Abstract: The invention provides a vaccine containing a proteinaceous material derived from the outer membrane of Bordetella pertussis, wherein the proteinaceous material is characterized by a relative molecular weight of 67,000 to about 73,000, preferably 69,000, as determined by 12% (w/w) polyacrylamide gel electrophoresis, and has a proline:glutamic acid ratio of about 1:1, in a pharmaceutically acceptable carrier or adjuvant. The invention also provides a method of inducing an immune response in an individual involving administering the vaccine of the invention to an individual.

Abstract: Expression of tetanus toxin fragment C is accomplished employing a DNA coding sequence having a (G+C)-content that has been increased in the region from nucleotide 410 to the 3' end of the coding sequence relative to the wild-type DNA sequence. This allows the production of complete mRNA transcripts. Typically the (G+C)-content is increased in the following regions: (i) nucleotides 510-710, (ii) nucleotides 650-850, (iii) nucleotides 800-1100, (iv) nucleotides 900-1200 and (v) nucleotides 1100-1356. These regions in wild-type DNA encompass terminator sequences.

Abstract: A process for the propagation of hepatitis A virus, which process comprises culturing cells infected with the virus in an aqueous culture medium in which the concentration of sodium chloride is from 30 mM to 170 mM above the isotonic concentration of sodium chloride; and production of a vaccine therefrom.

Abstract: Expression of tetanus toxin fragment C is accomplished employing a DNA coding sequence having a (G+C)-content that has been increased in the region from nucleotide 410 to the 3' end of the coding sequence relative to the wild-type DNA sequence. This allows the production of complete mRNA transcripts. Typically the (G+C)-content is increased in the following regions: (i) nucleotides 510-710, (ii) nucleotides 650-850, (iii) nucleotides 800-1100, (iv) nucleotides 900-1200 and (v) nucleotides 1100 to the 3' end of the coding sequence. The (G+C)-content may also be increased in the region of nucleotides 410-610. These regions in the wild-type DNA encompass terminator sequences.

Abstract: Tablets are coated by tumbling a batch of tablets in a rotating coating pan and subjecting the tablets while they are in the pan to a plurality of treatment steps. Each of these treatment steps consists of a plurality of treatment cycles each including a dosing period in which a measured quantity of tablet coating liquid is introduced into the pan, a drying period in which drying air is supplied to the interior of the pan and an intermediate mixing period during which the tablets tumble in contact with the already introduced coating liquid for a pre-set time and no drying air is supplied to the pan. The temperature of the batch of tablets being coated in the coating pan is sensed by a sensor. The temperature of the batch of tablets at the end of a pre-set time interval from the commencement of the drying period is stored in a memory device as a reference temperature.

Abstract: Tablets are coated by tumbling a batch of tablets in a rotating coating pan and subjecting the tablets while they are in the pan to a plurality of treatment steps. Each of these treatment steps consists of a plurality of treatment cycles each including a dosing period in which a measured quantity of tablet coating liquid is introduced into the pan, a drying period in which drying air is supplied to the interior of the pan and an intermediate mixing period during which the tablets tumble in contact with the already introduced coating liquid for a pre-set time and no drying air is supplied to the pan. The temperature of the batch of tablets being coating in the coating pan is sensed by a sensor. The temperature of the batch of tablets at the end of a pre-set time interval from the commencement of the drying period is stored in a memory device as a reference temperature.