Abstract: The present invention relates to non-invasive methods, kits and means for diagnosing and/or prognosing of glioblastoma in a body fluid sample from a subject. Further, the present invention relates to set of polynucleotides or sets of primer pairs for detecting sets of miRNAs for diagnosing and/or prognosing of glioblastoma in a body fluid sample from a subject. In addition, the present invention relates to sets of miRNAs for diagnosing and/or prognosing of glioblastoma in a body fluid sample from a subject.

Abstract: The present invention relates to non-invasive methods, kits and means for diagnosing and/or prognosing of prostate diseases in a body fluid sample from a subject. Further, the present invention relates to set of polynucleotides or sets of primer pairs for detecting sets of miRNAs for diagnosing and/or prognosing of prostate diseases in a body fluid sample from a subject. In addition, the present invention relates to sets of miRNAs for diagnosing and/or prognosing of prostate diseases in a body fluid sample from a subject.

Abstract: The present invention relates to single polynucleotides or sets of polynucleotides for detecting single miRNAs or sets of miRNAs for diagnosing and/or prognosing of a lung disease in a biological sample from a patient. Further, the present invention relates to means for diagnosing and/or prognosing of a lung disease comprising said polynucleotides or sets of polynucleotides. Furthermore, the present invention relates to a method for diagnosing and/or prognosing of a lung disease based on the determination of expression profiles of single miRNAs or sets of miRNAs representative for a lung disease. In addition, the present invention relates to a kit for diagnosing and/or prognosing of a lung disease comprising means for determining expression profiles of single miRNAs or sets of miRNAs representative for a lung disease and optionally at least one reference and/or algorithm or mathematical function comprised on at least one data carrier.

Abstract: MicroRNAs (miRNA) are a recently discovered class of small non-coding RNAs (17-14 nucleotides). Due to their function as regulators of gene expression they play a critical role both in physiological and in pathological processes, such as cancer. The present invention provides novel methods for diagnosing a state of health based on the determination of specific miRNAs that have altered expression levels in different conditions, e.g. disease states compared to healthy controls.

Abstract: The invention relates to methods and devices for preparing synthetic nucleic acids.

Abstract: The invention relates to an extraction method for isolating target molecules from a sample using a microfluidic carrier.

Abstract: The invention relates to a method for producing polymers, in particular synthetic nucleic acid double strands of optional sequence, comprising the steps: (a) providing a support having a surface area which contains a plurality of individual reaction areas, (b) location-resolved synthesizing nucleic acid fragments each having different base sequences in several of the individual reaction areas, and (c) detaching the nucleic acid fragments from individual reaction areas.

Abstract: The present invention relates to arrays of biochemical and/or biofunctional elements such as nucleic acids (oligonucleotides, for example) or other biomolecules on a carrier surface and methods of producing such arrays using photoactivation of predetermined areas for synthesis using an illumination matrix that is computer-controlled to generate an exposure pattern. This exposure pattern can be adjusted and monitored by computer using a light sensor matrix, for example a CCD matrix, to allow precise, controlled illumination of specific regions and therefore attachment of array building blocks to those specific regions. The methods and compositions of the invention permit spatially resolved photochemical synthesis of polymer probes on a carrier.

Abstract: The present invention relates to a process for synthesizing biopolymers by stepwise assembly from protected synthesis building blocks which carry two-stage protective groups. The two-stage protective groups are activated by a first illumination step and eliminated by a subsequent chemical treatment step. Photoactivatable components which considerably speed up the activation process via intramolecular triplet sensitizers or/and have fluorescence properties are used. The two-stage protective groups can be used in particular within the framework of quality control.

Abstract: The invention relates to a method for producing polymers, in particular synthetic nucleic acid double strands of optional sequence, comprising the steps: (a) provision of a support having a surface area which contains a plurality of individual reaction areas, (b) location-resolved synthesis of nucleic acid fragments having in each case different base sequences in several of the individual reaction areas, and (c) detachment of the nucleic acid fragments from individual reaction areas.