Patents Assigned to Exact Science Corporation
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Patent number: 9710596Abstract: Provided herein is technology relating to evaluating the state of nucleic acids and particularly, but not exclusively, to methods for measuring variations between DNAs, including differences in methylation and mutation.Type: GrantFiled: November 21, 2012Date of Patent: July 18, 2017Assignee: EXACT SCIENCES CORPORATIONInventor: Graham Lidgard
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Patent number: 9657330Abstract: Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting multiple DNA targets from human stool samples.Type: GrantFiled: July 10, 2014Date of Patent: May 23, 2017Assignee: Exact Sciences CorporationInventors: Graham P. Lidgard, Janelle J. Bruinsma, Michael J. Domanico, Hemanth Shenoi
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Patent number: 9637792Abstract: The present invention relates to methods and compositions for determination of and uses of specific methylation patterns indicative of adenoma and carcinoma. In particular, the invention relates to analysis of defined CpG loci that are coordinately methylated in DNAs from cancer and adenoma samples, methods for identifying coordinately methylated loci, and methods of using analysis of coordinately methylated loci in one or more marker regions in the design of assays for adenoma and cancer.Type: GrantFiled: February 2, 2012Date of Patent: May 2, 2017Assignees: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH, EXACT SCIENCES CORPORATIONInventors: David A. Ahlquist, William R. Taylor, Hongzhi Zou, Graham P. Lidgard
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Patent number: 9631228Abstract: Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.Type: GrantFiled: May 15, 2015Date of Patent: April 25, 2017Assignee: EXACT SCIENCES CORPORATIONInventors: Janelle J. Bruinsma, Michael J. Domanico, Graham P. Lidgard, Hongzhi Zou, William G. Weisburg, Hemanth D. Shenoi, James P. Light, II, Keith Kopitzke, John Zeis
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Publication number: 20160273030Abstract: The present invention provides DNA plasmids that find use as calibrators or reference standards for calculating DNA quantities in a sample. In particular, provided herein are DNA plasmids that contain multiple control fragments, and methods of their use in DNA quantitation.Type: ApplicationFiled: November 4, 2014Publication date: September 22, 2016Applicant: Exact Sciences CorporationInventors: Michael Domanico, Ilse A. Tyler, Brian Aizenstein, Hatim Allawi, Graham P. Lidgard
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Publication number: 20160206293Abstract: Provided herein is technology relating to collecting and containing samples and particularly, but not exclusively, to technology for collecting and containing a stool specimen. The technology provides a device for collecting and containing a stool specimen, the device comprising ergonomic features optimized for a geriatric user. The technology also provides an enclosing holder for securing the ergonomic stool specimen container in a leak-proof manner e.g., during mechanical shaking.Type: ApplicationFiled: August 29, 2014Publication date: July 21, 2016Applicant: Exact Sciences CorporationInventors: Joseph E. Schiestle, Krystal A. Bruger, Timothy A. Parmer, Jad A. Lunde, Sam M. Jang, David A. Schuelke, David B. Wolgemuth, Jing Yang Lu
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Patent number: 9376721Abstract: A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3? terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3? terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.Type: GrantFiled: March 27, 2014Date of Patent: June 28, 2016Assignee: EXACT SCIENCES CORPORATIONInventors: Hongzhi Zou, Graham P. Lidgard, Michael J. Domanico, Hatim Allawi
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Patent number: 9315853Abstract: Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfate conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.Type: GrantFiled: January 30, 2013Date of Patent: April 19, 2016Assignee: EXACT SCIENCES CORPORATIONInventors: Michael J. Domanico, Hatim Allawi, Graham P. Lidgard, Brian Aizenstein, Oliver Hunt, Tobias Charles Zutz
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Patent number: 9290797Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.Type: GrantFiled: December 19, 2012Date of Patent: March 22, 2016Assignee: Exact Sciences CorporationInventors: Rebecca Oldham-Haltom, Hongzhi Zou, Graham P. Lidgard, Michael J. Domanico, Hatim Allawi
