Abstract: The present invention refers to an acceleration process for macromolecular fragmentation in liquid medium, in gel or in a solid support, by means of joint application of enzymes and ultrasounds, as well as isotopes. The macromolecules, in any of these liquids, are mixed with enzymes and inserted in a recipient (2) and exposed to an ultrasonic radiation field (1). The combined effect of the enzymes and of the ultrasounds allows accomplishing, in time ranges between 10 s and 600 s, the fragmentation of macromolecules or whole proteontes, with the correspondent formation of their constituent lower molecular units.
Type:
Application
Filed:
July 7, 2006
Publication date:
October 1, 2009
Applicants:
FACULDADE DE CIENCIA E TECHNOLOGIA-UNIVERSIDADE NOVA DE LISBOA, CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Inventors:
Jose Luis Capelo Martinez, Jesus Vazquez Cobos, Daniel Lopez Ferrer, Isabel Moura