Abstract: Provided is a method of improving skin condition by applying to the skin of a subject a cosmetic composition comprising black yeast-derived exosomes as an active ingredient and thereby improving the skin condition, particularly skin elasticity improvement, skin wrinkle reduction, skin texture improvement, skin tone improvement, skin brightness improvement, skin regeneration, skin moisturization, and/or whitening.

Abstract: A method is provided for producing exosomes containing an overexpressed CD64 or a portion thereof. The method includes transducing a genetic construct encoding a full-length CD64 or a portion thereof into cells that do not naturally express CD64 or a portion thereof, without fusion with a nucleic acid sequence encoding an exogenous transmembrane protein or a transmembrane domain thereof; expressing the genetic construct in the cells; culturing the cells in a medium; and isolating exosomes secreted or released into the cell culture medium.

Abstract: A composition containing a combination of exosomes and sapogenin as an active ingredient is disclosed. The composition is able to promote preadipocyte proliferation, lipid uptake into adipocytes, and/or adipogenesis, but reduce the cytotoxicity caused by sapogenin. Therefore, the composition can reduce side effects on the body or skin, and can be used such that it is conveniently applied to a dissatisfactory part of the body showing flaws which look less plump due to a deficit in lipids and the like, or an area of the skin showing flaws caused by a deficit in lipids.

Abstract: A composition for alleviating facial redness containing stem cell-derived exosomes as an active ingredient and a method of reducing facial redness are provided. When a facial skin is treated with the composition for alleviating facial redness containing stem cell-derived exosomes as an active ingredient, the composition exhibits an excellent effect on skin care by reducing the redness of the face and improving the appearance of the face.

Abstract: A cosmetic composition containing rose stem cell-derived exosomes as an active ingredient is provided for whitening a skin. The cosmetic composition has excellent effects on skin whitening, skin beauty and the like.

Abstract: A cosmetic composition containing rose stem cell-derived exosomes as an active ingredient is provided for skin regeneration, skin elasticity improvement or skin wrinkle reduction. The cosmetic composition has excellent effects on skin regeneration, skin elasticity improvement and/or skin wrinkle reduction.

Abstract: A composition containing edelweiss-derived exosomes as an active ingredient is provided for skin elasticity improvement, skin wrinkle reduction, skin regeneration, anti-inflammation, wound healing or wound healing acceleration. The composition is less likely to contain impurities such as growth regulation factors than conditioned media of edelweiss callus, or filtrates or extracts thereof, and has excellent effects on skin elasticity improvement, skin wrinkle reduction, skin regeneration, anti-inflammation, wound healing and/or wound healing acceleration.

Abstract: The present invention provides a composition for preventing, ameliorating, alleviating or treating dermatitis comprising exosomes derived from adipose-derived stem cells as an active ingredient. The composition of the present invention is able to act against dermatitis-inducing multiple cytokine targets, and thus be widely applied against dermatitis caused by various factors and effectively suppress and alleviate dermatitis.

Abstract: A composition containing rose stem cell-derived exosomes as an active ingredient is provided for anti-inflammation, wound healing or wound healing acceleration. The composition has excellent effects on anti-inflammation, wound healing and/or wound healing acceleration.

Abstract: A cosmetic composition including exosomes derived from Galactomyces as an active ingredient is provided for skin regeneration, skin elasticity improvement or skin wrinkle reduction. The cosmetic composition has excellent effects on skin regeneration, skin elasticity improvement and/or skin wrinkle reduction.

Abstract: A composition for strengthening skin barrier or improving skin barrier function is able to improve objective indicators related to the protection of skin barrier, the strengthening of skin barrier, and/or the improvement of skin barrier function. The composition exhibits the effects of increasing the amount of ceramides, dihydroceramides and sphingoid bases, increasing the activities of enzymes that are involved in the synthesis thereof, and decreasing the activities of enzymes that are involved in the degradation thereof. In addition, the composition is able to restore skin barrier function by reducing TSLP, IL-4, and IL-13 which are closely associated with skin barrier damage, and thus interrupting a vicious circle in which the lipids and proteins contributing to skin barrier decrease.