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Patent number: 9211112Abstract: Provided herein is technology relating to collecting and preparing samples. For example, the technology relates particularly, but not exclusively, to devices, systems, and kits that allow a subject to collect and prepare a sample for analysis.Type: GrantFiled: August 16, 2012Date of Patent: December 15, 2015Assignee: EXACT SCIENCES CORPORATIONInventors: Graham P. Lidgard, Michael J. Domanico, Keith Fourrier, James P. Light, II, Scott Castanon, Keith Kopitzke
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Patent number: 9169511Abstract: Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.Type: GrantFiled: December 31, 2013Date of Patent: October 27, 2015Assignee: EXACT SCIENCES CORPORATIONInventors: Janelle J. Bruinsma, Michael J. Domanico, Graham P. Lidgard, Hongzhi Zou, William G. Weisburg, Hemanth D. Shenoi, James P. Light, II, Keith Kopitzke, John Zeis
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Patent number: 9163278Abstract: Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.Type: GrantFiled: December 31, 2013Date of Patent: October 20, 2015Assignee: EXACT SCIENCES CORPORATIONInventors: Janelle J. Bruinsma, Michael J. Domanico, Graham P. Lidgard, Hongzhi Zou, William G. Weisburg, Hemanth D. Shenoi, James P. Light, II, Keith Kopitzke, John Zeis
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Patent number: 9127318Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.Type: GrantFiled: August 24, 2012Date of Patent: September 8, 2015Assignee: EXACT SCIENCES CORPORATIONInventors: Rebecca Oldham-Haltom, Hatim Allawi, Hongzhi Zou, Michael J. Domanico, Graham P. Lidgard
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Patent number: 9121071Abstract: A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3? terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3? terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.Type: GrantFiled: March 27, 2014Date of Patent: September 1, 2015Assignee: Exact Sciences CorporationInventors: Hongzhi Zou, Graham P. Lidgard, Michael J. Domanico, Hatim Allawi
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Patent number: 9057098Abstract: Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.Type: GrantFiled: December 31, 2013Date of Patent: June 16, 2015Assignee: Exact Sciences CorporationInventors: Janelle J. Bruinsma, Hemanth D. Shenoi, Michael J. Domanico, James P. Light, II, Keith Kopitzke, John Zeis
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Patent number: 9024006Abstract: A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3? terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3? terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.Type: GrantFiled: March 14, 2014Date of Patent: May 5, 2015Assignee: Exact Sciences CorporationInventors: Hongzhi Zou, Graham P. Lidgard, Michael J. Domanico, Hatim Allawi
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Patent number: 9000146Abstract: Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting multiple DNA targets from a human stool sample with, for example, methods employing spin filtration techniques.Type: GrantFiled: May 11, 2012Date of Patent: April 7, 2015Assignee: Exact Sciences CorporationInventors: Janelle J. Bruinsma, Michael J. Domanico, Graham P. Lidgard, Hongzhi Zou, William G. Weisburg, Hemanth D. Shenoi, James P. Light, II
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Patent number: 8999176Abstract: Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.Type: GrantFiled: December 31, 2013Date of Patent: April 7, 2015Assignee: Exact Sciences CorporationInventors: Michael J. Domanico, James P. Light, II, Keith Kopitzke, John Zeis
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Patent number: 8993341Abstract: Provided herein is technology relating to processing and preparing samples and particularly, but not exclusively, to methods, systems, and kits for removing assay inhibitors, e.g, compounds that inhibit polymerase chain reaction, from samples comprising nucleic acids. In particular, the technology is directed toward treating crude sample preparations, such as supernatants from homogenized stool samples, with insoluble polyvinylpyrrolidone (PVP) to form PVP-assay inhibitor complexes, and spin filtration to separate the PVP-assay inhibitor complexes from the crude sample preparations to produce clarified samples that exhibit reduced assay inhibition.Type: GrantFiled: May 11, 2012Date of Patent: March 31, 2015Assignee: Exact Sciences CorporationInventors: Janelle J. Bruinsma, Hemanth D. Shenoi, Michael J. Domanico, James P. Light, II
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Patent number: 8980107Abstract: Provided herein is technology relating to filtration and particularly, but not exclusively, to filters and methods for filtering by means of centrifugation.Type: GrantFiled: May 11, 2012Date of Patent: March 17, 2015Assignee: Exact Sciences CorporationInventors: Micheal J. Domanico, James P. Light, II, Keith Koptizke, John Zeis