Abstract: A microscope image of a cultured cell cluster derived from a cancer specimen of a patient is acquired. A measured value of a gene expression level of the cluster is acquired. Based on the image, a morphological representation identifiably expressing, by a vector quantity of a plurality of dimensions, a morphological difference between a group of cell clusters cultured from the same cancer specimen and a group of cell clusters cultured from another cancer specimen is acquired. The acquired morphological representation is input to a function, which is obtained by fitting the measured value with respect to the morphological representation, to acquire a prediction value of the gene expression level. Prediction accuracy is estimated based on the prediction value and the measured value. Based on the estimated prediction accuracy, a gene related to a morphological change of the cell cluster is extracted as a gene candidate.

Abstract: A data acquisition apparatus includes a light source, a first beam splitter, a predetermined beam splitter, a first light deflector, a second light deflector, a first measuring unit, a second measuring unit, a second beam splitter, and a photodetector. A second measurement optical path is positioned in a first direction and a reference optical path is positioned in a second direction. The predetermined beam splitter is disposed in the second measurement optical path or the reference optical path. A first measurement optical path is positioned between the predetermined beam splitter and the photodetector. The first light deflector and the first measuring unit are disposed in the first measurement optical path, and the second light deflector and the second measuring unit are disposed in the second measurement optical path. The first measurement optical path and the second measurement optical path intersect.

Abstract: A microscope apparatus includes a color temperature adjustment unit. The color temperature adjustment unit includes, in order from an incident side of light: a first polarizer that converts the light into linearly polarized light; a ¼-wave plate that converts at least light of a predetermined wavelength of the linearly polarized light into circularly polarized light, the ¼-wave plate being fixed in a predetermined orientation relative to the first polarizer; and a second polarizer that extracts a predetermined polarization component, the second polarizer being disposed to be rotatable relative to the first polarizer. A difference between a phase amount with respect to light of 435 nm and a phase amount with respect to light of 635 nm of the ¼-wave plate is 30 degrees or more.

Abstract: A sample observation apparatus includes a light source unit, an illumination optical system, an observation optical system, a light detection element, a scanning unit, a holding member, and an image processing device. A light spot is formed by the illumination optical system. The scanning unit moves the light spot and the holding member relative to each other. A pupil of the observation optical system and the light detection element are positioned at a position conjugate to a pupil position of the illumination optical system. The image processing device generates an image of a sample based on a predetermined image and a filter, and the predetermined image is an image based on a signal output from the light detection element. The filter includes a first region and a second region, and a value in the first region is greater than a value in the second region.

Abstract: An objective includes a positive first lens group, a negative second lens group having one or more aspheric surface, a third lens group, and a positive fourth lens group consists of two lens components; wherein, 1.6?fL/TTL?5??(1) 1.5?ER1/ER2??(2) D2/OTTL?0.6??(3) are satisfied. Herein, fL is a focal length. TTL is the distance from the surface closest to the object side to the surface closest to the image side. ER1 is an effective radius of the surface closest to the object side. ER2 is an effective radius of the surface of the second lens group closest to the object side. D2 is the distance from the object plane to the surface of the second lens group closest to the image side. OTTL is the distance from the object plane to the surface closest to the image side.

Abstract: An experiment support apparatus includes: one or more non-transitory computer-readable media that include an instruction; and one or more processors that execute the instruction. The instruction is configured to cause the one or more processors to execute an operation, the operation includes: causing a display device to display a condition table TB that indicates an experiment condition for measurement, in response to an input of the experiment condition; and causing the display device to display at least one of a measurement result based on measurement data, or an analysis result of the measurement data, in a cell of the condition table TB, in response to an input of the measurement data, the measurement data being obtained using a measurement apparatus under the experiment condition corresponding to the cell.

Abstract: A processor causes a storage medium to store three-dimensional data of a subject in a storage step. The processor selects a reference image in a first selection step. The processor selects a selected image that is a two-dimensional image used for generating the three-dimensional data on the basis of the reference image in a second selection step. The processor estimates a second camera coordinate regarding the reference image on the basis of a first camera coordinate regarding the selected image in an estimation step. The processor displays an image of the subject on a display in a display step. The image of the subject visualizes at least one of the second camera coordinate and a set of three-dimensional coordinates of one or more points of the subject calculated on the basis of the second camera coordinate.

Abstract: In a three-dimensional image display method, a processor acquires first three-dimensional data and second three-dimensional data of a subject from a recording medium. The processor converts a first three-dimensional coordinate system of the first three-dimensional data and a second three-dimensional coordinate system of the second three-dimensional data into a three-dimensional common coordinate system on the basis of structure information related to a geometric structure of the subject. The processor displays an image of the first three-dimensional data in the common coordinate system and an image of the second three-dimensional data in the common coordinate system on a display.

Abstract: A three-dimensional reconstruction device includes a processor. The processor is configured to acquire position-and-orientation information and two-dimensional coordinate information. The processor is configured to acquire a three-dimensional size of a subject. The processor is configured to calculate a correction coefficient used for matching the size at the position of a camera to a known size. The processor is configured to correct the position of the camera by using the correction coefficient. The processor is configured to restore a three-dimensional shape of the subject by using the corrected position. the orientation of the camera at the position, and the two-dimensional coordinate information.

Abstract: A cell analyzing device includes a control circuit. The control circuit acquires data of an observation image. The control circuit specifies a colony of a cultured cell in the observation image. The control circuit calculates a ratio at which the cultured cell occupies a predetermined range included in the observation image as an occupancy rate. The control circuit calculates a size of the colony. The control circuit determines a state of the cultured cell based on the occupancy rate and the size of the colony.

