Abstract: A kit for determining for a female subject the implantation potential of embryos obtained or to be obtained by assisted fertilization is described. The kit includes at least one reagent suitable for detection of levels of FF G-CSF or FF G-CSF mRNA, such as anti-G-CSF antibody or a nucleic acid probe for detection of levels of G-CSF mRNA. The kit may also include a set of concentration standards of FF G-CSF and aspirator tips for removing an oocyte and follicular fluid.

Abstract: The present invention relates to an assay for determining the implantation potential of a plurality of embryos each obtained or to be obtained by assisted fertilization of an oocyte of a female subject, comprising measuring the levels of granulocyte-colony stimulating factor (G-CSF) in the follicular fluid (FF) present in the follicle from which each oocyte is derived, and determining the implantation potential of each embryo from the level of measured FF G-CSF. It also relates to a kit for carrying out the assay. It further relates to a method for assisted fertilization.