Abstract: The invention relates to a method comprising the following steps: a) use of a support which has at least two different microparticle populations immobilized thereon; b) measuring the fluorescence of the support from step a) with an optical resolution 1, said resolution 1 permitting differentiation of microparticle singlets, doublets, triplets, multiplets and monolayers and determination of the localized position of individual immobilized microparticles; c) contacting the support from step a) with the sample to be analyzed; d) performing at least one additional measurement of the fluorescence of the support during or after contacting in accordance with step c) with a resolution 2; e) assigning the fluorescence values measured with resolution 2 to the individual microparticle singlets, doublets, triplets, multiplets and monolayers locally identified on the support in accordance with step b) and assigned to a particular acceptor molecule population; f) determining the change in fluorescence.

Abstract: The present invention relates to a suspension culture process for producing an enzyme, comprising the steps of providing a minimal medium, which contains deionised water, mineral salts and an organic carbon source, in a vessel; inoculating the minimal medium with a fungus; incubating the minimal medium at a pH value of 1-4 for a period of time which is sufficient for the fungus to be optically visible in the minimal medium, and obtaining the enzyme, wherein the minimal medium and the vessel are not sterilised. In addition, the invention relates to an enzyme obtainable by the process according to the invention.