local probes with respect to the sample or the reference surface, a plurality of detection arrangements, each being associated or adapted to be associated to one particular of said local probes and adapted to independently detect measurement data refering to local measurements effected by said particular local probe. Further, methods for effecting local measurements and local manipulations by means of multiple local probes are provided.Type: GrantFiled: September 13, 2000Date of Patent: June 24, 2003Assignee: Europaisches Laboratorium für Molekularbiologie (EMBL)Inventors: Stephan Maximilian Altmann, Johann Karl Heinrich Hörber
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Patent number: 6545492Abstract: A local probe measuring device is provided for effecting local measurements of a sample. This device includes first and second local probes for local measurements with respect to a sample or a reference surface. A measurement condition adjustment arrangement is adapted to commonly adjust first and second measurement conditions of the respective first and second local probes with respect to the sample or the reference surface. A detection arrangement is provided that includes first and second detection arrangements associated with the respective first and second local probes and adapted to independently detect first and second measurement data referring to local measurements effected by the respective first and second local probes.Type: GrantFiled: September 20, 1999Date of Patent: April 8, 2003Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Stephan Maximilian Altmann, Johann Karl Heinrich Hörber
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Publication number: 20030036198Abstract: The invention refers to a novel method for cloning DNA molecules using a homologous recombination mechanism between at least two DNA molecules comprising: a) providing a host cell capable of performing homologous recombination, b) contacting in said host cell a first DNA molecule which is capable of being replicated in said host cell with a second DNA molecule comprising at least two regions of sequence homology to regions on the first DNA molecule, under conditions which favour homologous recombination between said first and second DNA molecules and c) selecting a host cell in which homologous recombination between said first and second DNA molecules has occurred.Type: ApplicationFiled: August 30, 2002Publication date: February 20, 2003Applicant: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Francis Stewart, Youming Zhang, Frank Buchholz