Abstract: The current invention provides a method for determining the metastatic potential, capability, status, or characteristics of a primary tumor from a human cancer patient by determining expression patterns of proteins that initiate, cause, promote, mediate, inflict, or otherwise aid metastatic properties of cells from a primary tumor. The identification of one or more proteins associated with metastasis in a single primary tumor can determine whether a primary tumor is metastatic or has the potential to become metastatic.
Abstract: The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solublization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.