Abstract: The lyophilized formulation of stem cell-derived exosomes and the anti-inflammatory composition including the same as an active ingredient is able to stabilize stem cell-derived exosomes and exhibit excellent anti-inflammatory effects, and particularly, exhibit remarkable anti-inflammatory effects as compared with not-lyophilized stem cell-derived exosomes isolated and purified from conditioned media of stem cells. Therefore, the lyophilized formulation of stem cell-derived exosomes and the anti-inflammatory composition including the same as an active ingredient is able to effectively prevent, suppress, alleviate, ameliorate or treat inflammatory response or inflammatory diseases.

Abstract: The present invention provides a composition for preventing, suppressing, alleviating, ameliorating or treating pruritus comprising stem cell-derived exosomes as an active ingredient. The composition of the present invention is able to act against pruritus-inducing multiple cytokine targets, for example, IL-4, IL-31 and TSLP, and thus is able to be widely applied against pruritus caused by various factors and is able to effectively suppress and alleviate pruritus. In addition, when the composition of the present invention is applied directly to human skin, it is able to remarkably ameliorate pruritus-associated clinical scores, erythema and the like. Thus, the composition of the present invention is able to be used as a pharmaceutical composition, a skin external preparation and a cosmetic composition for preventing, suppressing, alleviating, ameliorating or treating pruritus.

Abstract: A method of lyophilizing exosomes using a cryoprotectant comprising methionine, mannitol and trehalose is disclosed. The lyophilized exosome product shows a good appearance which maintains a porous sponge shape without forming ice crystals. In addition, the lyophilized exosome product can be applied to a pharmaceutical composition, a skin external preparation and a cosmetic composition. For example, the lyophilized exosome product can be used as a solution obtained by simply mixing it with a diluent.

Abstract: A method for producing exosomes is provided. The method includes culturing animal cells in a medium containing TNF-? and interferon-?. This method is possible not only to increase production of exosomes to be isolated from a cell-derived conditioned medium, but also to exhibit an excellent effect of enhancing bioactivity of exosomes, such as increasing elastin synthesis of exosomes.

Abstract: A method for promoting production of exosomes and/or extracellular vesicles is provided. The method includes culturing animal cells in a medium containing ascorbic acid, an analogue thereof, or a derivative thereof. The method is able to enhance the productivity of exosomes and/or extracellular vesicles secreted or released per cell or cell-derived conditioned medium.

Abstract: A cosmetic composition including exosomes derived from Galactomyces as an active ingredient is provided for skin regeneration, skin elasticity improvement or skin wrinkle reduction. The cosmetic composition has excellent effects on skin regeneration, skin elasticity improvement and/or skin wrinkle reduction.

Abstract: A cosmetic composition containing rose stem cell-derived exosomes as an active ingredient is provided for skin regeneration, skin elasticity improvement or skin wrinkle reduction. The cosmetic composition has excellent effects on skin regeneration, skin elasticity improvement and/or skin wrinkle reduction.

Abstract: A composition for alleviating facial redness containing stem cell-derived exosomes as an active ingredient and a method of reducing facial redness are provided. When a facial skin is treated with the composition for alleviating facial redness containing stem cell-derived exosomes as an active ingredient, the composition exhibits an excellent effect on skin care by reducing the redness of the face and improving the appearance of the face.

Abstract: A method of lyophilizing exosomes using a cryoprotectant comprising methionine, mannitol and trehalose is disclosed. The lyophilized exosome product shows a good appearance which maintains a porous sponge shape without forming ice crystals. In addition, the lyophilized exosome product can be applied to a pharmaceutical composition, a skin external preparation and a cosmetic composition. For example, the lyophilized exosome product can be used as a solution obtained by simply mixing it with a diluent.