Abstract: The present invention provides an insect infection method for use in the production of a protein with a baculovirus expression vector in the insect, the method comprising the steps of: (g) providing a plurality of insect larvae or pupae; (h) providing a solution comprising a wild type baculovirus or a baculovirus expression vector having a desired gene encoding a protein; (i) stressing the insect larvae or pupae; (j) soaking the insect larvae or pupae in the solution for an appropriate time so that they are infected with the wild type baculovirus or the baculovirus expression vector; and (k) incubating the infected larvae or pupae for production of the protein; and (l) harvesting the protein. The method can treat a great quantity of larvae simultaneously, achieve batch infection of larvae or pupae, save manpower and effectively infect larvae or pupae at a high infection rate.
Abstract: The present invention discloses an airflow capture booth with single-plate windbreak comprising an extracting means for extracting polluted air having an opening for air contamination extracting through and a crosswind device for simulating crosswind, the improvement comprises that said crosswind device having a single-plate windbreak perpendicular to an airflow direction of said crosswind having a specific distance to said opening of said extracting means for forming a capture zone, wherein the airflow of said capture zone is not easy to be shed and is extracted mostly by said extracting means.
Type:
Application
Filed:
June 17, 2002
Publication date:
December 18, 2003
Applicants:
Institute of Occupation Safety and Health, Council of Labor Affairs, Executive Yuan
Inventors:
Rong Fung Huang, Yu-Kang Chen, Wen-Yu Yeh, Chun-Wan Chen, Jin Hsun Liu