Abstract: The image processing system includes a scanner, a pixelated photon detector (PPD), and at least one processor. The at least one processor displays a setting screen on a display unit. The setting screen is a screen for setting an identification range that is a range of gradation to be identified. The at least one processor displays a second image obtained by converting a first image on the display unit. The second image is an image obtained by converting the first image based on at least the identification range. The first image is generated based on an intensity signal of light detected by a PPD and a scanning position by the scanner.

Abstract: An image processing method includes: generating two images by performing two image enhancement processes for a microscopic image; and generating a corrected image obtained by compositing the two images, wherein the generating the two images includes generating a high-frequency enhanced image in which high-frequency components of the microscopic image are enhanced relative to low-frequency components of the microscopic image that have a lower frequency than the high-frequency components, and generating a microstructure enhanced image in which a microstructure in an observation sample included in the microscopic image is enhanced.

Abstract: A microscopic examination device includes: a camera that images an observation area of an examination subject observed with a microscope device so as to acquire an image of the observation area; and one or more processors including hardware, the one or more processors being configured to: align the image of the observation area with an area in a reference image, the area corresponding to the image of the observation area; generate a navigation map from the reference image by recording, on the reference image, a position of the image of the observation area in the reference image; calculate a direction of movement to an unobserved area in the navigation map, the unobserved area being an area where the position of the image of the observation area is not recorded; and present an access method to the unobserved area on a basis of the direction of movement.

Abstract: An optical scanning apparatus includes: a light source that emits light; a basic wavefront modulator that performs spatial light phase modulation on incident light on an optical path from the light source to an incident end surface of a fiber, to thereby generate light having a basic wavefront and emit the generated light, the light having the basic wavefront being to be incident on the incident end surface for obtaining, by light emitted from an emission end surface of the fiber, illumination light having a basic intensity distribution; and a two-dimensional intensity distribution control apparatus configured to change the basic intensity distribution by emitting light for causing light in which a phase distribution of the basic wavefront is controlled to be incident on the incident end surface of the fiber.

Abstract: An illumination optical system for endoscope includes a rod lens as an optical element. The optical element includes a proximal end surface through which light enters and a distal end surface configured to emit the light. The distal end surface includes a diffusion region configured to diffuse emitted light. The diffusion region includes a plurality of concave portions and a plurality of peripheral regions surrounding the concave portions. Each concave portion includes a plurality of inclined surfaces as total reflection surfaces that are inclined with respect to the distal end surface. Each peripheral region includes a transmission surface configured to emit light totally reflected by the total reflection surfaces after passing through the proximal end surface and light not totally reflected by the total reflection surfaces.

Abstract: An observation system includes a mounting table on which a sample container is placed, a surface light source that is disposed in one of two regions divided by the mounting table and has a light emitting plane, an observation optical system disposed in the other thereof, a conveyance mechanism moving the observation optical system in a direction orthogonal to an optical axis of the observation optical system to change an observation position, and a controller controlling a light emission pattern defined by a light emitting region where light is emitted on the light emitting plane. The controller executes first light emission pattern control in which the light emission pattern is changed according to the observation position, or, alternatively, second light emission pattern control in which the light emission pattern is switched between a plurality of periodic light emission patterns having phases different from each other.

Abstract: A measurement system includes: an optical measurement instrument that measures a surface of a specimen; and a control apparatus that controls the measurement instrument. The control apparatus includes: one or more non-transitory computer-readable media that include an instruction; and one or more processors that execute the instruction. The instruction is configured to cause the one or more processors to execute an operation. The operation includes: causing the measurement instrument to repetitively measure the surface of the specimen without changing the setting; and evaluating appropriateness of the setting for measuring surface texture of the specimen, based on comparison between a measurement value of the surface texture of the specimen calculated from a measurement data item output from the measurement instrument, and a degree of variation in measurement by the measurement instrument calculated from a plurality of measurement data items output from the measurement instrument.

Abstract: A microscope system comprising an eyepiece, an objective that guides light from a sample to the eyepiece, a tube lens that is disposed on a light path between the eyepiece and the objective and forms an optical image of the sample on the basis of light therefrom, a projection apparatus that projects a projection image including a first assistance image onto an image plane on which the optical image is formed, and a processor that performs processes. The processes include generating projection image data representing the projection image. The first assistance image is an image of the sample in which a region wider than an actual field of view corresponding to the optical image is seen, The first assistance image is projected onto a portion of the image plane that is close to an outer edge of the optical image.

Abstract: An insertion assisting instrument has an outer diameter dimension set to satisfy a relationship of xb?<xc? assuming that an insertion assisting instrument is projected on X-Y coordinates such that a point on a circumference where the insertion assisting instrument has a largest outer diameter (that is, a point on an outer periphery of a proximal-end-side tapered surface at a distal end) is set as an origin and the proximal-end-side tapered surface is brought into contact with an X axis, coordinates of a second point B, which is most distal from the X axis on the circumference where the insertion assisting instrument has the largest outer diameter are (xb?, yb?), and coordinates of a third point C, which is most proximal from the X axis on a circumference at the proximal end of the insertion assisting instrument, are (xc?, yc?).

Abstract: A refractive index distribution estimating system includes an illumination optical system configured to illuminate a sample, an imaging optical system configured to form an optical sample image, an image sensor configured to capture optical images of the sample, and a processor configured to reconstruct a refractive index distribution of the sample from images.